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Research Article

A self-activated NO-releasing hydrogel depot for photothermal enhanced sterilization

Shen Zhang1, Kelei Guan3, Yaoxin Zhang1, Junqing Zhang1, Hongyu Fu4, Ting Wu2, Dilan Ouyang2, Chaoqun Liu1( ), Qiang Wu1( ), Zhaowei Chen2( )
School of Pharmacy, Henan University, Kaifeng 475004, China
Institute of Food Safety and Environment Monitoring, College of Chemistry, Fuzhou University, Fuzhou 350108, China
Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Henan University School of Stomatology, Kaifeng 475004, China
Show Author Information

Graphical Abstract

Here, a self-activated NO-releasing hydrogel depot is constructed, which can release NO continuously at the infection site via cascading chemical reactions between CaO2, H2O, and L-arginine. By combining with the photothermal effect of oxide mesoporous carbon nanoparticles, the hydrogel depot possesses a synergistic sterilization behavior both in vitro and in vivo.

Abstract

The ascendant danger of bacterial infections has resulted in an urgent requirement for developing new antibacterial approaches. Recently, nitric oxide (NO) has shown a broad-spectrum antimicrobial activity while avoiding resistance. However, achieving effective NO-based antibacterial therapies remains challenging, mired by the safety concerns of NO donor, residual toxicity by the ingredients, or complicated procedures. Herein, a self-activated NO-releasing hydrogel depot is fabricated by Ca2+-crosslinked sodium alginate doped with CaO2 nanoparticles, L-arginine, and oxidized mesoporous carbon nanoparticles (OMCN) for photothermal enhanced bacteria killing. The locally concentrated H2O2, generated from the reaction of CaO2 nanoparticles and water, could oxidize L-arginine to release NO. Meanwhile, benefiting from the remarkable photothermal effect of OMCN, the hybrid hydrogel possesses a synergistic sterilization behavior in combating both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria in vitro. Moreover, the dual therapeutic hydrogel displays high efficiency in treatment of bacteria-infected mice with back wound model while showing no distinct toxicity. In addition, the restricted environment of hydrogel makes it easy to remove all the components from the infected wound, alleviating possible side effects from exogenous H2O2. As such, the designed NO-synergistic photothermal hydrogel provides a promising strategy for treating bacterial infections.

Keywords

bacterial infectionsnitric oxidehydrogelphotothermalantibacterial

Electronic Supplementary Material

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Nano Research
Volume 16 Issue 4,
April 2023
Pages 5346-5356
DOI: 10.1007/s12274-022-5239-9
Cite this article:
Zhang S, Guan K, Zhang Y, et al. A self-activated NO-releasing hydrogel depot for photothermal enhanced sterilization. Nano Research, 2023, 16(4): 5346-5356. https://doi.org/10.1007/s12274-022-5239-9
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Received: 20 September 2022
Revised: 20 October 2022
Accepted: 23 October 2022
Published: 31 December 2022
© Tsinghua University Press 2022
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