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Original Research | Open Access

Identification of 3-ketocapnine reductase activity within the human microbiota

Xiaotong Wu1,2,#,, Lukuan Hou2,3,#, Haili Zhang2, Yi Ma1, Jufang Wang1, Mingwei Cai2( ), Xiaoyu Tang2 ( )
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, China
College of Chemistry and Pharmacy, Northwest A&F University, Yangling, China

#Xiaotong Wu and Lukuan Hou contributed equally to this study.

Editor: Chenli Liu, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, China

Show Author Information

Abstract

The microbial synthesis of sulfonolipids within the human body is likely involved in maintaining human health or causing diseases. However, the enzymes responsible for their biosynthesis remain largely unknown. In this study, we identified and verified the role of 3-ketocapnine reductase, the third-step enzyme, in the four-step conversion of L-phosphoserine into sulfobacin B both in vivo and in vitro. This finding builds upon our previous research into sulfonolipid biosynthesis, which focused on the vaginal bacterium Chryseobacterium gleum DSM 16776 and the gut bacterium Alistipes finegoldii DSM 17242. Through comprehensive gene mapping, we demonstrate the widespread presence of potential sulfonolipid biosynthetic genes across diverse bacterial species inhabiting various regions of the human body. These findings shed light on the prevalence of sulfonolipid-like metabolites within the human microbiota, suggesting a potential role for these lipid molecules in influencing the intricate biointeractions within the complex microbial ecosystem of the human body.

Keywords

3-ketocapnine reductasecapnineshuman microbiotasulfonolipids

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mLife
Volume 3 Issue 2,
June 2024
Pages 307-316
DOI: 10.1002/mlf2.12134
Cite this article:
Wu X, Hou L, Zhang H, et al. Identification of 3-ketocapnine reductase activity within the human microbiota. mLife, 2024, 3(2): 307-316. https://doi.org/10.1002/mlf2.12134

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Received: 28 December 2023
Accepted: 25 April 2024
Published: 28 June 2024
© 2024 The Author(s). mLife published by John Wiley & Sons Australia, Ltd on behalf of Institute of Microbiology, Chinese Academy of Sciences.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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