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Research Article

Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery

Li Li1, Wenyi Gu1, Jian Liu2, Shiyu Yan1, Zhi Ping Xu1( )
Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandBrisbaneQLD4072Australia
Department of Chemical EngineeringCurtin UniversityPerthWA6845Australia
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Graphical Abstract

Abstract

In this work a novel strategy has been developed to prepare well-dispersed amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposite (NH2-SiO2@LDH) via electrostatic interactions and condensation of (3-aminopropyl)triethoxysilane (APTES). This nanocomposite system is well dispersed in culture media and phosphate buffered saline, and exhibits low cytotoxicity and good biocompatibility. The fluorescence microscopy images and flow cytometry data indicate that such an NH2-SiO2@LDH nanocomposite is able to efficiently deliver small interfering RNA (siRNA) into the U2OS cell line to inhibit cell proliferation. Thus, NH2-SiO2@LDH nanocomposite has a great potential as a nanocarrier for efficient gene delivery.

Keywords

layered double hydroxide (LDH)functionalizationself-assemblygene delivery

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Nano Research
Volume 8 Issue 2,
February 2015
Pages 682-694
DOI: 10.1007/s12274-014-0552-6
Cite this article:
Li L, Gu W, Liu J, et al. Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery. Nano Research, 2015, 8(2): 682-694. https://doi.org/10.1007/s12274-014-0552-6

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Received: 27 May 2014
Revised: 20 July 2014
Accepted: 31 July 2014
Published: 10 September 2014
© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014
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