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Research Article

Near-field electrospun PCL fibers/GelMA hydrogel composite dressing with controlled deferoxamine-release ability and retiform surface for diabetic wound healing

Huiling Zhong1,2,§, Jun Huang3,§, Moucheng Luo2, Yifei Fang1,2, Xinchen Zeng4, Jun Wu1,2( ), Jianhang Du1,5( )
Medical Research Center, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, China
School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, China
Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, China
Department of Liver Surgery, The Third People’s Hospital of Shenzhen, Shenzhen 518112, China
NHC Key Laboratory of Assisted Circulation, Sun Yat-sen University, Guangzhou 510080, China

§ Huiling Zhong and Jun Huang contributed equally to this work.

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Graphical Abstract

PCL-GelMA-DFO hydrogel helps promoting angiogenesis and wound healing.

Abstract

Wound ulceration caused by diabetes is a typical chronic wound wherein healing the local tissue is difficult due to lack of blood vessels and tissue necrosis caused by the long-term accumulation of free radicals. Near-field electrospinning (NFES) is an innovative technology used to produce micro-nano-scaled, controllable sequencing fibers. In this study, we constructed a novel wound dressing based on the NFES polycaprolactone (PCL) fiber network and modified gelatin with methacrylic anhydride (GelMA) hydrogel to promote angiogenesis and the re-epithelialization of diabetic wounds. An angiogenic and antioxidant drug named deferoxamine (DFO) was encapsulated in a GelMA hydrogel to achieve a slow-release effect that is more suitable for chronic wounds. The cell adhesion experiment showed that the cells could attach to the fibers in the dressing group having a network of PCL fibers on the surface and grow along the direction of the fibers, which in turn, effectively regulates cell behavior from the physical structure. Additionally, the large pore size (~ 500 μm) of the network allowed the cells to penetrate the pores and enter the surface of the hydrogel without being blocked out. Besides, the composite dressing had a notable effect on angiogenesis. Furthermore, antioxidation experiments confirmed that the DFO-loaded hydrogel exhibited antioxidant activity. Experimental animal models of diabetes showed that rats treated with the PCL-GelMA-DFO (PGD) hydrogel had faster ability of hemostasis, scab formation, and wound healing. In conclusion, the PGD hydrogel effectively promoted the repair of chronic wounds.

Keywords

diabetic wound healingnear field electrospinninghydrogellarge pore

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Nano Research
Volume 16 Issue 1,
January 2023
Pages 599-612
DOI: 10.1007/s12274-022-4813-5
Cite this article:
Zhong H, Huang J, Luo M, et al. Near-field electrospun PCL fibers/GelMA hydrogel composite dressing with controlled deferoxamine-release ability and retiform surface for diabetic wound healing. Nano Research, 2023, 16(1): 599-612. https://doi.org/10.1007/s12274-022-4813-5
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Received: 14 June 2022
Revised: 19 July 2022
Accepted: 25 July 2022
Published: 03 September 2022
© Tsinghua University Press 2022
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