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Research Article

Boosting solar hydrogen production via electrostatic interaction mediated E. coli-TiO2−x biohybrid system

Xingxing Lv1,2, Weicheng Huang3, Ya Gao1, Rui Chen1, Xiaowei Chen3, Danqing Liu1( ), Ling Weng1( ), Liangcan He2,4( ), Shaoqin Liu2,4
School of Material Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150040, China
School of Medicine and Health, and Key Laboratory of Micro-systems and Micro-structures Manufacturing of Ministry of Education, Harbin Institute of Technology, Harbin 150001, China
Department of Physics, School of Science, Jimei University, Xiamen 361021, China
Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou 450046, China
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Graphical Abstract

An intracellular inorganic biohybrid system was designed and synthesized. Oxygen-deficient titanium dioxide (TiO2−x) was combined with Escherichia coli through electrostatic interaction. This biohybrid system exploits both the light-absorbing properties of the material and combines the efficient catalytic ability of living microorganisms in a clean and selective manner, providing a new viable pathway for solar-to-chemical conversion.

Abstract

Hydrogen is garnering growing attention as a green energy source with zero carbon emissions. However, most hydrogen production technologies still rely on the consumption of fossil fuels and are therefore unsustainable. This has driven the search for more environmentally friendly methods of hydrogen production. In this work, we present an innovative approach to enhance hydrogen generation via electrostatic interaction in the Escherichia coli and defective titanium dioxide (TiO2−x) biohybrids. Our method involves narrowing the forbidden bandwidth of TiO2 while introducing defect bands into its conduction band to facilitate visible light absorption and efficient charge separation. This biohybrid system, consisting of E. coli and TiO2−x, demonstrates a remarkable capability to produce 1.25 mmol of hydrogen within a 3-h timeframe under visible light irradiation. This accomplishment signifies a 3.31-fold rise in hydrogen production in comparison to E. coli, signifying a substantial enhancement in hydrogen production efficiency. Furthermore, we delve into the alterations in biological metabolites associated with hydrogen production and the changes in electron transfer in different biohybrid systems. It provides valuable insights into the understanding of the intrinsic mechanisms that drive the process. This work introduces a novel and promising avenue for achieving this exciting goal.

Keywords

Escherichia coliTiO2−x nanoparticlesbiohybrid systemhydrogen production

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Nano Research
Volume 17 Issue 6,
June 2024
Pages 5390-5398
DOI: 10.1007/s12274-024-6432-9
Cite this article:
Lv X, Huang W, Gao Y, et al. Boosting solar hydrogen production via electrostatic interaction mediated E. coli-TiO2−x biohybrid system. Nano Research, 2024, 17(6): 5390-5398. https://doi.org/10.1007/s12274-024-6432-9
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Received: 19 November 2023
Revised: 13 December 2023
Accepted: 20 December 2023
Published: 25 January 2024
© Tsinghua University Press 2024
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