AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Research Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article

3D printing of living bacteria electrode

Megan C. Freyman§, Tianyi Kou§, Shanwen Wang, Yat Li( )
Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA

§Megan C. Freyman and Tianyi Kou contributed equally to this work.

Show Author Information

Graphical Abstract

Abstract

The capability of preparing three-dimensional (3D) printable living inks provides a unique way to harness the activity of microbes and use them in functional devices. Here we demonstrate the incorporation of the living bacteria Shewanella Oneidensis MR-1 (S. Oneidensis MR-1) directly into an ink used for creating 3D printed structures. Significantly, S. Oneidensis MR-1 survives the 3D printing process by showing prominent activity in degrading the methyl orange azo dye. Through the addition of carbon black to this ink, we further demonstrate the direct printing of a living microbial fuel cell (MFC) anode. To our knowledge, this is the first report on implementing 3D printed bacteria structure as a living electrode for an MFC system. The capability of printing living and functional 3D bacterial structure could open up new possibilities in design and fabrication of microbial devices as well as fundamental research on the interaction between different bacterial strains, electrode materials, and surrounding environments.

Keywords

Shewanella Oneidensis MR-1microbial fuel cellsthree-dimensional (3D) printingbacteria inkliving electrode

Electronic Supplementary Material

Download File(s)
12274_2019_2534_MOESM1_ESM.pdf (2 MB)

