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Research Article

Trojan nanobacteria hybridized with prodrug nanocapsules for efficient combined tumor therapy

Mingsong Zang1, Yuancheng Ji1, Xiaoran Ding2, Zhengwei Xu2, Jinxing Hou1, Jianxin Sun1, Jiayun Xu2, Shuangjiang Yu2( ), Hongcheng Sun2( ), Tingting Wang3( ), Junqiu Liu1,2( )
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China
Key Laboratory of Organosilicon Chemistry and Material Technology of Ministry of Education, Key Laboratory of Organosilicon Material Technology of Zhejiang Province, College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117583, Singapore
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Graphical Abstract

This hybrid of living microorganisms and non-living objects with both the versatility of nanocapsules (chemotherapeutic, photothermal therapy (PTT), and chemodynamic therapy (CDT)) and the active tumor targeting of bacteria has tremendous potential as an antitumor platform.

Abstract

Live bacteria-based drug delivery systems have been raised as promising tools for enhancing drug delivery into tumors due to their active tumor targeting and easy surface modifiability. In this work, a “Trojan nanobacteria hybrid”, E. coli@highly integrated nanocapsules (HINCs) hybrid (HINE-Hybrid), was successfully constructed with HINCs of prodrug based on covalent self-assembly and the facultative anaerobic bacterium E. coli MG 1655 for combined chemotherapy, photothermal therapy (PTT), and chemodynamic therapy (CDT). HINCs were constructed by covalent cross-linking of pillar[5]arene derivatives and cisplatin prodrug linker, which can be endocytosed and lysed to release therapeutic agents. Under the near-infrared (NIR) light (at 808 nm) irradiation, the system temperature can be significantly increased by HINCs, which further leads to the highly efficient generation of reactive oxygen species (ROS). In addition, HINE-Hybrid shows significant antitumor effects in in vitro and in vivo studies and also promotes immune cell infiltration and antitumor cytokine expression in the tumor microenvironment (TME). HINE-Hybrid exerts its anticancer properties efficiently due to selective enrichment and multiplication of E. coli at tumor sites, which is important for the construction of bacterial-assisted antitumor platforms.

Keywords

live bacteriahybridcovalent nanocapsulephotothermal therapychemodynamic therapy

Electronic Supplementary Material

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Nano Research
Volume 16 Issue 7,
July 2023
Pages 9651-9662
DOI: 10.1007/s12274-023-5503-7
Cite this article:
Zang M, Ji Y, Ding X, et al. Trojan nanobacteria hybridized with prodrug nanocapsules for efficient combined tumor therapy. Nano Research, 2023, 16(7): 9651-9662. https://doi.org/10.1007/s12274-023-5503-7
Topics:
BiocompatibilityBiomaterials

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Received: 26 December 2022
Revised: 10 January 2023
Accepted: 11 January 2023
Published: 20 April 2023
© Tsinghua University Press 2023
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