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Research Article

Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap

Murtaza Hasan1Wenlong Yang1Yanmin Ju1Xin Chu1Yun Wang2Yulin Deng2Nasir Mahmood1Yanglong Hou1( )
Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China
School of Life Sciences Beijing Institute of Technology Beijing 100081 China
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Abstract

Recently, magnetic nanoparticles (NPs) have been extensively used in food industry and biomedical treatments. However, the biocompatibility mechanism on expression proteomics, before consideration of magnetic NPs for clinical application, has not yet been fully elucidated. Therefore, this study was undertaken to identify potential biomarkers of metal ion signaling proteins in human cervical cancer cell line (HeLa) cells. Here, we report the in vitro investigations of the cell cycle response and significant changes in protein abundance of HeLa cells when exposed to self-tailored hydrophilic Fe2C NPs. The comparative proteomic approach based on 18O labeling coupled with high performance liquid chromatography/electrospray ionization with ion trap mass analyzer (HPLC/ESI-Orbitrap) was applied, and 394 proteins were identified. There were 46 significantly differentiated proteins based on the specific metal ion signaling response. Among them, 60S ribosomal protein L37a, serine/arginine-rich splicing factor 7, calmodulin, and calumenin were downregulated, whereas transketolase was overexpressed. Functional interaction network of Fe2C-regulated proteins was successfully created by the STRING algorithm to show the strong interactions between proteins. This work will not only help to understand the molecular mechanism of metal ion signaling proteins that can potentially be used to develop therapeutic protocols for diagnosis of diseases but also give direction for tailoring biocompatible magnetic NPs.

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Nano Research
Pages 1912-1923
Cite this article:
Hasan M, Yang W, Ju Y, et al. Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap. Nano Research, 2017, 10(6): 1912-1923. https://doi.org/10.1007/s12274-016-1375-4

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Received: 27 June 2016
Revised: 12 November 2016
Accepted: 15 November 2016
Published: 05 January 2017
© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016
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