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Research Article

In situ self-assembly of near-infrared-emitting gold nanoparticles into body-clearable 1D nanostructures with rapid lysosome escape and fast cellular excretion

Kui He, Jiayi Zhu, Lingshan Gong, Yue Tan, Huarui Chen, Huarun Liang, Baihao Huang, Jinbin Liu( )
Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China
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Abstract

The integration of strong near-infrared (NIR) emission, rapid lysosome escape, fast cellular excretion, and efficient total body clearance is highly desired for nanoparticles (NPs) to achieve synergistic functions in both molecular imaging and delivery. Herein, using a well-designed cyclopeptide (CP) that can spontaneously assemble into controllable nanofibers as template, a facile strategy is reported for in situ self-assembly of NIR-emitting gold NPs (AuNPs) into ordered and well-controlled one-dimensional (1D) nanostructures (AuNPs@CP) with greatly enhanced NIR emission (~ 6 fold). Comparing with the unassembled AuNPs, the AuNPs@CP are observed to enter living cells through endocytosis, escape from lysosome rapidly, and excrete the cell fast, which shows high gene transfection efficiencies in construction of cell line with ~ 7.5-fold overexpression of p53 protein. Furthermore, the AuNPs@CP exhibit high in vivo diffusibility and total body clearance efficiency with minimized healthy organ retention, which are also demonstrated to be good nanovectors for plasmid complementary deoxyribonucleic acid 3.1 (pcDNA3.1)(+)-internal ribosome entry site (IRES)-green fluorescent protein (GFP)-p53 plasmid with efficient p53 gene over-expression in tumor site. This facile in situ strategy in fabricating highly luminescent 1D nanostructures provides a promising approach toward future translatable multifunctional nanostructures for delivering, tracking, and therapy.

Keywords

luminescent gold nanoparticleself-assemblyintracellular imagingbody clearancegene delivery

