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Cellular uptake and cytotoxicity. (a) Confocal microscope images of 4T1 cells incubated with DOX solution or prodrug ANPs for 0.5, 2 and 4 h. Scale bar: 20 μm. IC50 values (nmol/L) of DOX solution, DOX-ANPs and prodrug ANPs against (b) 4T1 cells, (c) KB cells, (d) Hepa 1-6 cells, and (e) L02 cells (n = 3). Data are presented as means ± S.D. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.001 by two-tailed Student’s t-test.
Cellular uptake and cytotoxicity. (a) Confocal microscope images of 4T1 cells incubated with DOX solution or prodrug ANPs for 0.5, 2 and 4 h. Scale bar: 20 μm. IC50 values (nmol/L) of DOX solution, DOX-ANPs and prodrug ANPs against (b) 4T1 cells, (c) KB cells, (d) Hepa 1-6 cells, and (e) L02 cells (n = 3). Data are presented as means ± S.D. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.001 by two-tailed Student’s t-test.