AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Research Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article

Enhanced RuBisCO activity and promoted dicotyledons growth with degradable carbon dots

Hao Li1, Jian Huang2, Yang Liu1( ), Fang Lu4, Jun Zhong1, Yong Wang3, Shuiming Li3, Yeshayahu Lifshitz1,5( ), Shuit-Tong Lee1, Zhenhui Kang1( )
Institute of Functional Nano & Soft Materials (FUNSOM),Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Joint International Research Laboratory of Carbon-Based Functional Materials and Devices, Soochow University,Suzhou,215123,China;
School of biology & basic medical sciences,Soochow university,Suzhou,215123,China;
College of Life Science and Oceanography,Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University,Shenzhen,518060,China;
School of Basic Medical Sciences,Beijing University of Chinese Medicine,Beijing,100029,China;
Department of Materials Science and Engineering, Technion,Israel Institute of Technology,Haifa,3200003,Israel;
Show Author Information

Graphical Abstract

Abstract

The ~ 5 nm degradable carbon dots (CDs) were synthesized directly from carbon rod by a one-step electrochemical method at room temperature. The as-prepared CDs can effectively enhance the ribulose bisphosphate carboxylase oxygenase (RuBisCO) activity, and then promote the dicotyledons growth (soybean, tomato, eggplant and so on) and finally increase their yields. Here, we used Arabidopsis thaliana and Trifolium repens L. as model plants to systematically study the beneficial effects of CDs on plant growth. These include: (i) accelerating seed germination; (ii) enlarging root elongation; (iii) increasing metal ions absorption and delivery; (iv) improving enzymes activity; (v) enhancing the carbohydrate content; (vi) degradation into plant hormone analogues and CO2; and finally (vii) enhancing the grain production by about 20%.

Keywords

carbon dotsbio-degradationphotosynthesisplant life cycleimprove growth

Electronic Supplementary Material

Download File(s)
12274_2019_2397_MOESM1_ESM.pdf (6.3 MB)

References

1

Hamilton, A.; Hamilton, P. Plant Conservation: An Ecosystem Approach; Earthscan: London, 2006.
Google Scholar
2

Kesler, S. E.; Simon, A. C. Mineral Resources, Economics and the Environment; Cambridge University: Cambridge, 2015.
Crossref
3

Zheng, L.; Hong, F. S.; Lu, S. P.; Liu, C. Effect of nano-TiO2 on strength of naturally aged seeds and growth of spinach. Biol. Trace Elem. Res. 2005, 104, 83-91.
Crossref Google Scholar
4

Hong, F. S.; Zhou, J.; Liu, C.; Yang, F.; Wu, C.; Zheng, L.; Yang, P. Effect of nano-TiO2 on photochemical reaction of chloroplasts of spinach. Biol. Trace Elem. Res. 2005, 105, 269-279.
Crossref Google Scholar
5

Gao, F. Q.; Hong, F. S.; Liu, C.; Zheng, L.; Su, M. Y.; Wu, X.; Yang, F.; Wu, C.; Yang, P. Mechanism of nano-anatase TiO2 on promoting photosynthetic carbon reaction of spinach: Inducing complex of rubisco-rubisco activase. Biol. Trace Elem. Res. 2006, 111, 239-253.
Crossref Google Scholar
6

Zheng, L.; Su, M. Y.; Liu, C.; Chen, L.; Huang, H.; Wu, X.; Liu, X. Q.; Yang, F.; Gao, F. Q.; Hong, F. S. Effects of nanoanatase TiO2 on photosynthesis of spinach chloroplasts under different light illumination. Biol. Trace Elem. Res. 2007, 119, 68-76.
Crossref Google Scholar
7

Gao, F. Q.; Liu, C.; Qu, C. X.; Zheng, L.; Yang, F.; Su, M. Y.; Hong, F. S. Was improvement of spinach growth by nano-TiO2 treatment related to the changes of rubisco activase? BioMetals 2008, 21, 211-217.
Crossref Google Scholar
8

