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Research Article

Time-resolved luminescent nanoprobes based on lanthanide nucleotide self-assemblies for alkaline phosphatase detection

Yunqin Zhang1,2,§, Yang Cao1,4,5,§, Yan Liu1,2,5( ), Yingjie Yang1, Mingmao Chen3, Hang Gao1, Lushan Lin1, Xueyuan Chen1,2,4,5( )
CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Key Laboratory of Nanomaterials, and State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China
Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou 350108, China
College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350117, China
Fujian College, University of Chinese Academy of Sciences, Fuzhou 350002, China

§ Yunqin Zhang and Yang Cao contributed equally to this work.

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Graphical Abstract

A novel strategy for reliable and accurate detection of an enzyme disease biomarker alkaline phosphatase (ALP) was developed by utilizing lanthanide (Ln3+) nucleotide nanoparticles (LNNPs) with extremely improved monodispersity and uniformity, which were achieved by restricting the ATP-Ln3+ self-assembly inside micellar nanoreactor.

Abstract

Currently, enzyme-responsive nanomaterials have shown great promise in prognosis or diagnosis of disease biomarker. However, the great obstacle for conventional enzyme-responsive nanomaterials frequently lies in autofluorescence interference, poor monodispersity, uncontrollable size and morphology, low optical stability, and biotoxicity, which fundamentally impede their practical application in biological systems. To overcome these deficiencies, we proposed a novel strategy for reliable and precise detection of an enzyme disease biomarker, alkaline phosphatase (ALP), through lanthanide (Ln3+) nucleotide nanoparticles (LNNPs) with extremely improved monodispersity and uniformity, which were achieved by the coordination self-assembly between ATP and Ln3+ inside micellar nanoreactor. Specifically, for ATP-Ce/Tb LNNPs, highly improved photoluminescence (PL) emission of Tb3+ can be achieved via efficient Ce3+ sensitization. We demonstrated that ALP could specifically cleave the phosphorus–oxygen (P–O) bonds of ATP and result in the collapse of ATP-Ce/Tb scaffold, finally leading to the PL quenching of Tb3+. By taking advantage of time-resolved (TR) PL technique, the fabricated ATP-Ce/Tb LNNPs presented superior selectivity and sensitivity for the ALP bioassay in complicated serum samples, thus revealing the great potential of ATP-Ce/Tb LNNPs in the areas of ALP-related disease prognosis and diagnosis.

Keywords

alkaline phosphatase (ALP)-responsivemicellar nanoreactorcoordination self-assemblylanthanide sensitizationtime-resolved photoluminescence (TRPL)

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Nano Research
Volume 16 Issue 8,
August 2023
Pages 11250-11258
DOI: 10.1007/s12274-023-5863-z
Cite this article:
Zhang Y, Cao Y, Liu Y, et al. Time-resolved luminescent nanoprobes based on lanthanide nucleotide self-assemblies for alkaline phosphatase detection. Nano Research, 2023, 16(8): 11250-11258. https://doi.org/10.1007/s12274-023-5863-z
Topics:
Nano detectionNanoprobes

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Received: 03 April 2023
Revised: 10 May 2023
Accepted: 21 May 2023
Published: 15 July 2023
© Tsinghua University Press 2023
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