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References

[1]
S. E. Crawford,; M. J. Hartmann,; J. E. Millstone, Surface chemistry- mediated near-infrared emission of small coinage metal nanoparticles. Acc. Chem. Res. 2019, 52, 695-703.
Crossref Google Scholar
[2]
Z. N. Wu,; Y. H. Du,; J. L. Liu,; Q. F. Yao,; T. K. Chen,; Y. T. Cao,; H. Zhang,; J. P. Xie, Aurophilic interactions in the self-assembly of gold nanoclusters into nanoribbons with enhanced luminescence. Angew. Chem., Int. Ed. 2019, 58, 8139-8144.
Crossref Google Scholar
[3]
L. S. Gong,; Y. Chen,; K. He,; J. B. Liu, Surface coverage-regulated cellular interaction of ultrasmall luminescent gold nanoparticles. ACS Nano 2019, 13, 1893-1899.
Crossref Google Scholar
[4]
K. Y. Zheng,; M. I. Setyawati,; D. T. Leong,; J. P. Xie, Surface ligand chemistry of gold nanoclusters determines their antimicrobial ability. Chem. Mater. 2018, 30, 2800-2808.
Crossref Google Scholar
[5]
M. X. Yu,; J. Xu,; J. Zheng, Renal clearable luminescent gold nanoparticles: From the bench to the clinic. Angew. Chem., Int. Ed. 2019, 58, 4112-4128.
Crossref Google Scholar
[6]
L. Li,; Z. Yang,; W. P. Fan,; L. C. He,; C. Cui,; J. H. Zou,; W. Tang,; O. Jacobson,; Z. T. Wang,; G. Niu, et al. In situ polymerized hollow mesoporous organosilica biocatalysis nanoreactor for enhancing ROS-mediated anticancer therapy. Adv. Funct. Mater. 2020, 30, 1907716.
Crossref Google Scholar
[7]
L. W. Liao,; S. L. Zhuang,; P. Wang,; Y. N. Xu,; N. Yan,; H. W. Dong,; C. M. Wang,; Y. Zhao,; N. Xia,; J. Li, et al. Quasi-dual-packed- kerneled Au49(2,4-DMBT)27 nanoclusters and the influence of kernel packing on the electrochemical gap. Angew. Chem., Int. Ed. 2017, 56, 12644-12648.
Crossref Google Scholar
[8]
D. Y. Chen,; Z. T. Luo,; N. J. Li,; J. Y. Lee,; J. P. Xie,; J. M. Lu, Amphiphilic polymeric nanocarriers with luminescent gold nanoclusters for concurrent bioimaging and controlled drug release. Adv. Funct. Mater. 2013, 23, 4324-4331.
Crossref Google Scholar
[9]
X. Y. Jiang,; B. J. Du,; J. Zheng, Glutathione-mediated biotransformation in the liver modulates nanoparticle transport. Nat. Nanotechnol. 2019, 14, 874-882.
Crossref Google Scholar
[10]
Y. F. Lei,; L. X. Tang,; Y. Z. Y. Xie,; Y. L. Xianyu,; L. M. Zhang,; P. Wang,; Y. Hamada,; K. Jiang,; W. F. Zheng,; X. Y. Jiang, Gold nanoclusters-assisted delivery of NGF siRNA for effective treatment of pancreatic cancer. Nat. Commun. 2017, 8, 15130.
Crossref Google Scholar
[11]
Y. Q. Sun,; D. D. Wang,; Y. Q. Zhao,; T. X. Zhao,; H. C. Sun,; X. W. Li,; C. X. Wang,; B. Yang,; Q. Lin, Polycation-functionalized gold nanodots with tunable near-infrared fluorescence for simultaneous gene delivery and cell imaging. Nano Res. 2018, 11, 2392-2404.
Crossref Google Scholar
[12]
Q. Z. Li,; Y. T. Pan,; T. K. Chen,; Y. X. Du,; H. H. Ge,; B. C. Zhang,; J. P. Xie,; H. Z. Yu,; M. Z. Zhu, Design and mechanistic study of a novel gold nanocluster-based drug delivery system. Nanoscale 2018, 10, 10166-10172.
Crossref Google Scholar
[13]
A. Yahia-Ammar,; D. Sierra,; F. Mérola,; N. Hildebrandt,; X. Le Guével, Self-assembled gold nanoclusters for bright fluorescence imaging and enhanced drug delivery. ACS Nano 2016, 10, 2591-2599.
Crossref Google Scholar
[14]
J. Gilleron,; W. Querbes,; A. Zeigerer,; A. Borodovsky,; G. Marsico,; U. Schubert,; K. Manygoats,; S. Seifert,; C. Andree,; M. Stöter, et al. Image-based analysis of lipid nanoparticle-mediated siRNA delivery, intracellular trafficking and endosomal escape. Nat. Biotechnol. 2013, 31, 638-646.
Crossref Google Scholar
[15]
P. F. Wang,; M. A. Rahman,; Z. X. Zhao,; K. Weiss,; C. Zhang,; Z. J. Chen,; S. J. Hurwitz,; Z. G. Chen,; D. M. Shin,; Y. G. Ke, Visualization of the cellular uptake and trafficking of DNA origami nanostructures in cancer cells. J. Am. Chem. Soc. 2018, 140, 2478-2484.