Ma, L. L.; Liu, C.; Qu, C. X.; Yin, S. T.; Liu, J.; Gao, F. Q.; Hong, F. H. Rubisco activase mRNA expression in spinach: Modulation by nanoanatase treatment. Biol. Trace Elem. Res. 2008, 122, 168-178.
Crossref Google Scholar
9

Wang, X. M.; Gao, F. Q.; Ma, L. L.; Liu, J.; Yin, S. T.; Yang, P.; Hong, F. S. Effects of nano-anatase on ribulose-1, 5-bisphosphate carboxylase/oxygenase mRNA expression in spinach. Biol. Trace Elem. Res. 2008, 126, 280-289.
Crossref Google Scholar
10

Su, M. Y.; Liu, J.; Yin, S. T.; Ma, L. L.; Hong, F. S. Effects of nanoanatase on the photosynthetic improvement of chloroplast damaged by linolenic acid. Biol. Trace Elem. Res. 2008, 124, 173-183.
Crossref Google Scholar
11

Yang, F.; Hong, F. S.; You, W. J.; Liu, C.; Gao, F. Q.; Wu, C.; Yang, P. Influence of nano-anatase TiO2 on the nitrogen metabolism of growing spinach. Biol. Trace Elem. Res. 2006, 110, 179-190.
Crossref Google Scholar
12

Shah, V.; Belozerova, I. Influence of metal nanoparticles on the soil microbial community and germination of lettuce seeds. Water, Air, Soil Pollut. 2009, 197, 143-148.
Crossref Google Scholar
13

Husen, A.; Siddiqi, K. S. Carbon and fullerene nanomaterials in plant system. J. Nanobiotechnology 2014, 12, 16.
Crossref Google Scholar
14

Qu, G. B.; Bai, Y. H.; Zhang, Y.; Jia, Q.; Zhang, W. D.; Yan, B. The effect of multiwalled carbon nanotube agglomeration on their accumulation in and damage to organs in mice. Carbon 2009, 47, 2060-2069.
Crossref Google Scholar
15

Liu, Q. L.; Chen, B.; Wang, Q. L.; Shi, X. L.; Xiao, Z. Y.; Lin, J. X.; Fang, X. H. Carbon nanotubes as molecular transporters for walled plant cells. Nano Lett. 2009, 9, 1007-1010.
Crossref Google Scholar
16

Kang, S.; Herzberg, M.; Rodrigues, D. F.; Elimelech, M. Antibacterial effects of carbon nanotubes: Size does matter! Langmuir 2008, 24, 6409-6413.
Crossref Google Scholar
17

Tripathi, S.; Sonkar, S. K.; Sarkar, S. Growth stimulation of gram (Cicer arietinum) plant by water soluble carbon nanotubes. Nanoscale 2011, 3, 1176-1181.
Crossref Google Scholar
18

Sonkar, S. K.; Roy, M.; Babar, D. G.; Sarkar, S. Water soluble carbon nano- onions from wood wool as growth promoters for gram plants. Nanoscale 2012, 4, 7670-7675.
Crossref Google Scholar
19

Tripathi, K. M.; Bhati, A., Singh, A.; Sonker, A. K.; Sarkar, S.; Sonkar, S. K. Sustainable changes in the contents of metallic micronutrients in first generation gram seeds imposed by carbon nano-onions: Life cycle seed to seed study. ACS Sustainable Chem. Eng. 2017, 5, 2906-2916.
Crossref Google Scholar
20

Villagarcia, H.; Dervishi, E.; De Silva, K.; Biris, A. S.; Khodakovskaya, M. V. Surface chemistry of carbon nanotubes impacts the growth and expression of water channel protein in tomato plants. Small 2012, 8, 2328-2334.
Crossref Google Scholar
21

