AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Research Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article

In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins

Yu Lv1,§, Mingshi Deng1,§, Xuefeng Wang2, Xinghua Gao1, Lingyan Feng1, Wei Chen3( ), Chung-Chiun Liu4, Xinxin Li2, Yuan Zhang1( )
Materials Genome Institute, Shanghai University, Shanghai 200444, China
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
Department of Emergency, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China
Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
Yu Lv and Mingshi Deng contributed equally to this paper.
Show Author Information

Graphical Abstract

We herein report a rational engineering strategy for the construction of an integrated electrochemical microsensing platform capable of direct profiling serum markers of inflammation. This method provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections.

Abstract

To address the challenge of highly sensitive and accurate detection of biomarkers in complex environments, a rational engineering strategy for designing electrochemical immunosensing platform is proposed. Herein, we develop a microsensor chip through the combination of multiplexed electrodes and microfluidic channels for the parallel detection of human interleukins (IL-6 and IL-8). For the construction of an efficient sensing interface, the conductive silver nanowires (Ag NWs) wrapped with zeolitic imidazolate framework (ZIF-8) thin film (denoted as ZIF-8@Ag NWs) are prepared, and then employed for the multi-functionalization of electrodes. The immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ ions in ZIF-8 films with biomolecules, which offer the high assay sensitivity and good specificity. The immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex media. Further clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic accuracy. Principal component analysis (PCA) reveals that 18 patient samples could be fully separated from healthy control samples. The low-cost, easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections.

Keywords

zeolitic imidazolate framework (ZIF)integrated nanostructuremicrosensorbiomarker detectioninterleukins

Electronic Supplementary Material

Download File(s)
12274_2022_5030_MOESM1_ESM.pdf (1.1 MB)

References

[1]

Turner, M. D.; Nedjai, B.; Hurst, T.; Pennington, D. J. Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochim. Biophys. Acta 2014, 1843, 2563–2582.
Crossref Google Scholar
[2]

Lippitz, B. E. Cytokine patterns in patients with cancer: A systematic review. Lancet Oncol. 2013, 14, e218–e228.
Crossref Google Scholar
[3]

Sheu, B. C.; Chang, W. C.; Cheng, C. Y.; Lin, H. H.; Chang, D. Y.; Huang, S. C. Cytokine regulation networks in the cancer microenvironment. Front. Biosci. 2008, 13, 6255–6268.
Crossref Google Scholar
[4]

Akdis, M.; Aab, A.; Altunbulakli, C.; Azkur, K.; Costa, R. A.; Crameri, R.; Duan, S.; Eiwegger, T.; Eljaszewicz, A.; Ferstl, R. et al. Interleukins (from IL-1 to IL-38), interferons, transforming growth factor β, and TNF-α: Receptors, functions, and roles in diseases. J. Allergy Clin. Immunol. 2016, 138, 984–1010.
Crossref Google Scholar
[5]

Klein, T.; Wang, W.; Yu, L. N.; Wu, K.; Boylan, K. L. M.; Vogel, R. I.; Skubitz, A. P. N.; Wang, J. P. Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers. Biosens. Bioelectron. 2019, 126, 301–307.
Crossref Google Scholar
[6]

Sun, D.; Cao, F. H.; Xu, W. Q.; Chen, Q. D.; Shi, W.; Xu, S. P. Ultrasensitive and simultaneous detection of two cytokines secreted by single cell in microfluidic droplets via magnetic-field amplified SERS. Anal. Chem. 2019, 91, 2551–2558.
Crossref Google Scholar
[7]

Russell, C.; Ward, A. C.; Vezza, V.; Hoskisson, P.; Alcorn, D.; Steenson, D. P.; Corrigan, D. K. Development of a needle shaped microelectrode for electrochemical detection of the sepsis biomarker interleukin-6 (IL-6) in real time. Biosens. Bioelectron. 2019, 126, 806–814.
Crossref Google Scholar
[8]

Malhotra, R.; Patel, V.; Chikkaveeraiah, B. V.; Munge, B. S.; Cheong, S. C.; Zain, R. B.; Abraham, M. T.; Dey, D. K.; Gutkind, J. S.; Rusling, J. F. Ultrasensitive detection of cancer biomarkers in the clinic by use of a nanostructured microfluidic array. Anal. Chem. 2012, 84, 6249–6255.
Crossref Google Scholar
[9]