Crossref Google Scholar
[16]
G. B. Qi,; Y. J. Gao,; L. Wang,; H. Wang, Self-assembled peptide-based nanomaterials for biomedical imaging and therapy. Adv. Mater. 2018, 30, 1703444.
Crossref Google Scholar
[17]
K. Sato,; M. P. Hendricks,; L. C. Palmer,; S. I. Stupp, Peptide supramolecular materials for therapeutics. Chem. Soc. Rev. 2018, 47, 7539-7551.
Crossref Google Scholar
[18]
M. Li,; M. Ehlers,; S. Schlesiger,; E. Zellermann,; S. K. Knauer,; C. Schmuck, Incorporation of a non-natural arginine analogue into a cyclic peptide leads to formation of positively charged nanofibers capable of gene transfection. Angew. Chem., Int. Ed. 2016, 55, 598-601.
Crossref Google Scholar
[19]
W. S. Zhang,; D. M. Lin,; H. X. Wang,; J. F. Li,; G. U. Nienhaus,; Z. Q. Su,; G. Wei,; L. Shang, Supramolecular self-assembly bioinspired synthesis of luminescent gold nanocluster-embedded peptide nanofibers for temperature sensing and cellular imaging. Bioconjugate Chem. 2017, 28, 2224-2229.
Crossref Google Scholar
[20]
Z. Fan,; L. M. Sun,; Y. J. Huang,; Y. Z. Wang,; M. J. Zhang, Bioinspired fluorescent dipeptide nanoparticles for targeted cancer cell imaging and real-time monitoring of drug release. Nat. Nanotechnol. 2016, 11, 388-394.
Crossref Google Scholar
[21]
Y. Wang,; Y. X. Lin,; Z. Y. Qiao,; H. W. An,; S. L. Qiao,; L. Wang,; R. P. Y. J. Rajapaksha,; H. Wang, Self-assembled autophagy-inducing polymeric nanoparticles for breast cancer interference in-vivo. Adv. Mater. 2015, 27, 2627-2634.
Crossref Google Scholar
[22]
Z. Fan,; Y. Chang,; C. C. Cui,; L. M. Sun,; D. H. Wang,; Z. Pan,; M. J. Zhang, Near infrared fluorescent peptide nanoparticles for enhancing esophageal cancer therapeutic efficacy. Nat. Commun. 2018, 9, 2605.
Crossref Google Scholar
[23]
R. Pugliese,; A. Marchini,; G. A. A. Saracino,; R. N. Zuckermann,; F. Gelain, Cross-linked self-assembling peptide scaffolds. Nano Res. 2018, 11, 586-602.
Crossref Google Scholar
[24]
J. B. Liu,; M. X. Yu,; C. Zhou,; S. Y. Yang,; X. H. Ning,; J. Zheng, Passive tumor targeting of renal-clearable luminescent gold nanoparticles: Long tumor retention and fast normal tissue clearance. J. Am. Chem. Soc. 2013, 135, 4978-4981.
Crossref Google Scholar
[25]
J. B. Liu,; P. N. Duchesne,; M. X. Yu,; X. Y. Jiang,; X. H. Ning,; R. D. Vinluan III,; P. Zhang,; J. Zheng, Luminescent gold nanoparticles with size-independent emission. Angew. Chem., Int. Ed. 2016, 55, 8894-8898.
Crossref Google Scholar
[26]
M. R. Ghadiri,; J. R. Granja,; R. A. Milligan,; D. E. McRee,; N. Khazanovich, Self-assembling organic nanotubes based on a cyclic peptide architecture. Nature 1993, 366, 324-327.
Crossref Google Scholar
[27]
A. Lamas,; A. Guerra,; M. Amorín,; J. R. Granja, New self-assembling peptide nanotubes of large diameter using δ-amino acids. Chem. Sci. 2018, 9, 8228-8233.
Crossref Google Scholar
[28]
T. Y. Zhou,; J. Y. Zhu,; L. S. Gong,; L. T. Nong,; J. B. Liu, Amphiphilic block copolymer-guided in situ fabrication of stable and highly controlled luminescent copper nanoassemblies. J. Am. Chem. Soc. 2019, 141, 2852-2856.
Crossref Google Scholar
[29]
D. S. Ling,; M. J. Hackett,; T. Hyeon, Surface ligands in synthesis, modification, assembly and biomedical applications of nanoparticles. Nano Today 2014, 9, 457-477.
Crossref Google Scholar
[30]
B. D. Chithrani,; W. C. W. Chan, Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano Lett. 2007, 7, 1542-1550.
Crossref Google Scholar
[31]
J. X. Lu,; J. Wang,; D. S. Ling, Surface engineering of nanoparticles for targeted delivery to hepatocellular carcinoma. Small 2018, 14, 1702037.
Google Scholar
[32]
X. A. Wu,; C. H. J. Choi,; C. Zhang,; L. L. Hao,; C. A. Mirkin, Intracellular fate of spherical nucleic acid nanoparticle conjugates. J. Am. Chem. Soc. 2014, 136, 7726-7733.
Google Scholar
[33]