Lahiani, M. H.; Dervishi, E.; Chen, J. H.; Nima, Z.; Gaume, A.; Biris, A. S.; Khodakovskaya, M. V. Impact of carbon nanotube exposure to seeds of valuable crops. ACS Appl. Mater. Interfaces 2013, 5, 7965-7973.
Crossref Google Scholar
22

Lahiani, M. H.; Dervishi, E.; Ivanov, I.; Chen, J. H.; Khodakovskaya, M. Comparative study of plant responses to carbon-based nanomaterials with different morphologies. Nanotechnology 2016, 27, 265102.
Crossref Google Scholar
23

Kole, C.; Kole, P.; Randunu, K. M.; Choudhary, P.; Podila, R.; Ke, P. C.; Rao, A. M.; Marcus, R. K. Nanobiotechnology can boost crop production and quality: First evidence from increased plant biomass, fruit yield and phytomedicine content in bitter melon (Momordica charantia). BMC Biotechnol. 2013, 13, 37.
Crossref Google Scholar
24

Saxena, M.; Maity, S.; Sarkar, S. Carbon nanoparticles in "biochar" boost wheat (Triticum aestivum) plant growth. RSC Adv. 2014, 4, 39948-39954.
Crossref Google Scholar
25

Li, H. T.; He, X. D.; Kang, Z. H.; Huang, H.; Liu, Y.; Liu, J. L.; Lian, S. Y.; Tsang, C. H. A.; Yang, X. B.; Lee, S. T. Water-soluble fluorescent carbon quantum dots and photocatalyst design. Angew. Chem., Int. Ed. 2010, 49, 4430-4434.
Crossref Google Scholar
26

Li, H.; Guo, S. J.; Li, C. X.; Huang, H.; Liu, Y.; Kang, Z. H. Tuning laccase catalytic activity with phosphate functionalized carbon dots by visible light. ACS Appl. Mater. Interfaces 2015, 7, 10004-10012.
Crossref Google Scholar
27

Abu-Ghosh, S.; Kumar, V. B.; Fixler, D.; Dubinsky, Z.; Gedanken, A.; Iluz, D. Nitrogen-doped carbon dots prepared from bovine serum albumin to enhance algal astaxanthin production. Algal Res. 2017, 23, 161-165.
Crossref Google Scholar
28

Liu, X. J.; Liu, L. T.; Hu, X. J.; Zhou, S. Y.; Ankri, R.; Fixler, D.; Xie, Z. Multimodal bioimaging based on gold nanorod and carbon dot nanohybrids as a novel tool for atherosclerosis detection. Nano Res. 2018, 11, 1262-1273.
Crossref Google Scholar
29

Niu, Y. F.; Ling, G.; Wang, L.; Guan, S. Y.; Xie, Z.; Barnoy, E. A.; Zhou, S. Y.; Fixler, D. Gold rod-polyethylene glycol-carbon dot nanohybrids as phototheranostic probes. Nanomaterials 2018, 8, 706.
Crossref Google Scholar
30

Guo, S. J.; Zhao, S. Q.; Wu, X. Q.; Li, H.; Zhou, Y. J.; Zhu, C.; Yang, N. J.; Jiang, X.; Gao, J.; Bai, L. et al. A Co3O4-CDots-C3N4 three component electrocatalyst design concept for efficient and tunable CO2 reduction to syngas. Nat. Commun. 2017, 8, 1828.
Crossref Google Scholar
31

Liu, J.; Liu, Y.; Liu, N. Y.; Han, Y. Z.; Zhang, X.; Huang, H.; Lifshitz, Y.; Lee, S. T.; Zhong, J.; Kang, Z. H. Metal-free efficient photocatalyst for stable visible water splitting via a two-electron pathway. Science 2015, 347, 970-974.
Crossref Google Scholar
32

Yu, H.; Du, L. B.; Guan, L. M.; Zhang, K.; Li, Y. Y.; Zhu, H. J.; Sun, M. T.; Wang, S. H. A ratiometric fluorescent probe based on the pi-stacked graphene oxide and cyanine dye for sensitive detection of bisulfite. Sens. Actuators B: Chem. 2017, 247, 823-829.
Crossref Google Scholar
33