References

[1]
Eş, I.; Mousavi Khaneghah, A.; Barba, F. J.; Saraiva, J. A.; Sant’Ana, A. S.; Hashemi, S. M. B. Recent advancements in lactic acid production—A review. Food Res. Int. 2018, 107, 763-770.
Crossref Google Scholar
[2]
Cantera, S.; Muñoz, R.; Lebrero, R.; López, J. C.; Rodríguez, Y.; García-Encina, P. A. Technologies for the bioconversion of methane into more valuable products. Curr. Opin. Biotechnol. 2018, 50, 128-135.
Crossref Google Scholar
[3]
Liamleam, W.; Annachhatre, A. P. Electron donors for biological sulfate reduction. Biotechnol. Adv. 2007, 25, 452-463.
Crossref Google Scholar
[4]
Kolmert, Å.; Johnson, D. B. Remediation of acidic waste waters using immobilised, acidophilic sulfate-reducing bacteria. J. Chem. Technol. Biotechnol. 2001, 76, 836-843.
Crossref Google Scholar
[5]
Lovley, D. R.; Phillips, E. J. P.; Gorby, Y. A.; Landa, E. R. Microbial reduction of uranium. Nature 1991, 350, 413-416.
Crossref Google Scholar
[6]
Golitsch, F.; Bücking, C.; Gescher, J. Proof of principle for an engineered microbial biosensor based on Shewanella oneidensis outer membrane protein complexes. Biosens. Bioelectron. 2013, 47, 285-291.
Crossref Google Scholar
[7]
Wang, G. M.; Qian, F.; Saltikov, C. W.; Jiao, Y. Q.; Li, Y. Microbial reduction of graphene oxide by Shewanella. Nano Res. 2011, 4, 563-570.
Crossref Google Scholar
[8]
Qian, F.; Wang, H. Y.; Ling, Y. C.; Wang, G. M.; Thelen, M. P.; Li, Y. Photoenhanced electrochemical interaction between Shewanella and a hematite nanowire photoanode. Nano Lett. 2014, 14, 3688-3693.
Crossref Google Scholar
[9]
Wang, H. Y.; Qian, F.; Li, Y. Solar-assisted microbial fuel cells for bioelectricity and chemical fuel generation. Nano Energy 2014, 8, 264-273.
Crossref Google Scholar
[10]
Wang, H. Y.; Qian, F.; Wang, G. M.; Jiao, Y. Q.; He, Z.; Li, Y. Self-biased solar-microbial device for sustainable hydrogen generation. ACS Nano 2013, 7, 8728-8735.
Crossref Google Scholar
[11]
Qian, F.; Wang, G. M.; Li, Y. Solar-driven microbial photoelectrochemical cells with a nanowire photocathode. Nano Lett. 2010, 10, 4686-4691.
Crossref Google Scholar
[12]
Lovley, D. R. Electromicrobiology. Annu. Rev. Microbiol. 2012, 66, 391-409.
Crossref Google Scholar
[13]
Wang, H. Y.; Wang, G. M.; Ling, Y. C.; Qian, F.; Song, Y.; Lu, X. H.; Chen, S. W.; Tong, Y. X.; Li, Y. High power density microbial fuel cell with flexible 3D graphene-nickel foam as anode. Nanoscale 2013, 5, 10283-10290.
Crossref Google Scholar
[14]
Lehner, B. A. E.; Schmieden, D. T.; Meyer, A. S. A straightforward approach for 3D bacterial printing. ACS Synth. Biol. 2017, 6, 1124-1130.
Crossref Google Scholar
[15]
Qian, F.; Zhu, C.; Knipe, J. M.; Ruelas, S.; Stolaroff, J. K.; DeOtte, J. R.; Duoss, E. B.; Spadaccini, C. M.; Henard, C. A.; Guarnieri, M. T. et al. Direct writing of tunable living inks for bioprocess intensification. Nano Lett. 2019, 19, 5829-5835.
Crossref Google Scholar
[16]
Kuo, C. K.; Ma, P. X. Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties. Biomaterials 2001, 22, 511-521.
Crossref Google Scholar
[17]
LIVE/DEAD® BacLightTM Bacterial Viability Kits. 2004.
Crossref
[18]
Cai, P. J.; Xiao, X.; He, Y. R.; Li, W. W.; Chu, J.; Wu, C.; He, M. X.; Zhang, Z.; Sheng, G. P.; Lam, M. H. W. et al. Anaerobic biodecolorization mechanism of methyl orange by Shewanella oneidensis MR-1. Appl. Microbiol. Biotechnol. 2012, 93, 1769-1776.
Crossref Google Scholar
[19]
Xiao, L.; Damien, J.; Luo, J. Y.; Jang, H. D.; Huang, J. X.; He, Z. Crumpled graphene particles for microbial fuel cell electrodes. J. Power Sources 2012, 208, 187-192.
Crossref Google Scholar
[20]
Hou, H. J.; Li, L.; Ceylan, C. Ü.; Haynes, A.; Cope, J.; Wilkinson, H. H.; Erbay, C.; de Figueiredo, P.; Han, A. A microfluidic microbial fuel cell array that supports long-term multiplexed analyses of electricigens. Lab Chip 2012, 12, 4151-4159.
Crossref Google Scholar
[21]
Yang, Y.; Liu, T. Y.; Zhu, X.; Zhang, F.; Ye, D. D.; Liao, Q.; Li, Y. Boosting power density of microbial fuel cells with 3D nitrogen-doped graphene aerogel electrode. Adv. Sci. 2016, 3, 1600097.
Crossref Google Scholar
[22]
Jiang, X. C.; Hu, J. S.; Fitzgerald, L. A.; Biffinger, J. C.; Xie, P.; Ringeisen, B. R.; Lieber, C. M. Probing electron transfer mechanisms in Shewanella oneidensis MR-1 using a nanoelectrode platform and single-cell imaging. Proc. Natl. Acad. Sci. USA 2010, 107, 16806-16810.
Crossref Google Scholar
[23]
Baron, D.; LaBelle, E.; Coursolle, D.; Gralnick, J. A.; Bond, D. R. Electrochemical measurement of electron transfer kinetics by Shewanella oneidensis MR-1. J. Biol. Chem. 2009, 284, 28865-28873.
Google Scholar
Nano Research
Volume 13 Issue 5,
May 2020
Pages 1318-1323
DOI: 10.1007/s12274-019-2534-1
Cite this article:
Freyman MC, Kou T, Wang S, et al. 3D printing of living bacteria electrode. Nano Research, 2020, 13(5): 1318-1323. https://doi.org/10.1007/s12274-019-2534-1
Topics:
AnodesBacteria Carbon blackMicrobial fuel cells

1065

Views

59

Crossref

N/A

Web of Science

56

Scopus

4

CSCD

Altmetrics
Received: 29 August 2019
Revised: 03 October 2019
Accepted: 04 October 2019
Published: 23 October 2019
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019
Return