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Research Article

In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins

Yu Lv1,§, Mingshi Deng1,§, Xuefeng Wang2, Xinghua Gao1, Lingyan Feng1, Wei Chen3( ), Chung-Chiun Liu4, Xinxin Li2, Yuan Zhang1( )
Materials Genome Institute, Shanghai University, Shanghai 200444, China
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
Department of Emergency, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China
Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
Yu Lv and Mingshi Deng contributed equally to this paper.
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Graphical Abstract

We herein report a rational engineering strategy for the construction of an integrated electrochemical microsensing platform capable of direct profiling serum markers of inflammation. This method provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections.

Abstract

To address the challenge of highly sensitive and accurate detection of biomarkers in complex environments, a rational engineering strategy for designing electrochemical immunosensing platform is proposed. Herein, we develop a microsensor chip through the combination of multiplexed electrodes and microfluidic channels for the parallel detection of human interleukins (IL-6 and IL-8). For the construction of an efficient sensing interface, the conductive silver nanowires (Ag NWs) wrapped with zeolitic imidazolate framework (ZIF-8) thin film (denoted as ZIF-8@Ag NWs) are prepared, and then employed for the multi-functionalization of electrodes. The immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ ions in ZIF-8 films with biomolecules, which offer the high assay sensitivity and good specificity. The immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex media. Further clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic accuracy. Principal component analysis (PCA) reveals that 18 patient samples could be fully separated from healthy control samples. The low-cost, easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections.

Keywords

zeolitic imidazolate framework (ZIF)integrated nanostructuremicrosensorbiomarker detectioninterleukins

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Nano Research
Volume 16 Issue 2,
February 2023
Pages 2866-2874
DOI: 10.1007/s12274-022-5030-y
Cite this article:
Lv Y, Deng M, Wang X, et al. In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins. Nano Research, 2023, 16(2): 2866-2874. https://doi.org/10.1007/s12274-022-5030-y
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Received: 10 July 2022
Revised: 06 September 2022
Accepted: 09 September 2022
Published: 07 October 2022
© Tsinghua University Press 2022
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