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Research Article

Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model

Qun Huang1,§, Yuting Cai2,4,§, Xinrui Yang1,3,§, Weimin Li1,3,§, Hongji Pu1, Zhenjing Liu4, Hongwei Liu4, Mohsen Tamtaji4, Feng Xu5, Liyuan Sheng6, Tae-Hyung Kim7, Shiqing Zhao8, Dazhi Sun2( ), Jinbao Qin1,3( ), Zhengtang Luo4( ), Xinwu Lu1,3( )
Department of Vascular Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
Guangdong Provincial Key Laboratory of Functional Oxide Materials and Devices, Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Vascular Center of Shanghai Jiao Tong University, Shanghai 200011, China
Department of Chemical and Biological Engineering, and William Mong Institute of Nano Science and Technology, the Hong Kong University of Science and Technology, Hong Kong 999077, China
Bioinspired Engineering and Biomechanics Center (BEBC), the Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
Shenzhen Institute, Peking University, Shenzhen 518057, China
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China

§ Qun Huang, Yuting Cai, Xinrui Yang, and Weimin Li contributed equally to this work.

Show Author Information

Graphical Abstract

Adipose-derived stem cells (ADSCs)-loaded graphene foam/hydrogel scaffold promotes the recovery of diabetic peripheral nerve injury.

Abstract

The functional recovery of peripheral nerve injury (PNI) is unsatisfactory, whereas diabetes mellitus (DM) and its related complications further attenuate the restoration of diabetic PNI (DPNI). Adipose-derived stem cells (ADSCs) are promising candidates for treatment of DPNI due to their abundant source, excellent differentiation and paracrine ability. Our results showed that ADSCs remarkably enhanced the proliferation and migration of Schwann cells and endothelial cells, and tube formation. Mechanistically, ADSCs could regulate Nrf2/HO-1, NF-κB and PI3K/AKT/mTOR signaling pathways, showing multiple functions in reducing oxidative stress and inflammation, and regulating cell metabolism, growth, survival, proliferation, angiogenesis, differentiation of Schwann cell and myelin formation. In current study, novel graphene foam (GF)/hydrogel-based scaffold was developed to deliver ADSCs for treatment of DPNI. GF/hydrogel scaffold exhibited excellent mechanical strength, suitable porous network, superior electrical conductivity, and good biocompatibility. In vitro results revealed that GF/hydrogel scaffold could obviously accelerate proliferation of Schwann cells. Moreover, in vivo experiments demonstrated that ADSCs-loaded GF/hydrogel scaffold significantly promoted the recovery of DPNI and inhibited the atrophy of targeted muscles, thus providing a novel and attractive therapeutic approach for DPNI patients.

Keywords

peripheral nerve injury (PNI)diabetes mellitus (DM)adipose-derived stem cells (ADSCs)graphenehydrogel scaffold

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Nano Research
Volume 15 Issue 4,
April 2022
Pages 3434-3445
DOI: 10.1007/s12274-021-3961-3
Cite this article:
Huang Q, Cai Y, Yang X, et al. Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model. Nano Research, 2022, 15(4): 3434-3445. https://doi.org/10.1007/s12274-021-3961-3
Topics:
Tissue engineering

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Received: 25 July 2021
Revised: 26 October 2021
Accepted: 29 October 2021
Published: 15 December 2021
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
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