Home Nano Research Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Graphical Abstract
Abstract
Keywords
Electronic Supplementary Material
References
Show full outline
Hide outline
Research Article

Black phosphorus quantum dots as multifunctional nanozymes for tumor photothermal/catalytic synergistic therapy

Hui Ding1,3, Daji Wang1,3, Haibing Huang1, Xiaozhu Chen1, Jie Wang1, Jinjie Sun1, Jianlin Zhang2, Lu Lu1, Beiping Miao1, Yanjuan Cai1, Kelong Fan3, Yongtian Lu1, Hongsong Dong1, Xiyun Yan3(), Guohui Nie1(), Minmin Liang2()
Shenzhen Key Laboratory of Nanozymes and Translational Cancer Research, Department of OtolaryngologyInstitute of Translational Medicine, Shenzhen Second People's Hospital/the First Affiliated Hospital of Shenzhen University Health Science CenterShenzhen518035China
Experimental Center of Advanced MaterialsSchool of Materials Science and Engineering, Beijing Institute of TechnologyBeijing100081China
Key Laboratory of Protein and Peptide PharmaceuticalsInstitute of Biophysics, Chinese Academy of SciencesBeijing100101China
Show Author Information
An erratum to this article is available online at:
https://doi.org/10.1007/s12274-022-5087-7

Graphical Abstract

View original image Download original image

Abstract

Nanozymes are nanomaterials with enzyme-like properties that have attracted significant interest owing to their high stability, easy preparation, and tunable catalytic properties, especially in the field of cancer therapy. However, the unfavorable catalytic effects of nanozymes in the acidic tumor microenvironment have limited their applications. Herein, we developed a biomimetic erythrocyte membrane-camouflaged ultrasmall black phosphorus quantum dots (BPQDs) nanozymes that simultaneously exhibited an exceptional near-infrared (NIR) photothermal property and dramatically photothermal-enhanced glucose oxidase (GOx)-like activity in the acidic tumor microenvironment. We demonstrated the engineered BPQDs gave a photothermal conversion efficiency of 28.9% that could rapidly heat the tumor up to 50 ℃ while effectively localized into tumors via homing peptide iRGD leading after intravenously injection. Meanwhile, the significantly enhanced GOx-like activity of BPQDs under NIR irradiation was capable of catalytical generating massive toxic reactive oxygen species via using cellular glucose. By combining the intrinsic photothermal property and the unique photothermal-enhanced GOx-like catalytic activity, the developed BPQDs were demonstrated to be an effective therapeutic strategy for inhibiting tumor growth in vivo. We believe that this work will provide a novel perspective for the development of nanozymes in tumor catalytic therapy.

Keywords

nanozymesblack phosphorus quantum dotsglucose oxidase-like activityphotothermal/catalytic synergistic therapy

Electronic Supplementary Material

Download File(s)
12274_2021_3701_MOESM1_ESM.pdf (3.8 MB)

References

1

Wu, J. J. X.; Wang, X. Y.; Wang, Q.; Lou, Z. P.; Li, S. R.; Zhu, Y. P.; Qin, L.; Wei, H. Nanomaterials with enzyme-like characteristics (nanozymes): Next-generation artificial enzymes (Ⅱ). Chem. Soc. Rev. 2019, 48, 1004-1076.

Crossref Google Scholar
2

Huang, Y. Y.; Ren, J. S.; Qu, X. G. Nanozymes: Classification, catalytic mechanisms, activity regulation, and applications. Chem. Rev. 2019, 119, 4357-4412.

Crossref Google Scholar
3

Gao, L. Z.; Zhuang, J.; Nie, L.; Zhang, J. B.; Zhang, Y.; Gu, N.; Wang, T. H.; Feng, J.; Yang, D. L.; Perrett, S. et al. Intrinsic peroxidase- like activity of ferromagnetic nanoparticles. Nat. Nanotechnol. 2007, 2, 577-583.

Crossref Google Scholar
4

Liang, M. M.; Yan, X. Y. Nanozymes: From new concepts, mechanisms, and standards to applications. Acc. Chem. Res. 2019, 52, 2190-2200.

Crossref Google Scholar
5

Cormode, D. P.; Gao, L. Z.; Koo, H. Emerging biomedical applications of enzyme-like catalytic nanomaterials. Trends Biotechnol. 2018, 36, 15-29.

Crossref Google Scholar
6

Song, W.; Zhao, B.; Wang, C.; Ozaki, Y.; Lu, X. F. Functional nanomaterials with unique enzyme-like characteristics for sensing applications. J. Mater. Chem. B 2019, 7, 850-875.

Crossref Google Scholar
7

Lin, H.; Chen, Y.; Shi, J. L. Nanoparticle-triggered in situ catalytic chemical reactions for tumour-specific therapy. Chem. Soc. Rev. 2018, 47, 1938-1958.

Crossref Google Scholar
8

Munir, S.; Shah, A. A.; Rahman, H.; Bilal, M.; Rajoka, M. S. R.; Khan, A. A.; Khurshid, M. Nanozymes for medical biotechnology and its potential applications in biosensing and nanotherapeutics. Biotechnol. Lett. 2020, 42, 357-373.

