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Research Article

3D printing of living bacteria electrode

Megan C. Freyman§, Tianyi Kou§, Shanwen Wang, Yat Li( )
Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA

§Megan C. Freyman and Tianyi Kou contributed equally to this work.

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Abstract

The capability of preparing three-dimensional (3D) printable living inks provides a unique way to harness the activity of microbes and use them in functional devices. Here we demonstrate the incorporation of the living bacteria Shewanella Oneidensis MR-1 (S. Oneidensis MR-1) directly into an ink used for creating 3D printed structures. Significantly, S. Oneidensis MR-1 survives the 3D printing process by showing prominent activity in degrading the methyl orange azo dye. Through the addition of carbon black to this ink, we further demonstrate the direct printing of a living microbial fuel cell (MFC) anode. To our knowledge, this is the first report on implementing 3D printed bacteria structure as a living electrode for an MFC system. The capability of printing living and functional 3D bacterial structure could open up new possibilities in design and fabrication of microbial devices as well as fundamental research on the interaction between different bacterial strains, electrode materials, and surrounding environments.

Keywords

Shewanella Oneidensis MR-1microbial fuel cellsthree-dimensional (3D) printingbacteria inkliving electrode

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Nano Research
Volume 13 Issue 5,
May 2020
Pages 1318-1323
DOI: 10.1007/s12274-019-2534-1
Cite this article:
Freyman MC, Kou T, Wang S, et al. 3D printing of living bacteria electrode. Nano Research, 2020, 13(5): 1318-1323. https://doi.org/10.1007/s12274-019-2534-1
Topics:
AnodesBacteria Carbon blackMicrobial fuel cells

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Received: 29 August 2019
Revised: 03 October 2019
Accepted: 04 October 2019
Published: 23 October 2019
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019
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