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Research Article

A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics

Xianlu Lei1,2, Xinxin Xu1,2( ), Li Wang1,2, Wei Zhou3, Liqiang Liu1,2, Liguang Xu1,2, Hua Kuang1,2( ), Chuanlai Xu1,2
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Jiangsu Product Quality Testing and Inspection Institute, Nanjing 210000, China
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Graphical Abstract

Four test lines were setup in colloidal gold immunochromatographic strip for high throughput detection of anesthetics in fish. A portable reader makes the visualized color change into quantitative data.

Abstract

Anesthetic residues in fish represent a potential risk to human health. Therefore, it is important to develop a sensitive and broad-specific method for the detection of anesthetics. In this study, we developed a colloidal gold-based quadruplex immunochromatographic (Qua-ICS) assay using four highly sensitive monoclonal antibody immunotherapy (mAbs) that simultaneously detected 11 anesthetic residues in fish within 10 min. The colorimetric and cut-off values (COVs) for procaines, eugenols, bupivacaines, and tricaine (TMS) were 0.37–1.1 and 3.3–10, 11–222 and 100–2000, 0.37 and 3.3, and 111 and 10,000 µg/kg, respectively. Quantitative analysis was achieved with a portable strip-reader, and the detection ranges were 0.15–2.6, 6.3–677, 0.13–2.8, and 83–1245 µg/kg for procaines, eugenols, bupivacaines, and tricaine, respectively. Our developed method was reliable and accurate according to the recovery test results and analyses of real samples. Therefore, the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.

Keywords

bupivacainecolloidal goldeugenolprocainetricaine (TMS)

Electronic Supplementary Material

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Nano Research
Volume 16 Issue 8,
August 2023
Pages 11269-11277
DOI: 10.1007/s12274-023-5768-x
Cite this article:
Lei X, Xu X, Wang L, et al. A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics. Nano Research, 2023, 16(8): 11269-11277. https://doi.org/10.1007/s12274-023-5768-x
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Chemical sensors

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Received: 04 February 2023
Revised: 13 April 2023
Accepted: 23 April 2023
Published: 13 June 2023
© Tsinghua University Press 2023
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