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Research Article

Multi-shell nanocomposites based multienzyme mimetics for efficient intracellular antioxidation

Yongjian Ai1, Jinzhi You1, Jianyi Gao2, Jiaping Wang2, Hong-bin Sun3, Mingyu Ding1, Qionglin Liang1( )
MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Beijing Key Lab of Microanalytical Methods & Instrumentation, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
China Astronaut Research and Training Center, Beijing 100094, China
Department of Chemistry, Northeastern University, Shenyang 110819, China
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Abstract

Oxidative stress is associated with many acute and chronic inflammatory diseases. Development of nanomaterial-based enzyme mimetics for reactive oxygen species (ROS) scavenging is challenging, but holds great promise for the treatment of inflammatory diseases. Herein, we report the highly ordered manganese dioxide encapsulated selenium-melanin (Se@Me@MnO2) nanozyme with high efficiency for intracellular antioxidation and anti-inflammation. The Se@Me@MnO2 nanozyme is sequentially fabricated through the radical polymerization and the in-situ oxidation-reduction. In vitro experimental results demonstrated that the Se@Me@MnO2 nanozyme exhibits multiple enzyme activities to scavenge ROS, including catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD). Mechanism researches illustrated that the Se core possesses GPx-like catalytic activity, the Me and the MnO2 possess both the SOD-like and the CAT-like activities. What’s more, due to the stable unpaired electrons existing in the nanozyme, the Se, Me and MnO2 provide synergistic and fast electron transfer effect to achieve the quickly scavenging of hydrogen peroxide, hydroxyl radical, and superoxide anion. Further in vivo experimental results showed that this biocompatible nanozyme exhibits cytoprotective effects by resisting ROS-mediated damage, thereby alleviating the inflammation. This multienzyme mimetics is believed to be an excellent ROS scavenger and have a good potential in clinical therapy for ROS-related diseases.

Keywords

oxidative stressreactive oxygen speciesmultienzyme mimeticsnanozymesintracellular antioxidationanti-inflammation

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Nano Research
Volume 14 Issue 8,
August 2021
Pages 2644-2653
DOI: 10.1007/s12274-020-3267-x
Cite this article:
Ai Y, You J, Gao J, et al. Multi-shell nanocomposites based multienzyme mimetics for efficient intracellular antioxidation. Nano Research, 2021, 14(8): 2644-2653. https://doi.org/10.1007/s12274-020-3267-x
Topics:
BiocompatibilityCatalyst activityEnzymesFree radical reactionsManganese oxidePathologySelenium compounds

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Received: 17 August 2020
Revised: 22 November 2020
Accepted: 24 November 2020
Published: 25 January 2021
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
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