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Research Article

Mimicking peroxidase active site microenvironment by functionalized graphene quantum dots

Qi Xin1,§, Xinrui Jia1,2,§, Asmat Nawaz1,2, Wenjing Xie1, Litao Li3, Jian Ru Gong1,2( )
CAS Center of Excellence for Nanoscience, CAS Key Laboratory for Nanosystem & Hierarchical Fabrication, National Center for Nanoscience and Technology, 11 Beiyitiao Zhongguancun, Beijing 100190, China
University of Chinese Academy of Sciences, Beijing 100049, China
Department of Orthopaedics, the 309th Hospital of the PLA, Beijing 100091, China

§ Qi Xin and Xinrui Jia contributed equally to this work.

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Abstract

The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes. From the perspective of mimicking active site microenvironment at low cost, we herein report a novel histidine-functionalized graphene quantum dot (His-GQD)/hemin complex, which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so far. Also, our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature range. Lineweaver-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this mimetic. In addition, His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood glucose. Our work provides an effective design of artificial enzymes for practical applications.

Keywords

nanoenzymegraphene quantum dotheme active siteperoxidase mimeticenzymatic microenvironment

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Nano Research
Volume 13 Issue 5,
May 2020
Pages 1427-1433
DOI: 10.1007/s12274-020-2678-z
Cite this article:
Xin Q, Jia X, Nawaz A, et al. Mimicking peroxidase active site microenvironment by functionalized graphene quantum dots. Nano Research, 2020, 13(5): 1427-1433. https://doi.org/10.1007/s12274-020-2678-z
Topics:
Carbon quantum dotsEnzymesGrapheneNanocrystalsSemiconductor quantum dots

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Received: 16 November 2019
Revised: 05 January 2020
Accepted: 26 January 2020
Published: 20 February 2020
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
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