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Research Article

2-D/2-D heterostructured biomimetic enzyme by interfacial assembling Mn3(PO4)2 and MXene as a flexible platform for real- time sensitive sensing cell superoxide

Shen Fei Zhao1,§, Fang Xin Hu1,§, Zhuan Zhuan Shi1, Jing Jing Fu2, Yue Chen2, Fang Yin Dai3, Chun Xian Guo1( ), Chang Ming Li1,2,4( )
Institute of Materials Science and Devices, Suzhou University of Science and Technology, Suzhou 215009, China
Institute for Clean Energy and Advanced Materials, Southwest University, Chongqing 400715, China
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
Institute for Advanced Cross-field Science & College of Life Science, Qingdao University, Qingdao 200671, China

§ Shen Fei Zhao and Fang Xin Hu contributed equally to this work.

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Abstract

It is critical for fabricating flexible biosensors with both high sensitivity and good selectivity to realize real-time monitoring superoxide anion (O2•−), a specific reactive oxygen species that plays critical roles in various biological processes. This work delicately designs a Mn3(PO4)2/MXene heterostructured biomimetic enzyme by assembling two-dimensional (2-D) Mn3(PO4)2 nanosheets with biomimetic activity and 2-D MXene nanosheets with high conductivity and abundant functional groups. The 2-D nature of the two components with strong interfacial interaction synergistically enables the heterostructure an excellent flexibility with retained 100% of the response when to reach a bending angle up to 180°, and 96% of the response after 100 bending/relaxing cycles. It is found that the surface charge state of the heterostructure promotes the adsorption of O2•−, while the high-energy active site improves electrochemical oxidation of O2•−. The Mn3(PO4)2/MXene as a sensing platform towards O2•− achieves a high sensitivity of 64.93 μA·μM−1·cm−2, a wide detection range of 5.75 nM to 25.93 μM, and a low detection limit of 1.63 nM. Finally, the flexible heterostructured sensing platform realizes real-time monitoring of O2•− in live cell assays, offering a promising flexible biosensor towards exploring various biological processes.

Keywords

two-dimensional (2-D) nanosheetsassembled heterostructureinterfaceflexible biosensorreal-time monitoring

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Nano Research
Volume 14 Issue 3,
March 2021
Pages 879-886
DOI: 10.1007/s12274-020-3130-0
Cite this article:
Zhao SF, Hu FX, Shi ZZ, et al. 2-D/2-D heterostructured biomimetic enzyme by interfacial assembling Mn3(PO4)2 and MXene as a flexible platform for real- time sensitive sensing cell superoxide. Nano Research, 2021, 14(3): 879-886. https://doi.org/10.1007/s12274-020-3130-0
Topics:
Biomimetics EnzymesNanosheets

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Received: 16 July 2020
Revised: 17 September 2020
Accepted: 20 September 2020
Published: 01 March 2021
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature
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