AI Chat Paper
Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
Search articles, authors, keywords, DOl and etc.
{{expandStatus?'Exit ':''}}Advanced Search
Coronavirus disease 2019 (COVID-19) highlights the importance of rapid and reliable diagnostic assays for the management of virus transmission. Here, we developed a one-pot hydrothermal method to prepare Si-FITC nanoparticles (NPs) for the fluorescent immunoassay of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (N protein). The synthesis of Si-FITC NPs did not need post-modification, which addressed the issue of quantum yield reduction during the coupling reaction. Si-FITC NPs showed two distinct peaks, Si fluorescence at λem = 385 nm and FITC fluorescence at λem = 490 nm. In the presence of KMnO4, Si fluorescence was decreased and FITC fluorescence was enhanced. Briefly, in the presence of N protein, catalase (CAT)-linked secondary antibody/reporter antibody/N protein/capture antibody immunocomplexes were formed on microplates. Subsequently, hydrogen peroxide (H2O2) and Si-FITC NPs/KMnO4 were injected into the microplate together. The decomposition of H2O2 by CAT resulted in remaining of KMnO4, which changed the fluorescence intensity ratio of Si-FITC NPs. The fluorescence intensity ratio correlated significantly with the N protein concentration ranging from 0.02 to 50.00 ng/mL, and the detection limit was 0.003 ng/mL, which was more sensitive than the commercial ELISA kit with a detection limit of 0.057 ng/mL. The N protein concentration can be accurately determined in human serum. Furthermore, the COVID-19 and non-COVID-19 patients were distinguishable by this method. Therefore, the ratiometric fluorescent immunoassay can be used for SARS-CoV-2 infection diagnosis with a high sensitivity and selectivity.
Zhu, N.; Zhang, D.; Wang, W.; Li, X.; Yang, B.; Song, J.; Zhao, X.; Huang, B.; Shi, W.; Lu, R. et al. A novel coronavirus from patients with pneumonia in China, 2019. New. Engl. J. Med. 2020, 382, 727–733.
Zhou, P.; Yang, X. L.; Wang, X. G.; Hu, B.; Zhang, L.; Zhang, W.; Si, H. R.; Zhu, Y.; Li, B.; Huang, C. L. et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 2020, 579, 270–273.
Rosenthal, P. J. The importance of diagnostic testing during a viral pandemic: early lessons from novel coronavirus disease (COVID-19). Am. J. Trop. Med. Hyg. 2020, 102, 915–916.
Yang, Y.; Yang, M.; Yuan, J.; Wang, F.; Wang, Z.; Li, J.; Zhang, M.; Xing, L.; Wei, J.; Peng, L. et al. Laboratory diagnosis and monitoring the viral shedding of SARS-CoV-2 infection. Innovation (N Y) 2020, 1, 100061.
Wiersinga, W. J.; Rhodes, A.; Cheng, A. C.; Peacock, S. J.; Prescott, H. C. Pathophysiology, transmission, diagnosis, and treatment of coronavirus disease 2019 (COVID-19) a review. JAMA. 2020, 324, 782–793.
Vogels, C. B. F.; Brito, A. F.; Wyllie, A. L.; Fauver, J. R.; Ott, I. M.; Kalinich, C. C.; Petrone, M. E.; Casanovas-Massana, A.; Muenker, M. C.; Moore, A. J. et al. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets. Nat. Microbiol. 2020, 5, 1299–1305+.
Wang, Y.; Kang, H.; Liu, X.; Tong, Z. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J. Med. Virol. 2020, 92, 538–539.
Miller, S.; Chiu, C.; Rodino, K. G.; Miller, M. B. Point-counterpoint: should we be performing metagenomic next-generation sequencing for infectious disease diagnosis in the clinical laboratory? J. Clin. Microbiol. 2020, 58, e01739–19.
Augustine, R.; Hasan, A.; Das, S.; Ahmed, R.; Mori, Y.; Notomi, T.; Kevadiya, B. D.; Thakor, A. S. Loop-mediated isothermal amplification (LAMP): A rapid, sensitive, specific, and cost-effective point-of-care test for coronaviruses in the context of COVID-19 pandemic. Biology (Basel). 2020, 9, 182.
Lalli, M. A.; Langmade, S. J.; Chen, X.; Fronick, C. C.; Sawyer, C. S.; Burcea, L. C.; Wilkinson, M. N.; Fulton, R. S.; Heinz, M.; Buchser, W. J. et al. Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification. Clin. Chem. 2021, 67, 415–424.
Fozouni, P.; Son, S.; Derby, M. D. d. L.; Knott, G. J.; Gray, C. N.; D'Ambrosio, M. V.; Zhao, C.; Switz, N. A.; Kumar, G. R.; Stephens, S. I. et al. Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy. Cell 2021, 184, 323–333.e9.
Ding, X.; Yin, K.; Li, Z.; Lalla, R. V.; Ballesteros, E.; Sfeir, M. M.; Liu, C. Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay. Nat. Commun. 2020, 11, 4711.
Shen, M.; Zhou, Y.; Ye, J.; Al-Maskri, A. A. A.; Kang, Y.; Zeng, S.; Cai, S. Recent advances and perspectives of nucleic acid detection for coronavirus. J. Pharm. Anal. 2020, 10, 97–101.
