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Research Article

A photonanozyme with light-empowered specific peroxidase-mimicking activity

Sili Lin§, Wenlong Tan§, Pengfei Han, Xu Li, Jinzhao Li, Zhou Nie, Kun Li( )
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China

§ Sili Lin and Wenlong Tan contributed equally to this work.

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Graphical Abstract

Light as a direct energy source driving photonanozymatic reactions with H2O2 enables TiO2 photonanozymes to have the peroxidase-like specificity exclusively under illumination. Photogenerated hot charge carriers and the accompanied reactive oxygen species are responsible for light-empowered photonanozymatic activity, while co-substrates are conducive to the formation of the photo-reactive peroxo-oxygen bridge bond between TiO2 and H2O2 that specifically allows the peroxidase-like activity.

Abstract

Although nanozymes have been widely developed, directly utilizing light to drive catalytic reactions like natural photoenzymes still remains challenging. Herein, we propose that photonanozymes (PNZs), as a novel kind of nanozyme, exclusively possess enzyme-mimicking activity under illumination. Only in the presence of visible light, the as-synthesized TiO2 proposed in this contribution shows excellent specificity of peroxidase-like without any oxidase- or catalase-like activity. The driving force of the light-empowered peroxidase-like photonanozymatic activity is explicated in terms of the photogenerated hot charge carriers in TiO2 PNZs and the accompanied reactive oxygen species. The co-substrates for photonanozymatic reaction over TiO2 PNZs facilitate the formation of the precarious and reactive peroxo-oxygen bridge between TiO2 and H2O2, enabling the catalytic specificity. With the TiO2 PNZ-based biosensing platform for visual glucose detection exemplifying the concept of the application of PNZs, this work may evoke more inspirations to explore strategies for enlarging the scope of photoenzyme mimics.

Keywords

photonanozymetitanium dioxideperoxidase mimicsspecificitybiosensors

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Nano Research
Volume 15 Issue 10,
October 2022
Pages 9073-9081
DOI: 10.1007/s12274-022-4538-5
Cite this article:
Lin S, Tan W, Han P, et al. A photonanozyme with light-empowered specific peroxidase-mimicking activity. Nano Research, 2022, 15(10): 9073-9081. https://doi.org/10.1007/s12274-022-4538-5
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Received: 11 April 2022
Revised: 13 May 2022
Accepted: 15 May 2022
Published: 25 June 2022
© Tsinghua University Press 2022
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