Yan, Y. H.; Yu, H.; Zhang, K.; Sun, M. T.; Zhang, Y. J.; Wang, X. K.; Wang, S. H. Dual-emissive nanohybrid of carbon dots and gold nanoclusters for sensitive determination of mercuric ions. Nano Res. 2016, 9, 2088-2096.
Crossref Google Scholar
34

Ge, H. W.; Zhang, K.; Yu, H.; Yue, J.; Yu, L.; Chen, X. F.; Hou, T. X.; Alamry, K. A.; Marwani, H. M.; Wang, S. H. Sensitive and selective detection of antibiotic d-penicillamine based on a dual-mode probe of fluorescent carbon dots and gold nanoparticles. J. Fluoresc. 2018, 28, 1405-1412.
Crossref Google Scholar
35

Li, H.; Kong, W. Q.; Liu, J.; Liu, N. Y.; Huang, H.; Liu, Y.; Kang, Z. H. Fluorescent N-doped carbon dots for both cellular imaging and highly- sensitive catechol detection. Carbon 2015, 91, 66-75.
Crossref Google Scholar
36

Lu, F.; Song, Y. X.; Huang, H.; Liu, Y.; Fu, Y. J.; Huang, J.; Li, H.; Qu, H. H.; Kang, Z. H. Fluorescent carbon dots with tunable negative charges for bio-imaging in bacterial viability assessment. Carbon 2017, 120, 95-102.
Crossref Google Scholar
37

Ge, J. C.; Lan, M. H.; Zhou, B. J.; Liu, W. M.; Guo, L.; Wang, H.; Jia, Q. Y.; Niu, G. L.; Huang, X.; Zhou, H. Y. et al. A graphene quantum dot photodynamic therapy agent with high singlet oxygen generation. Nat. Commun. 2014, 5, 4596.
Crossref Google Scholar
38

Lim, S. Y.; Shen, W.; Gao, Z. Q. Carbon quantum dots and their applications. Chem. Soc. Rev. 2015, 44, 362-381.
Crossref Google Scholar
39

Jermyn, M. A. Increasing the sensitivity of the anthrone method for carbohydrate. Anal. Biochem. 1975, 68, 332-335.
Crossref Google Scholar
40

Loewus, F. A. Improvement in anthrone method for determination of carbohydrates. Anal. Chem. 1952, 24, 219.
Crossref Google Scholar
41

Hohl, H.; Stumm, W. Interaction of Pb2+ with hydrous γ-Al2O3. J. Colloid Interface Sci. 1976, 55, 281-288.
Crossref Google Scholar
42

Crocker, M.; Herold, R. H. M.; Sonnemans, M. H. W.; Emeis, C. A.; Wilson, A. E.; Van Der Moolen, J. N. Studies on the acidity of mordenite and ZSM 5. 1. Determination of broensted acid site concentrations in mordenite and ZSM 5 by conductometric titration. J. Phys. Chem. 1993, 97, 432-439.
Crossref Google Scholar
43

Hunt, J. P.; Taube, H. The photochemical decomposition of hydrogen peroxide. Quantum yields, tracer and fractionation effects. J. Am. Chem. Soc. 1952, 74, 5999-6002.
Crossref Google Scholar
44

Zhao, Y.; Allen, B. L.; Star, A. Enzymatic degradation of multiwalled carbon nanotubes. J. Phys. Chem. A 2011, 115, 9536-9544.
Crossref Google Scholar
45

Lan, Y.; Mott, K. A. Determination of apparent Km values for ribulose 1, 5-bisphosphate carboxylase/oxygenase (Rubisco) activase using the spectrophotometric assay of rubisco activity. Plant Physiol. 1991, 95, 604-609.
Crossref Google Scholar
46

