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Research Article

Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants

Xinzhou Wu1,2,§, Run Qin1,2,§, Hanxiang Wu1,2, Guangkai Yao1,2, Yue Zhang1,2, Ping Li1,2, Yizhu Xu1,2, Zhixiang Zhang1,2, Zhibin Yin3( ), Hanhong Xu1,2( )
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China

§ Xinzhou Wu and Run Qin contributed equally to this work.

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Abstract

Design and discovery of carrier-mediated modified pesticides are vital for reducing pesticide dosage and increasing utilization, yet it remains a great challenge due to limited insights into plant translocation mechanisms. Nanostructure/nanoparticle assisted laser desorption/ionization strategy has established itself as a preferential analytical tool for biological tissue analysis, whereas potential applications in plant sciences are hindered with regard to the inability to slice plant leaves and petals. Herein, we report gold nanoparticle (AuNP)-immersed paper imprinting mass spectrometry imaging (MSI) for the spatiotemporal visualization of pesticide translocation in plant leaves. This approach plays a dual role in preserving spatial information and improving ionization efficiency for pesticides regardless of imaging artifacts due to homogenous AuNP deposition. Using this MSI platform, we proposed the elaborate plant translocation mechanism of agrochemicals for the first time, which is currently poorly understood. The dynamic processes of carrier-mediated pesticides can be clearly visualized, including crossing of plasma membranes by transporters, translocation downward in stems through the phloem, diffusion to the xylem and, conversely, accumulation at margins of the treated leaves. Moreover, this AuNP-assisted paper imprinting method could be highly compatible with laser-based MSI instruments, expediting researches across a broad range of fields, especially in nanomaterial development and life sciences.

Keywords

gold nanoparticlesnanoparticle-immersed paper imprintingmass spectrometry imagingplant translocation mechanismcarrier-mediated pesticides

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Nano Research
Volume 13 Issue 3,
March 2020
Pages 611-620
DOI: 10.1007/s12274-020-2700-5
Cite this article:
Wu X, Qin R, Wu H, et al. Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants. Nano Research, 2020, 13(3): 611-620. https://doi.org/10.1007/s12274-020-2700-5
Topics:
Cell membranes Gold nanoparticlesMass spectrometryMetal nanoparticles

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Received: 11 January 2020
Revised: 01 February 2020
Accepted: 05 February 2020
Published: 28 February 2020
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
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