Crossref Google Scholar
9

Fan, K. L.; Xi, J. Q.; Fan, L.; Wang, P. X.; Zhu, C. H.; Tang, Y.; Xu, X. D.; Liang, M. M.; Jiang, B.; Yan, X. Y. et al. In vivo guiding nitrogen-doped carbon nanozyme for tumor catalytic therapy. Nat. Commun. 2018, 9, 1440-1451.

Crossref Google Scholar
10

Zhu, P.; Chen, Y.; Shi, J. L. Nanoenzyme-augmented cancer sonodynamic therapy by catalytic tumor oxygenation. ACS Nano 2018, 12, 3780-3795.

Crossref Google Scholar
11

Korschelt, K.; Tahir, M. N.; Tremel, W. A step into the future: Applications of nanoparticle enzyme mimics. Chem. Eur. J. 2018, 24, 9703-9713.

Crossref Google Scholar
12

Huo, M. F.; Wang, L. Y.; Wang, Y. W.; Chen, Y.; Shi, J. L. Nanocatalytic tumor therapy by single-atom catalysts. ACS Nano 2019, 13, 2643-2653.

Crossref Google Scholar
13

Wang, Z. Z.; Zhang, Y.; Ju, E. G.; Liu, Z.; Cao, F. F.; Chen, Z. W.; Ren, J. S.; Qu, X. G. Biomimetic nanoflowers by self-assembly of nanozymes to induce intracellular oxidative damage against hypoxic tumors. Nat. Commun. 2018, 9, 3334.

Crossref Google Scholar
14

Fan, L.; Xu, X. D.; Zhu, C. H.; Han, J.; Gao, L. Z.; Xi, J. Q.; Guo, R. Tumor catalytic-photothermal therapy with yolk-shell gold@carbon nanozymes. ACS Appl. Mater. Interfaces 2018, 10, 4502-4511.

Crossref Google Scholar
15

Robinson, J. T.; Tabakman, S. M.; Liang, Y. Y.; Wang, H. L.; Casalongue, H. S.; Vinh, D.; Dai, H. J. Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. J. Am. Chem. Soc. 2011, 133, 6825-6831.

Crossref Google Scholar
16

Ding, P. H.; Di, J.; Chen, X. L.; Ji, M. X.; Gu, K. Z.; Yin, S.; Liu, G. P.; Zhang, F.; Xia, J. X.; Li, H. M. S, N codoped graphene quantum dots embedded in (BiO)2CO3: Incorporating enzymatic-like catalysis in photocatalysis. ACS Sustainable Chem. Eng. 2018, 6, 10229-10240.

Crossref Google Scholar
17

Zhang, X.; Xie, H. M.; Liu, Z. D.; Tan, C. L.; Luo, Z. M.; Li, H.; Lin, J. D.; Sun, L. Q.; Chen, W.; Xu, Z. C. et al. Black phosphorus quantum dots. Angew. Chem., Int. Ed. 2015, 54, 3653-3657.

Crossref Google Scholar
18

Mu, X. Y.; Wang, J. Y.; Bai, X. T.; Xu, F. J.; Liu, H. X.; Yang, J.; Jing, Y. Q.; Liu, L. F.; Xue, X. H.; Dai, H. T. et al. Black phosphorus quantum dot induced oxidative stress and toxicity in living cells and mice. ACS Appl. Mater. Interfaces 2017, 9, 20399-20409.

Crossref Google Scholar
19

Gui, R. J.; Jin, H.; Wang, Z. H.; Li, J. H. Black phosphorus quantum dots: Synthesis, properties, functionalized modification and applications. Chem. Soc. Rev. 2018, 47, 6795-6823.

Crossref Google Scholar
20

Zeng, X. W.; Luo, M. M.; Liu, G.; Wang, X. S.; Tao, W.; Lin, Y. X.; Ji, X. Y.; Nie, L.; Mei, L. Polydopamine-modified black phosphorous nanocapsule with enhanced stability and photothermal performance for tumor multimodal treatments. Adv. Sci. 2018, 5, 1800510.

Crossref Google Scholar
21

Sun, Z. B.; Xie, H. H.; Tang, S. Y.; Yu, X. F.; Guo, Z. N.; Shao, J. D.; Zhang, H.; Huang, H.; Wang, H. Y.; Chu, P. K. Ultrasmall black phosphorus quantum dots: Synthesis and use as photothermal agents. Angew. Chem., Int. Ed. 2015, 54, 11526-11530.

Crossref Google Scholar
22

Hu, Y. H.; Cheng, H. J.; Zhao, X. Z.; Wu, J. J. X.; Muhammad, F.; Lin, S. C.; He, J.; Zhou, L. Q.; Zhang, C. P.; Deng, Y. et al. Surface-enhanced Raman scattering active gold nanoparticles with enzyme-mimicking activities for measuring glucose and lactate in living tissues. ACS Nano 2017, 11, 5558-5566.