Chen, M.; Cui, D.; Zhao, Z.; Kang, D.; Li, Z.; Albawardi, S.; Alsageer, S.; Alamri, F.; Alhazmi, A.; Amer, M. R. et al. Highly sensitive, scalable, and rapid SARS-CoV-2 biosensor based on In2O3 nanoribbon transistors and phosphatase. Nano. Res. 2022, 15, 5510–5516.
Hu, R.; Liao, T.; Ren, Y.; Liu, W.; Ma, R.; Wang, X.; Lin, Q.; Wang, G.; Liang, Y. Sensitively detecting antigen of SARS-CoV-2 by NIR-II fluorescent nanoparticles. Nano Res. 2022, 15, 7313–7319.
Deeks, J. J.; Dinnes, J.; Takwoingil, Y.; Davenport, C.; Spijker, R.; Taylor-Phillips, S.; Adrianol, A.; Beesel, S.; Dretzkel, J.; di Ruffanol, L. F. et al. Antibody tests for identification of current and past infection with SARS-CoV-2. Cochrane Db. Syst. Rev. 2020, 6, CD013652.
Liu, W.; Liu, L.; Kou, G.; Zheng, Y.; Ding, Y.; Ni, W.; Wang, Q.; Tan, L.; Wu, W.; Tang, S. et al. Evaluation of nucleocapsid and spike protein-based enzyme-linked immunosorbent assays for detecting antibodies against SARS-CoV-2. J. Clin. Microbiol. 2020, 58, e00461–20.
Scohy, A.; Anantharajah, A.; Bodeus, M.; Kabamba-Mukadi, B.; Verroken, A.; Rodriguez-Villalobos, H. Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis. J. Clin. Virol. 2020, 129, 104455.
Dinnes, J.; Deeks, J. J.; Adriano, A.; Berhane, S.; Davenport, C.; Dittrich, S.; Emperador, D.; Takwoingi, Y.; Cunningham, J.; Beese, S. et al. Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection (Review). Cochrane Db. Syst. Rev. 2020, 8, CD013705.
Reen, D. J. Enzyme-linked immunosorbent assay (ELISA). Methods. Mol. Bioll. 1994, 32, 461–466.
Zhang, D.; Li, W.; Ma, Z.; Han, H. Improved ELISA for tumor marker detection using electro-readout-mode based on label triggered degradation of methylene blue. Biosens. Bioelectron. 2019, 126, 800–805.
Wu, L.; Li, G.; Xu, X.; Zhu, L.; Huang, R.; Chen, X. Application of nano-ELISA in food analysis: Recent advances and challenges. Trac-Trend Anal. Chem. 2019, 113, 140–156.
de la Rica, R.; Stevens, M. M. Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye. Nat. Nanotechnol. 2012, 7, 821–824.
Luo, Q.; Wang, H.; Yin, X.; Wang, L. Hydrophilic perovskite microdisks with excellent stability and strong fluorescence for recyclable temperature sensing. Sci. China. Mater. 2019, 62, 1065–1070.
Chu, B.; Song, B.; Ji, X.; Su, Y.; Wang, H.; He, Y. Fluorescent silicon nanorods-based ratiometric sensors for long term and real-time measurements of intracellular pH in live cells. Anal. Chem. 2017, 89, 12152–12159.
Zhai, X.; Song, B.; Chu, B.; Su, Y.; Wang, H.; He, Y. Highly fluorescent, photostable, and biocompatible silicon theranostic nanoprobes against Staphylococcus aureus infections. Nano Res. 2018, 11, 6417–6427.
Guo, D.; Ji, X.; Peng, F.; Zhong, Y.; Chu, B.; Su, Y.; He, Y. Photostable and biocompatible fluorescent silicon nanoparticles for imaging-guided co-delivery of siRNA and doxorubicin to drug-resistant cancer cells. Nano-micro. Lett. 2019, 11, 27.
Sun, J.; Hu, T.; Chen, C.; Zhao, D.; Yang, F.; Yang, X. Fluorescence immunoassay system via enzyme-enabled in situ synthesis of fluorescent silicon nanoparticles. Anal. Chem. 2016, 88, 9789–9795.
Chen, C.; Zhao, D.; Wang, B.; Ni, P.; Jiang, Y.; Zhang, C.; Yang, F.; Lu, Y.; Sun, J. Alkaline phosphatase-triggered in situ formation of silicon-containing nanoparticles for a fluorometric and colorimetric dual-channel immunoassay. Anal. Chem. 2020, 92, 4639–4646.
Wang, J.; Liu, X.; Huang, L.; Jin, J.; Jiang, C.; Li, D.; Wen, H.; Hu, J. Controllable and robust dual-emissive quantum dot nanohybrids as inner filter-based ratiometric probes for visualizable melamine detection. Nanoscale 2020, 12, 4562–4572.
Zhang, J.; Qian, J.; Mei, Q.; Yang, L.; He, L.; Liu, S.; Zhang, C.; Zhang, K. Imaging-based fluorescent sensing platform for quantitative monitoring and visualizing of fluoride ions with dual-emission quantum dots hybrid. Biosens. Bioelectron. 2019, 128, 61–67.
Zhang, Y.; Guo, S.; Jiang, Z.; Mao, G.; Ji, X.; He, Z. Rox-DNA functionalized silicon nanodots for ratiometric detection of mercury ions in live cells. Anal. Chem. 2018, 90, 9796–9804.
京公网安备11010802044758号