G. Vindigni,; S. Raniolo,; A. Ottaviani,; M. Falconi,; O. Franch,; B. R. Knudsen,; A. Desideri,; S. Biocca, Receptor-mediated entry of pristine octahedral DNA nanocages in mammalian cells. ACS Nano 2016, 10, 5971-5979.
Google Scholar
[34]
V. Zinchuk,; O. Zinchuk,; T. Okada, Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: Pushing pixels to explore biological phenomena. Acta Histochem. Cytochem. 2007, 40, 101-111.
Google Scholar
[35]
J. Y. Zhu,; K. He,; Z. Y. Dai,; L. S. Gong,; T. Y. Zhou,; H. R. Liang,; J. B. Liu, Self-assembly of luminescent gold nanoparticles with sensitive pH-stimulated structure transformation and emission response toward lysosome escape and intracellular imaging. Anal. Chem. 2019, 91, 8237-8243.
Google Scholar
[36]
T. G. Iversen,; T. Skotland,; K. Sandvig, Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies. Nano Today 2011, 6, 176-185.
Google Scholar
[37]
J. Huang,; C. Zong,; H. Shen,; M. Liu,; B. Chen,; B. Ren,; Z. J. Zhang, Mechanism of cellular uptake of graphene oxide studied by surface-enhanced Raman spectroscopy. Small 2012, 8, 2577-2584.
Google Scholar
[38]
M. Akishiba,; T. Takeuchi,; Y. Kawaguchi,; K. Sakamoto,; H. H. Yu,; I. Nakase,; T. Takatani-Nakase,; F. Madani,; A. Gräslund,; S. Futaki, Cytosolic antibody delivery by lipid-sensitive endosomolytic peptide. Nat. Chem. 2017, 9, 751-761.
Google Scholar
[39]
S. E. A. Gratton,; P. A. Ropp,; P. D. Pohlhaus,; J. C. Luft,; V. J. Madden,; M. E. Napier,; J. M. DeSimone, The effect of particle design on cellular internalization pathways. Proc. Natl. Acad. Sci. USA 2008, 105, 11613-11618.
Google Scholar
[40]
P. Jana,; K. Samanta,; S. Bäcker,; E. Zellermann,; S. Knauer,; C. Schmuck, Efficient gene transfection through inhibition of β-sheet (amyloid fiber) formation of a short amphiphilic peptide by gold nanoparticles. Angew. Chem., Int. Ed. 2017, 56, 8083-8088.
Google Scholar
[41]
Y. H. Liu,; X. N. Zhang,; C. Han,; G. H. Wan,; X. X. Huang,; C. Ivan,; D. H. Jiang,; C. Rodriguez-Aguayo,; G. Lopez-Berestein,; P. H. Rao, et al. TP53 loss creates therapeutic vulnerability in colorectal cancer. Nature 2015, 520, 697-701.
Google Scholar
[42]
V. J. N. Bykov,; S. E. Eriksson,; J. Bianchi,; K. G. Wiman, Targeting mutant p53 for efficient cancer therapy. Nat. Rev. Cancer 2017, 18, 89-102.
Google Scholar
[43]
U. Capasso Palmiero,; J. C. Kaczmarek,; O. S. Fenton,; D. G. Anderson, Poly(β-amino ester)-co-poly(caprolactone) terpolymers as nonviral vectors for mRNA delivery in vitro and in vivo. Adv. Healthcare Mater. 2018, 7, 1800249.
Google Scholar
[44]
M. Takagi,; M. J. Absalon,; K. G. McLure,; M. B. Kastan, Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell 2005, 123, 49-63.
Google Scholar
[45]
M. H. G. Kubbutat,; S. N. Jones,; K. H. Vousden, Regulation of p53 stability by Mdm2. Nature 1997, 387, 299-303.
Google Scholar
[46]
Q. Chen,; C. Wang,; X. D. Zhang,; G. J. Chen,; Q. Y. Hu,; H. J. Li,; J. Q. Wang,; D. Wen,; Y. Q. Zhang,; Y. F. Lu, et al. In situ sprayed bioresponsive immunotherapeutic gel for post-surgical cancer treatment. Nat. Nanotechnol. 2019, 14, 89-97.
Google Scholar
Nano Research
Volume 14 Issue 4,
April 2021
Pages 1087-1094
DOI: 10.1007/s12274-020-3153-6
Cite this article:
He K, Zhu J, Gong L, et al. In situ self-assembly of near-infrared-emitting gold nanoparticles into body-clearable 1D nanostructures with rapid lysosome escape and fast cellular excretion. Nano Research, 2021, 14(4): 1087-1094. https://doi.org/10.1007/s12274-020-3153-6
Topics:
Cell cultureDNA GenesGold nanoparticlesMolecular biologyMolecular imagingNanoparticlesProteinsSelf assembly

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Received: 27 July 2020
Revised: 29 September 2020
Accepted: 01 October 2020
Published: 06 November 2020
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature
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