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Fluorophore–Photochrome Co-Embedded Polymer Nanoparticles for Photoswitchable Fluorescence Bioimaging
Shouzhi Pu2(
), Fuyou Li1()
), Fuyou Li1()Abstract
Photoswitchable polymer nanoparticles (ps-PNPs) have been constructed by emulsion polymerization through embedding an iridium(Ⅲ) complex, [Ir(bt)2(pic)] (bt = phenylbenzothiazole, pic = picolinate), as a fluorophore and a diarylethene derivative (DTE) as a photochrome. The ps-PNPs show reversible luminescence modulation with DTE switching using UV light in aqueous solution and even in living cells. Such luminescent modulation only occurred in polymer nanoparticles and not in a simple mixed solution with low concentrations of [Ir(bt)2(pic)] and DTE. The ps-PNPs displayed good cellular uptake and fast intracellular luminescence modulation by photoswitching, indicating the nanoparticles could be developed as photoswitchable luminescent probes for a diversity of bioimaging applications.
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Pages 494-503
Cite this article:
Yang T, Liu Q, Pu S, et al. Fluorophore–Photochrome Co-Embedded Polymer Nanoparticles for Photoswitchable Fluorescence Bioimaging. Nano Research, 2012, 5(7): 494-503. https://doi.org/10.1007/s12274-012-0235-0
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