Chen, R. P.; Du, X.; Cui, Y. J.; Zhang, X. Y.; Ge, Q. Y.; Dong, J.; Zhao, X. W. Vertical flow assay for inflammatory biomarkers based on nanofluidic channel array and SERS nanotags. Small 2020, 16, 2002801.
Crossref Google Scholar
[10]

Wang, X.; Wang, Q. X.; Wang, Q. H.; Gao, F.; Gao, F.; Yang, Y. Z.; Guo, H. X. Highly dispersible and stable copper terephthalate metal-organic framework-graphene oxide nanocomposite for an electrochemical sensing application. ACS Appl. Mater. Interfaces 2014, 6, 11573–11580.
Crossref Google Scholar
[11]

Ying, Z.; Feng, L. Y.; Ji, D. Q.; Zhang, Y.; Chen, W.; Dai, Y. F.; Janyasupab, M.; Li, X. X.; Wen, W. J.; Liu, C. C. Phase-regulated sensing mechanism of MoS2 based nanohybrids toward point-of-care prostate cancer diagnosis. Small 2020, 16, 2000307.
Crossref Google Scholar
[12]

Wehmeyer, K. R.; White, R. J.; Kissinger, P. T.; Heineman, A. R. In electrochemical affinity assays/sensors: Brief history and current status. Annu. Rev. Anal. Chem. 2021, 14, 109–131.
Crossref Google Scholar
[13]

Ge, X. X.; Xia, Z. H.; Guo, S. J. Recent advances on black phosphorus for biomedicine and biosensing. Adv. Funct. Mater. 2019, 29, 1900318.
Crossref Google Scholar
[14]

Xu, K.; Zhang, B. Y.; Mohiuddin, M.; Ha, N. M.; Wen, X. M.; Zhou, C. H.; Li, Y. X.; Ren, G. H.; Zhang, H. J.; Zavabeti, A. et al. Free-standing ultra-thin Janus indium oxysulfide for ultrasensitive visible-light-driven optoelectronic chemical sensing. Nano Today 2021, 37, 101096.
Crossref Google Scholar
[15]

Wongkaew, N.; Simsek, M.; Griesche, C.; Baeumner, A. J. Functional nanomaterials and nanostructures enhancing electrochemical biosensors and lab-on-a-chip performances: Recent progress, applications, and future perspective. Chem. Rev. 2019, 119, 120–194.
Crossref Google Scholar
[16]

Ji, D. Q.; Ying, Z.; Zhang, Y.; Chen, W.; Janyasupab, M.; Gao, X. H.; Feng, L. Y.; Wen, W. J. RhIr@MoS2 nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum. Chin. Chem. Lett. 2020, 31, 2115–2118.
Crossref Google Scholar
[17]

Tite, T.; Chiticaru, E. A.; Burns, J. S.; Ioniţă, M. Impact of Nano-morphology, lattice defects and conductivity on the performance of graphene based electrochemical biosensors. J. Nanobiotechnol. 2019, 17, 101.
Crossref Google Scholar
[18]

Hasanzadeh, M.; Shadjou, N.; de la Guardia, M. Nanosized hydrophobic gels: Advanced supramolecules for use in electrochemical bio- and immunosensing. TrAC Trends Anal. Chem. 2018, 102, 210–224.
Crossref Google Scholar
[19]

Zhang, Y.; Wei, Q. The role of nanomaterials in electroanalytical biosensors: A mini review. J. Electroanal. Chem. 2016, 781, 401–409.
Crossref Google Scholar
[20]

Su, S.; Sun, Q.; Gu, X. D.; Xu, Y. Q.; Shen, J. L.; Zhu, D.; Chao, J.; Fan, C. H.; Wang, L. H. Two-dimensional nanomaterials for biosensing applications. TrAC Trends Anal. Chem. 2019, 119, 115610.
Crossref Google Scholar
[21]

Vikrant, K.; Tsang, D. C. W.; Raza, N.; Giri, B. S.; Kukkar, D.; Kim, K. H. Potential utility of metal-organic framework-based platform for sensing pesticides. ACS Appl. Mater. Interfaces 2018, 10, 8797–8817.
Crossref Google Scholar
[22]

Xu, X. Y.; Ji, D. Q.; Zhang, Y.; Gao, X. H.; Xu, P. C.; Li, X. X.; Liu, C. C.; Wen, W. J. Detection of phenylketonuria markers using a ZIF-67 encapsulated PtPd alloy nanoparticle (PtPd@ZIF-67)-based disposable electrochemical microsensor. ACS Appl. Mater. Interfaces 2019, 11, 20734–20742.
Crossref Google Scholar
[23]

