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Research Article

Electrochemical biosensor employing PbS colloidal quantum dots/Au nanospheres-modified electrode for ultrasensitive glucose detection

Yunong Zhao§, Jing Huang§, Qing Huang, Yanbing Tao, Ruiqin Gu, Hua-Yao Li, Huan Liu( )
School of Optical and Electronic Information, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China

§ Yunong Zhao and Jing Huang contributed equally to this work.

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Graphical Abstract

We demonstrate a sensitive glucose electrochemical biosensor through the synergetic labelling strategy utilizing PbS colloidal quantum dots (CQDs) and Au nanospheres (AuNSs). The electrochemical biosensor employing PbS CQDs/AuNSs/GOx-modified electrode is capable of transducing the glucose enzyme-catalyzed reaction into significant current signals. The glucose sensor exhibits good linear response in the concentration range of 0.1 μM–10 mM, and the limit of detection (LOD) is as low as 1.432 nM.

Abstract

Rapid and accurate detection of glucose is of great significance for diabetic management. Highly sensitive glucose sensors promise to achieve noninvasive detection technology, enabling more convenient and efficient means for large-scale screening and long-term dynamic monitoring of diabetes patients. In this work, we demonstrate a sensitive glucose electrochemical biosensor through the synergetic labelling strategy utilizing PbS colloidal quantum dots (CQDs) and Au nanospheres (AuNSs). The PbS CQDs/AuNSs/glucose oxidase (GOx) mixture could be stably immobilized on the carbon electrode surface via the one-step dip-coating method. The electrochemical biosensor employing PbS CQDs/AuNSs/GOx-modified electrode integrates the functions of specific molecule recognition, signal transduction as well as signal amplification. The sensor is capable of transducing the glucose enzyme-catalyzed reaction into significant current signals, exhibiting a good linear response in the glucose concentration range of 0.1 μM–10 mM with the limit of detection being 1.432 nM.

Keywords

colloidal quantum dotgold nanosphereligand exchangeelectrochemicalglucose biosensorelectronic labelling

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Nano Research
Volume 16 Issue 3,
March 2023
Pages 4085-4092
DOI: 10.1007/s12274-022-5138-0
Cite this article:
Zhao Y, Huang J, Huang Q, et al. Electrochemical biosensor employing PbS colloidal quantum dots/Au nanospheres-modified electrode for ultrasensitive glucose detection. Nano Research, 2023, 16(3): 4085-4092. https://doi.org/10.1007/s12274-022-5138-0
Topics:
Biosensors
Part of a topical collection:
Women’s Special Issue: Nanomaterials: Preparation, Applications, and ChallengesWomen’s special issuse in nanomaterials

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Received: 30 June 2022
Revised: 16 September 2022
Accepted: 03 October 2022
Published: 09 November 2022
© Tsinghua University Press 2022
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