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Research Article

β-Cyclodextrin-modified AuBi metallic aerogels enable efficient peroxidase mimicking for colorimetric sensing of urease-positive pathogenic bacteria

Minghui Wang, Peixian Wu, Sha Yang, Gui-long Wu, Na Li, Xiaofeng Tan( ), Qinglai Yang( )
Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang 421001, China
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Graphical Abstract

β-Cyclodextrin/AuBi aerogels are prepared based on the surface modification and one-pot reduction strategy. The introduction of β-cyclodextrin (featured with a hydrophobic cavity and hydrophilic surface) could effectively boost the catalytic activity of AuBi aerogels by engendering host–guest complex and improving dispersity/stability. By utilizing the specific urea hydrolysis by urease for producing NH3 to raise pH, the pH up-regulation would inhibit the peroxidase-mimicking performances of β-cyclodextrin/AuBi aerogels. Therefore, the sensitive colorimetric detection platform for urease activity, urease-positive P. mirabilis, and fluoride ion could be constructed.

Abstract

The detection of pathogenic bacteria with improved accessibility, reduced analysis time, and increased sensitivity is of great importance for diagnosing the infected disease. Nanozymes have attracted rising attention in the bioassay field. Designing a model nanozyme needs the combined merit of sensible nanostructures and a large specific surface area to guarantee exceptional enzyme-mimic activity. Herein, a β-cyclodextrin modified AuBi aerogel is prepared by a one-pot reduction strategy. The introduction of β-cyclodextrin (featured with a hydrophobic cavity and hydrophilic surface) enhances the catalytic activity of AuBi aerogels by engendering host–guest complex and improving dispersity/stability. Based on the specific urea hydrolysis, which could produce NH3 to raise pH by urease, the pH up-regulation would inhibit the peroxidase-mimicking performances of β-cyclodextrin/AuBi aerogels. Therefore, the sensitive colorimetric detection platform for urease activity could be constructed. Moreover, the sensing platform can detect straightforwardly urease-positive Proteus mirabilis in urine circumstances with a wide detection range and a low limit of detection (LOD) of 4 colony-forming unit (CFU)·mL−1. The reproducibility, stability, and specificity of this approach are verified to be satisfactory. Also, as an inhibitor of urease activity, the fluoride ion could be detected by the constructed sensing platform sensitively and specifically. Overall, this work provides a blueprint for designing an ideal nanozyme and paves a new roadway for detecting pathogenic bacteria.

Keywords

metallic aerogelssurface modificationurea hydrolysisurease activityfluoride ionproteus mirabilisbacteria detection

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Nano Research
Volume 16 Issue 7,
July 2023
Pages 9663-9671
DOI: 10.1007/s12274-023-5519-z
Cite this article:
Wang M, Wu P, Yang S, et al. β-Cyclodextrin-modified AuBi metallic aerogels enable efficient peroxidase mimicking for colorimetric sensing of urease-positive pathogenic bacteria. Nano Research, 2023, 16(7): 9663-9671. https://doi.org/10.1007/s12274-023-5519-z
Topics:
Bacteria Nanosensors

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Received: 30 November 2022
Revised: 07 January 2023
Accepted: 18 January 2023
Published: 13 April 2023
© Tsinghua University Press 2023
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