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Research Article

Multiplexed intracellular detection based on dual-excitation/dual-emission upconversion nanoprobes

Jianxi Ke1,2,3, Shan Lu1,3,4( ), Zhuo Li1, Xiaoying Shang1, Xingjun Li1, Renfu Li1, Datao Tu1,4, Zhuo Chen1, Xueyuan Chen1,2,3,4( )
CAS Key Laboratory of Design and Assembly of Functional Nanostructures, and Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China
School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China
University of Chinese Academy of Sciences, Beijing 100049, China
Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou 350108, China
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Graphical Abstract

Abstract

Multiplexed intracellular detection is desirable in biomedical sciences for its higher efficiency and accuracy compared to the single-analyte detection. However, it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously. Herein, we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles (UCNP) loaded with two dyes for sensitization and quenching of the upconversion luminescence (UCL), respectively. Based on the two independent energy transfer processes of near-infrared (NIR) dye IR845 to UCNP and UCNP to visible dye PAPS-Zn, ClO- and Zn2+ were simultaneously detected with a limit of detection (LOD) of 41.4 and 10.5 nM, respectively. By utilizing a purpose-built 830/980 nm dual-laser confocal microscope, both intrinsic and exogenous ClO- and Zn2+ in live MCF-7 cells have been accurately quantified. Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells. Through modulation of NIR dye and visible dye with other reactive groups, the nanoprobes can be extended to analyze various intracellular species, which provides a promising tool to study the biological activities in live cells and diagnose diseases.

Keywords

upconversion nanoparticlesenergy transferdual-excitationdye sensitizationratiometric probemultiplexed detection

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Nano Research
Volume 13 Issue 7,
July 2020
Pages 1955-1961
DOI: 10.1007/s12274-020-2837-2
Cite this article:
Ke J, Lu S, Li Z, et al. Multiplexed intracellular detection based on dual-excitation/dual-emission upconversion nanoprobes. Nano Research, 2020, 13(7): 1955-1961. https://doi.org/10.1007/s12274-020-2837-2
Topics:
Chlorine compoundsInfrared devices NanoprobesSignal processing
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Sixth Nano Research Award

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Received: 15 January 2020
Revised: 24 April 2020
Accepted: 28 April 2020
Published: 22 May 2020
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
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