Chen, W.; Ji, D. Q.; Zhang, Y.; Xu, P. C.; Gao, X. H.; Fang, J. H.; Li, X. X.; Feng, L. Y.; Wen, W. J. Schiff-base reaction induced selective sensing of trace dopamine based on a Pt41Rh59 alloy/ZIF-90 nanocomposite. Nanotechnology 2019, 30, 335708.
Crossref Google Scholar
[24]

Rezki, M.; Septiani, N. L. W.; Iqbal, M.; Harimurti, S.; Sambegoro, P.; Adhika, D. R.; Yuliarto, B. Amine-functionalized Cu-MOF nanospheres towards label-free hepatitis B surface antigen electrochemical immunosensors. J. Mater. Chem. B 2021, 9, 5711–5721.
Crossref Google Scholar
[25]

Duan, F. H.; Hu, M. Y.; Guo, C. P.; Song, Y. P.; Wang, M. H.; He, L. H.; Zhang, Z. H.; Pettinari, R.; Zhou, L. M. Chromium-based metal–organic framework embedded with cobalt phthalocyanine for the sensitively impedimetric cytosensing of colorectal cancer (CT26) cells and cell imaging. Chem. Eng. J. 2020, 398, 125452.
Crossref Google Scholar
[26]

Meng, L. Y.; Turner, A. P. F.; Mak, W. C. Conducting polymer-reinforced laser-irradiated graphene as a heterostructured 3D transducer for flexible skin patch biosensors. ACS Appl. Mater. Interfaces 2021, 13, 54456–54465.
Crossref Google Scholar
[27]

Zhao, Y.; Wang, X. J.; Yang, S. Z.; Kuttner, E.; Taylor, A. A.; Salemmilani, R.; Liu, X.; Moskovits, M.; Wu, B. H.; Dehestani, A. et al. Protecting the nanoscale properties of Ag nanowires with a solution-grown SnO2 monolayer as corrosion inhibitor. J. Am. Chem. Soc. 2019, 141, 13977–13986.
Crossref Google Scholar
[28]

Iijima, M.; Kuroda, S. I. Scaffolds for oriented and close-packed immobilization of immunoglobulins. Biosens. Bioelectron. 2017, 89, 810–821.
Crossref Google Scholar
[29]

Rahman, M.; Cui, D. X.; Zhou, S. K.; Zhang, A. M.; Chen, D. A graphene oxide coated gold nanostar based sensing platform for ultrasensitive electrochemical detection of circulating tumor DNA. Anal. Methods 2020, 12, 440–447.
Crossref Google Scholar
[30]

Han, R.; Wang, G. X.; Xu, Z. Y.; Zhang, L. Y.; Li, Q.; Han, Y. F.; Luo, X. L. Designed antifouling peptides planted in conducting polymers through controlled partial doping for electrochemical detection of biomarkers in human serum. Biosens. Bioelectron. 2020, 164, 112317.
Crossref Google Scholar
[31]

Shamsuddin, S. H.; Gibson, T. D.; Tomlinson, D. C.; McPherson, M. J.; Jayne, D. G.; Millner, P. A. Reagentless affimer- and antibody-based impedimetric biosensors for CEA-detection using a novel non-conducting polymer. Biosens. Bioelectron. 2021, 178, 113013.
Crossref Google Scholar
[32]

Wu, Q.; Li, N. B.; Wang, Y.; Liu, Y.; Xu, Y. C.; Wei, S. T.; Wu, J. D.; Jia, G. R.; Fang, X. D.; Chen, F. F. et al. A 2D transition metal carbide MXene-based SPR biosensor for ultrasensitive carcinoembryonic antigen detection. Biosens. Bioelectron. 2019, 144, 111697.
Crossref Google Scholar
[33]

Wang, Z. H.; Yang, S. N.; Wang, Y. Y.; Feng, W.; Li, B. S.; Jiao, J.; Han, B. K.; Chen, Q. A novel oriented immunosensor based on AuNPs-Thionine-CMWCNTs and staphylococcal protein A for interleukin-6 analysis in complicated biological samples. Anal. Chim. Acta 2020, 1140, 145–152.
Crossref Google Scholar
[34]

Agarwal, G.; Naik, R. R.; Stone, M. O. Immobilization of histidine-tagged proteins on nickel by electrochemical dip pen nanolithography. J. Am. Chem. Soc. 2003, 125, 7408–7412.
Crossref Google Scholar
[35]