Chong, Y.; Ma, Y. F.; Shen, H.; Tu, X. L.; Zhou, X.; Xu, J. Y.; Dai, J. W.; Fan, S. J.; Zhang, Z. J. The in vitro and in vivo toxicity of graphene quantum dots. Biomaterials 2014, 35, 5041-5048.
Crossref Google Scholar
47

Ming, H.; Ma, Z.; Liu, Y.; Pan, K. M.; Yu, H.; Wang, F.; Kang, Z. H. Large scale electrochemical synthesis of high quality carbon nanodots and their photocatalytic property. Dalton Trans. 2012, 41, 9526-9531.
Crossref Google Scholar
48

Li, H.; Zhang, M. L.; Song, Y. X.; Wang, H. B.; Liu, C. A.; Fu, Y. J.; Huang, H.; Liu, Y.; Kang, Z. H. Multifunctional carbon dot for lifetime thermal sensing, nucleolus imaging and antialgal activity. J. Mater. Chem. B 2018, 6, 5708-5717.
Crossref Google Scholar
49

Khodakovskaya, M.; Dervishi, E.; Mahmood, M.; Xu, Y.; Li, Z. R.; Watanabe, F.; Biris, A. S. Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano 2009, 3, 3221-3227.
Crossref Google Scholar
50

Wilson, A. T.; Calvin, M. The Photosynthetic cycle. CO2 dependent transients. J. Am. Chem. Soc. 1955, 77, 5948-5957.
Crossref Google Scholar
51

Walker, D. A. Three phases of chloroplast research. Nature 1970, 226, 1204- 1208.
Crossref Google Scholar
52

Lin, M. T.; Occhialini, A.; Andralojc, P. J.; Parry, M. A. J.; Hanson, M. R. A faster rubisco with potential to increase photosynthesis in crops. Nature 2014, 513, 547-550.
Crossref Google Scholar
53

Wong, S. W.; Cowan, I. R.; Farquhar, G. D. Stomatal conductance correlates with photosynthetic capacity. Nature 1979, 282, 424-426.
Crossref Google Scholar
54

Kotchey, G. P.; Hasan, S. A.; Kapralov, A. A.; Ha, S. H.; Kim, K.; Shvedova, A. A.; Kagan, V. E.; Star, A. A natural vanishing act: The enzyme-catalyzed degradation of carbon nanomaterials. Acc. Chem. Res. 2012, 45, 1770-1781.
Crossref Google Scholar
55

Allen, B. L.; Kotchey, G. P.; Chen, Y. N.; Yanamala, N. V. K.; Klein- Seetharaman, J.; Kagan, V. E.; Star, A. Mechanistic investigations of horseradish peroxidase-catalyzed degradation of single-walled carbon nanotubes. J. Am. Chem. Soc. 2009, 131, 17194-17205.
Crossref Google Scholar
56

Went, F. W.; Thimann, K. V. Phytohormones; The Macmillan Company: New York, 1937.
Google Scholar
57

Nurunnabi, M.; Khatun, Z.; Huh, K. M.; Park, S. Y.; Lee, D. Y.; Cho, K. J.; Lee, Y. K. In vivo biodistribution and toxicology of carboxylated graphene quantum dots. ACS Nano 2013, 7, 6858-6867.
Crossref Google Scholar
Nano Research
Volume 12 Issue 7,
July 2019
Pages 1585-1593
DOI: 10.1007/s12274-019-2397-5
Cite this article:
Li H, Huang J, Liu Y, et al. Enhanced RuBisCO activity and promoted dicotyledons growth with degradable carbon dots. Nano Research, 2019, 12(7): 1585-1593. https://doi.org/10.1007/s12274-019-2397-5
Topics:
CarbonMetal ionsMetalsPlants (botany)

1097

Views

88

Crossref

N/A

Web of Science

92

Scopus

10

CSCD

Altmetrics
Received: 27 November 2018
Revised: 30 March 2019
Accepted: 01 April 2019
Published: 15 April 2019
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019
Return