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Research Article

Assembly of highly efficient aqueous light-harvesting system from sequence-defined peptoids for cytosolic microRNA detection

Yang Song1,2, Xiaoli Cai1, Mingming Wang2, Dan Du1, Yuehe Lin1( ), Chun-Long Chen2,3( )
Department of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Division of Physical Sciences, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
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Graphical Abstract

Self-assembly of sequence-defined peptoids into crystalline nanotubes enabled the creation of a highly efficient artificial light-harvesting system that can sensitively detect microRNA within cells.

Abstract

Precisely controlled spatial distributions of artificial light-harvesting systems in aqueous media are of significant importance for mimicking natural light-harvesting systems; however, they are often restrained by the solubility and the aggregation-caused quenching effect of the hydrophobic chromophores. Herein, we report one highly efficient artificial light-harvesting system based on peptoid nanotubes that mimic the hierarchical cylindrical structure of natural systems. The high crystallinity of these nanotubes enabled the organization of arrays of donor chromophores with precisely controlled spatial distributions, favoring an efficient Förster resonance energy transfer (FRET) process in aqueous media. This FRET system exhibits an extremely high efficiency of 98.6% with a fluorescence quantum yield of 40% and an antenna effect of 29.9. We further demonstrated the use of this artificial light-harvesting system for quantifying miR-210 within cancer cells. The fluorescence intensity ratio of donor to acceptor is linearly related to the concentration of intercellular miR-210 in the range of 3.3–156 copies/cell. Such high sensitivity in intracellular detection of miR-210 using this artificial light-harvesting system offers a great opportunity and pathways for biological imaging and detection, and for the further creation of microRNA (miRNA) toolbox for quantitative epigenetics and personalized medicine.

Keywords

light-harvestingpeptoid nanotubeFörster resonance energy transferintracellular RNA detection

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Nano Research
Volume 17 Issue 2,
February 2024
Pages 788-796
DOI: 10.1007/s12274-023-6008-0
Cite this article:
Song Y, Cai X, Wang M, et al. Assembly of highly efficient aqueous light-harvesting system from sequence-defined peptoids for cytosolic microRNA detection. Nano Research, 2024, 17(2): 788-796. https://doi.org/10.1007/s12274-023-6008-0
Topics:
Biotechnology
Part of a topical collection:
NR45 Awards in Bio-inspired Nanomaterials, 2023

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Received: 11 May 2023
Revised: 07 July 2023
Accepted: 15 July 2023
Published: 20 August 2023
© Tsinghua University Press 2023
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