Isaksen, M. L.; Fitzgerald, K. Purification and analysis of antibody fragments using proteins L, A and LA. In Antibody Engineering. Kontermann, R.; Dübel, S., Eds.; Springer: Berlin, 2001, 282–291.
Crossref Google Scholar
[36]

Christopoulos, T. K.; Diamandis, E. P. Immunoassay configurations. In Immunoassay. Diamandis, E. P.; Christopoulos, T. K., Eds.; San Diego: Academic Press, 1996, 227–236.
Crossref Google Scholar
[37]

Baraket, A.; Lee, M.; Zine, N.; Sigaud, M.; Bausells, J.; Errachid, A. A fully integrated electrochemical biosensor platform fabrication process for cytokines detection. Biosens. Bioelectron. 2017, 93, 170–175.
Crossref Google Scholar
[38]

Tertis, M.; Leva, P. I.; Bogdan, D.; Suciu, M.; Graur, F.; Cristea, C. Impedimetric aptasensor for the label-free and selective detection of interleukin-6 for colorectal cancer screening. Biosens. Bioelectron. 2019, 137, 123–132.
Crossref Google Scholar
[39]

Tanak, A. S., Muthukumar, S., Krishnan, S., Schully, K. L., Clark, D. V., Prasad, S. Multiplexed cytokine detection using electrochemical point-of-care sensing device towards rapid sepsis endotyping. Biosens Bioelectron 2021, 171, 112726.
Crossref Google Scholar
[40]

Tatara, R.; Karayaylali, P.; Yu, Y.; Zhang, Y. R.; Giordano, L.; Maglia, F.; Jung, R.; Schmidt, J. P.; Lund, I.; Shao-Horn, Y. The effect of electrode–electrolyte interface on the electrochemical impedance spectra for positive electrode in Li-ion battery. J. Electrochem. Soc. 2018, 166, A5090–A5098.
Crossref Google Scholar
[41]

Chandra Barman, S.; Sharifuzzaman, M.; Zahed, M. A.; Park, C.; Yoon, S. H.; Zhang, S. P.; Kim, H.; Yoon, H.; Park, J. Y. A Highly selective and stable cationic polyelectrolyte encapsulated black phosphorene based impedimetric immunosensor for interleukin-6 biomarker detection. Biosens. Bioelectron. 2021, 186, 113287.
Crossref Google Scholar
[42]

Zhang, C. Y.; Shi, D. M.; Li, X. Y.; Yuan, J. Microfluidic electrochemical magnetoimmunosensor for ultrasensitive detection of interleukin-6 based on hybrid of AuNPs and graphene. Talanta 2022, 240, 123173.
Crossref Google Scholar
[43]

Molano Franco, D.; Arevalo Rodriguez, I.; Roqué I Figuls, M.; Zamora, J. Interleukin-6 for diagnosis of sepsis in critically ill adult patients. Cochrane. Db. Syst. Rev. 2015, 7, CD011811.
Crossref Google Scholar
[44]

Miguel-Bayarri, V.; Casanoves-Laparra, E. B.; Pallás-Beneyto, L.; Sancho-Chinesta, S.; Martín-Osorio, L. F.; Tormo-Calandín, C.; Bautista-Rentero, D. Prognostic value of the biomarkers procalcitonin, interleukin-6 and C-reactive protein in severe sepsis. Med. Intensiva (Engl. Ed.) 2012, 36, 556–562.
Crossref Google Scholar
[45]

Wong, D. T. Towards a simple, saliva-based test for the detection of oral cancer: ‘Oral fluid (saliva), which is the mirror of the body, is a perfect medium to be explored for health and disease surveillance’. Expert Rev. Mol. Diagn. 2006, 6, 267–272.
Crossref Google Scholar
[46]

Nißler, R.; Bader, O.; Dohmen, M.; Walter, S. G.; Noll, C.; Selvaggio, G.; Groß, U.; Kruss, S. Remote near infrared identification of pathogens with multiplexed nanosensors. Nat. Commun. 2020, 11, 5995.
Crossref Google Scholar
Nano Research
Volume 16 Issue 2,
February 2023
Pages 2866-2874
DOI: 10.1007/s12274-022-5030-y
Cite this article:
Lv Y, Deng M, Wang X, et al. In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins. Nano Research, 2023, 16(2): 2866-2874. https://doi.org/10.1007/s12274-022-5030-y
Topics:
Medical applications

1367

Views

2

Crossref

2

Web of Science

2

Scopus

0

CSCD

Altmetrics
Received: 10 July 2022
Revised: 06 September 2022
Accepted: 09 September 2022
Published: 07 October 2022
© Tsinghua University Press 2022
Return