Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays

Jingyu Xie; Yilin Song; Yuchuan Dai; Ziyue Li; Fei Gao; Xuanyu Li; Guihua Xiao; Yu Zhang; Hao Wang; Zeying Lu; Xingyu Jiang; Wenfu Zheng; Xinxia Cai

doi:10.1007/s12274-020-2802-0

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Research Article

Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays

Jingyu Xie^{¹^,²}, Yilin Song^{¹^,²}, Yuchuan Dai^{¹^,²}, Ziyue Li^{¹^,²}, Fei Gao^{¹^,²}, Xuanyu Li^{²^,³}, Guihua Xiao^{¹^,²}, Yu Zhang^{¹^,²}, Hao Wang^{¹^,²}, Zeying Lu^{¹^,²}, Xingyu Jiang^{³^,⁴}, Wenfu Zheng^³, Xinxia Cai^{¹^,²}(

)

1State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China

2University of Chinese Academy of Sciences, Beijing 100049, China

3Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China

4Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China

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Graphical Abstract

Abstract

Effective and precise neural modulation with real-time detection in the brain is of great importance and represents a significant challenge. Nanoliposome-encapsulated light-sensitive compounds have excellent characteristics such as high temporal and spatial resolution, delayed drug clearance, and restricted drug biodistribution for neural modulation. In this study, we developed a nanoliposome-based delivery system for ruthenium-based caged GABA compounds (Nanolipo-Ru) to modulate neural activity and allow for real-time monitoring using the microelectrode arrays (MEAs). The Nanolipo-Ru nanoparticles had an average size of 134.10 ± 4.30 nm and exhibited excellent stability for seven weeks. For the in vivo experiment in the rat, release of GABA by Nanolipo-Ru under blue light illumination resulted in an average firing rate reduction in interneurons and pyramidal neurons in the same brain region of 79.4% and 81.6%, respectively. Simultaneously, the average power of local field potentials in the 0-15 Hz range degraded from 4.34 to 0.85 mW. In addition, the Nanolipo-Ru nanoparticles have the potential to provide more flexible timing of modulation than unencapsulated RuBi-GABA in the experiments. These results indicated that Nanolipo-Ru could be an effective platform for regulating neuronal electrophysiology. Furthermore, nanoliposomes with appropriate modifications would render promising utilities for targeting of specific types of neurons in the future.

Keywords

neural modulation nanoliposome microelectrode array light-sensitive drug

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Nano Research

Volume 13 Issue 6,
June 2020

Pages 1756-1763

DOI: 10.1007/s12274-020-2802-0

Cite this article:

Xie J, Song Y, Dai Y, et al. Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays. Nano Research, 2020, 13(6): 1756-1763. https://doi.org/10.1007/s12274-020-2802-0

Topics:

Brain Controlled drug delivery Electrophysiology Nanoparticles Neurons Targeted drug delivery

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Received: 16 December 2019

Revised: 04 April 2020

Accepted: 17 April 2020

Published: 11 May 2020