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Research Article

Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment

Xue Mao1,§, Zhen-Zhen Li3,§, Dong-Lian Hao1, Wei-Dong Han4, Gao-Peng Li2( ), Yao-Yu Wang5, Kun Zhang1( )
School of Textile Science and Engineering, Key Laboratory of Functional Textile Material and Product (Ministry of Education), Xi’an Polytechnic University, Xi’an 710048, China
Key Laboratory of Magnetic Molecules & Magnetic Information Materials of the Ministry of Education, School of Chemistry & Material Science, Shanxi Normal University, Taiyuan 030031, China
Department of Clinical Laboratory, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
Shanghai iProteome Biotechnology Co., Ltd, Shanghai 201210, China
College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China

§ Xue Mao and Zhen-Zhen Li contributed equally to this work.

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Graphical Abstract

Biomimetic design of highly flexible and mechanical robust metal oxide TiO2@ZrTiO4 nanofibrous membranes with branch microstructure afford superior phosphopeptides enrichment and phosphoproteins identification property for cancer cell.

Abstract

Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches, but still a significant challenge in materials design. To this end, we firstly constructed highly flexible ZrTiO4 nanofibrous membranes (NFMs) with excellent mechanical stability through a cost-effective and scalable electrospinning and subsequent calcination technique. Then, to further increase the enrichment capacity of the phosphopeptide, the biomimetic TiO2@ZrTiO4 NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO4 NFMs through growing unfurling TiO2 nanosheets onto the ZrTiO4 nanofibers. Importantly, remarkable flexibility and mechanical stability enable the resulting TiO2@ZrTiO4 NFMs excellent practicability, while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer cells. Specifically, 6770 phosphopeptides can be enriched by TiO2@ZrTiO4 NFMs (2205 corresponding phosphoproteins can be identified), and the value is much higher than that of ZrTiO4 NFMs (6399 phosphopeptides and 2132 identified phosphoproteins) and commercial high-performance TiO2 particles (4525 phosphopeptides and 1811 identified phosphoproteins). These results demonstrate the super ability of TiO2@ZrTiO4 NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.

Keywords

inorganic nanofibersflexible ceramic nanofibrous membranesbiomimetic materialsexceptional mechanical performanceflexibility

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Nano Research
Volume 17 Issue 5,
May 2024
Pages 4270-4278
DOI: 10.1007/s12274-023-6277-7
Cite this article:
Mao X, Li Z-Z, Hao D-L, et al. Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment. Nano Research, 2024, 17(5): 4270-4278. https://doi.org/10.1007/s12274-023-6277-7
Topics:
ElectrospinningNanofibersOxides

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Received: 04 September 2023
Revised: 09 October 2023
Accepted: 18 October 2023
Published: 01 December 2023
© Tsinghua University Press 2023
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