Crossref Google Scholar
23

Huo, M. F.; Wang, L. Y.; Chen, Y.; Shi, J. L. Tumor-selective catalytic nanomedicine by nanocatalyst delivery. Nat. Commun. 2017, 8, 357.

Crossref Google Scholar
24

Ni, D. Z.; Ding, H.; Liu, S.; Yue, H.; Bao, Y. L.; Wang, Z. H.; Su, Z. G.; Wei, W.; Ma, G. H. Superior intratumoral penetration of paclitaxel nanodots strengthens tumor restriction and metastasis prevention. Small 2015, 11, 2518-2526.

Crossref Google Scholar
25

Roper, D. K.; Ahn, W.; Hoepfner, M. Microscale heat transfer transduced by surface plasmon resonant gold nanoparticles. J. Phys. Chem. C 2007, 111, 3636-3641.

Crossref Google Scholar
26

Hu, C. M. J.; Zhang, L.; Aryal, S.; Cheung, C.; Fang, R. H.; Zhang, L. F. Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform. Proc. Natl. Acad. Sci. USA 2011, 108, 10980-10985.

Crossref Google Scholar
27

Ding, H.; Cai, Y. J.; Gao, L. Z.; Liang, M. M.; Miao, B. P.; Wu, H. W.; Liu, Y.; Xie, N.; Tang, A. F.; Fan, K. L. et al. Exosome-like nanozyme vesicles for H2O2-responsive catalytic photoacoustic imaging of xenograft nasopharyngeal carcinoma. Nano Lett. 2019, 19, 203-209.

Crossref Google Scholar
28

Waite, C. L.; Roth, C. M. Nanoscale drug delivery systems for enhanced drug penetration into solid tumors: Current progress and opportunities. Crit. Rev. Biomed. Eng. 2012, 40, 21-41.

Crossref Google Scholar
29

Sugahara, K. N.; Teesalu, T.; Karmali, P. P.; Kotamraju, V. R.; Agemy, L.; Greenwald, D. R.; Ruoslahti, E. Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs. Science 2010, 328, 1031-1035.

Crossref Google Scholar
30

Peng, Z. H.; Kopeček, J. Enhancing accumulation and penetration of HPMA copolymer-doxorubicin conjugates in 2D and 3D prostate cancer cells via iRGD conjugation with an MMP-2 cleavable spacer. J. Am. Chem. Soc. 2015, 137, 6726-6729.

Crossref Google Scholar
31

Ni, D. Z.; Ding, H.; Liu, S.; Yue, H.; Bao, Y. L.; Wang, Z. H.; Su, Z. G.; Wei, W.; Ma, G. H. Superior intratumoral penetration of paclitaxel nanodots strengthens tumor restriction and metastasis prevention. Small 2015, 11, 2518-2526.

Crossref Google Scholar
32

Ding, H.; Zhang, F.; Zhao, C. C.; Lv, Y. L.; Ma, G. H.; Wei, W.; Tian, Z. Y. Beyond a carrier: Graphene quantum dots as a probe for programmatically monitoring anti-cancer drug delivery, release, and response. ACS Appl. Mater. Interfaces 2017, 9, 27396-27401.

Crossref Google Scholar
33

Ding, H.; Lv, Y. L.; Ni, D. Z.; Wang, J.; Tian, Z. Y.; Wei, W.; Ma, G. H. Erythrocyte membrane-coated NIR-triggered biomimetic nanovectors with programmed delivery for photodynamic therapy of cancer. Nanoscale 2015, 7, 9806-9815.

Crossref Google Scholar
34

Yang, Y.; Zhu, W. J.; Dong, Z. L.; Chao, Y.; Xu, L.; Chen, M. W.; Liu, Z. 1D coordination polymer nanofibers for low-temperature photothermal therapy. Adv. Mater. 2017, 29, 1703588.

Crossref Google Scholar
35

Yang, Y. C.; He, P.; Wang, Y. X.; Bai, H. T.; Wang, S.; Xu, J. F.; Zhang, X. Supramolecular radical anions triggered by bacteria in situ for selective photothermal therapy. Angew. Chem., Int. Ed. 2017, 56, 16239-16242.

Crossref Google Scholar
36

Yang, K.; Zhang, S.; Zhang, G. X.; Sun, X. M.; Lee, S. T.; Liu, Z. Graphene in mice: Ultrahigh in vivo tumor uptake and efficient photothermal therapy. Nano Lett. 2010, 10, 3318-3323.

Crossref Google Scholar
Nano Research
Volume 15 Issue 2,
February 2022
Pages 1554-1563
DOI: 10.1007/s12274-021-3701-8
Cite this article:
Ding H, Wang D, Huang H, et al. Black phosphorus quantum dots as multifunctional nanozymes for tumor photothermal/catalytic synergistic therapy. Nano Research, 2022, 15(2): 1554-1563. https://doi.org/10.1007/s12274-021-3701-8
Topics:
Biomaterials
Metrics & Citations  
Article History
Copyright
Return