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Research Article

Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity

Marianna Barbalinardo1,§, Andrea Antosova2,§, Marta Gambucci3,§, Zuzana Bednarikova2, Cristiano Albonetti1, Francesco Valle1,4, Paola Sassi3, Loredana Latterini3( ), Zuzana Gazova2,5, Eva Bystrenova1( )
C.N.R. - I.S.M.N, via Gobetti, 101, Bologna 40129, Italy
Institute of Experimental Physics, Department of Biophysics, Slovak Academy of Science, Watsonova 47 04001, Kosice, Slovakia
Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia 06123, Italy
Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase (CSGI), ISMN-CNR, Bologna 40129, Italy
Department of Medical and Clinical Biochemistry, Faculty of Medicine, Safarik University, Kosice 04001, Slovakia

§ Marianna Barbalinardo, Andrea Antosova, and Marta Gambucci contributed equally to this work.

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Abstract

The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases. The influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of nanoparticles. Various physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution, thus influencing the disaggregation of preformed fibrils. We have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells (SH-SY5Y). In particular, fluorescence, infrared and circular dichroism spectroscopies, optical and atomic force microscopy experiments have been carried out; the results are analyzed to rationalize the effects of these complexes on neural cell viability. It is remarkable, that the fibrils in the presence of AuNPs, unlike fibrils alone or with AgNPs, do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.

Keywords

lysozymeamyloid fibrilsnanoparticlesspectroscopytoxicity

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Nano Research
Volume 13 Issue 4,
April 2020
Pages 1081-1089
DOI: 10.1007/s12274-020-2748-2
Cite this article:
Barbalinardo M, Antosova A, Gambucci M, et al. Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity. Nano Research, 2020, 13(4): 1081-1089. https://doi.org/10.1007/s12274-020-2748-2
Topics:
CellsCircular dichroism spectroscopyGlycoproteinsNanoparticles

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Received: 28 November 2019
Revised: 14 February 2020
Accepted: 10 March 2020
Published: 14 April 2020
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
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