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Research Article

AS1411 aptamer-conjugated Gd2O3: Eu nanoparticles for target-specific computed tomography/magnetic resonance/fluorescence molecular imaging

Tsungrong Kuo1,2, Weiyun Lai3,4, Chenghung Li1, Yanjhan Wun1, Huancheng Chang2, Jinnshiun Chen5, Panchyr Yang6, Chiachun Chen1,2( )
Department of Chemistry"National Taiwan Normal University", Taipei 116TaiwanChina
Institute of Atomic and Molecular Science"Academia Sinica", Taipei, 106TaiwanChina
Molecular Medicine ProgramTaiwan International Graduate ProgramInstitute of Biomedical Sciences"Academia Sinica", Taipei, 115TaiwanChina
Institute of Biochemistry and Molecular BiologySchool of Life Sciences"National Yang-Ming University", Taipei, 112TaiwanChina
Division of Colorectal SurgeryChang Gung Memorial HospitalChang Gung University, Taoyuan, 333TaiwanChina
College of Medicine"National Taiwan University", Taipei, 106TaiwanChina
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Graphical Abstract

Abstract

Europium-doped gadolinium oxide (Gd2O3: Eu) nanoparticles have been synthesized, and then their surfaces have been conjugated with nucleolintargeted AS1411 aptamer to form functionalized target-specific Gd2O3: Eu nanoparticles (A-GdO: Eu nanoparticles). The A-GdO: Eu nanoparticles present strong fluorescence in the visible range, high magnetic susceptibility, X-ray attenuation and good biocompatibility. The A-GdO: Eu nanoparticles have been applied to test molecular expression of nucleolin highly expressed CL1-5 lung cancer cells under a confocal microscope. Fluorescence imaging clearly reveals that the nanoparticles can be applied as fluorescent tags for cancer-targeting molecular imaging. Furthermore, taking together their excellent T1 contrast and strong computed tomography (CT) signal, the A-GdO: Eu nanoparticles demonstrate a great capability for use as a dual modality contrast agent for CT and magnetic resonance (MR) molecular imaging. Animal experiments also show that the A-GdO: Eu nanoparticles are able to contrast the tissues of BALB/c mice using CT modality. Moreover, the obvious red fluorescence of A-GdO: Eu nanoparticles can be visualized in a tumor by the naked eye. Overall, our results demonstrate that the A-GdO: Eu nanoparticles can not only serve as new medical contrast agents but also as intraoperative fluorescence imaging probes for guided surgery in the near future.

Keywords

aptamercontrast agentGd2O3 nanoparticlesmolecular imagingnanoparticles synthesis

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Nano Research
Volume 7 Issue 5,
May 2014
Pages 658-669
DOI: 10.1007/s12274-014-0420-4
Cite this article:
Kuo T, Lai W, Li C, et al. AS1411 aptamer-conjugated Gd2O3: Eu nanoparticles for target-specific computed tomography/magnetic resonance/fluorescence molecular imaging. Nano Research, 2014, 7(5): 658-669. https://doi.org/10.1007/s12274-014-0420-4

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Received: 08 July 2013
Revised: 10 January 2014
Accepted: 16 January 2014
Published: 23 April 2014
© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014
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