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Research Article

Demonstration of biophoton-driven DNA replication via gold nanoparticle-distance modulated yield oscillation

Na Li1,2,§, Daoling Peng3,§, Xianjing Zhang1,2, Yousheng Shu4, Feng Zhang1,2,5( ), Lei Jiang6,7,8( ), Bo Song1( )
School of Optical-Electrical Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
State Key Laboratory of Respiratory Disease, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital, Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, China
School of Chemistry, South China Normal University, Guangzhou 510006, China
State Key Laboratory of Medical Neurobiology, Institute for Translational Brain Research, MOE Frontiers Center for Brain Science, Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200032, China
Biomedical Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010011, China
Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China
Key Laboratory of Bio-inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry, Beihang University, Beijing 100191, China

§ Na Li and Daoling Peng contributed equally to this work.

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Abstract

Biologically, there exist two kinds of syntheses: photosynthesis and ATP-driven biosynthesis. The light harvesting of photosynthesis is known to achieve an efficiency of ~ 95% by the quantum energy transfer of photons. However, how the ATP-driven biosynthesis reaches its high efficiency still remains unknown. Deoxynucleotide triphosphates (dNTPs) in polymerase chain reaction (PCR) adopt the identical way of ATP to release their energy, and thus can be employed to explore the ATP energy process. Here, using a gold nanoparticle (AuNP) enhanced PCR (AuNP-PCR), we demonstrate that the energy released by phosphoanhydride-bond (PB) hydrolysis of dNTPs is in form of photons (PB-photons) to drive DNA replication, by modulating their resonance with the average inter-AuNP distance (D). The experimental results show that both the efficiency and yield of PCR periodically oscillate with D increasing, indicating a quantized process, but not simply a thermal one. The PB-photon wavelength is further determined to 8.4 μm. All these results support that the release, transfer and utilization of bioenergy are in the form of photons. Our findings of ATP-energy quantum conversion will open a new avenue to the studies of high-efficiency bioenergy utilization, biochemistry, biological quantum physics, and even brain sciences.

Keywords

intrinsic biophotonDNA replicationpolymerase chain reaction (PCR)gold nanoparticlehigh-efficiency energy utilizationbiological quantum physics

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Nano Research
Volume 14 Issue 1,
January 2021
Pages 40-45
DOI: 10.1007/s12274-020-2937-z
Cite this article:
Li N, Peng D, Zhang X, et al. Demonstration of biophoton-driven DNA replication via gold nanoparticle-distance modulated yield oscillation. Nano Research, 2021, 14(1): 40-45. https://doi.org/10.1007/s12274-020-2937-z
Topics:
Biochemistry BiosynthesisGold nanoparticlesMetal nanoparticlesPhotons

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Received: 08 June 2020
Accepted: 13 June 2020
Published: 05 January 2021
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature
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