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Research Article

Enhancing osteogenic bioactivities of coaxial electrospinning nano-scaffolds through incorporating iron oxide nanoparticles and icaritin for bone regeneration

Peng Wang1,2,4,§, Qianjin Wang2,§, Dengxian Wu2,§, Yunyang Zhang3, Shixiong Kang1, Xucai Wang5, Jiayu Gu6, Hao Wu1( ), Zhihong Xu2( ), Qing Jiang2( )
School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, China
State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Center of Modem Analysis, Nanjing University, Nanjing 210008, China
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210009, China
Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Jiangsu Institute of Metrology, Nanjing 210023, China

§ Peng Wang, Qianjin Wang, and Dengxian Wu contributed equally to this work.

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Graphical Abstract

Iron oxide nanoparticles (IONPs) and icaritin (ICA) incorporated electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for the treatment of bone defects.

Abstract

Bone tissue engineering provides a promising strategy for the treatment of bone defects. Nonetheless, the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and absence of osteo-inductive properties. Here, we proposed to endow nano-scaffold (NS) constructed by coaxial electrospinning technique with enhanced osteogenic bioactivities and mechanical properties by incorporating biocompatible magnetic iron oxide nanoparticles (IONPs) and icaritin (ICA). Four types of nano-scaffolds (NS, ICA@NS, NS-IONPs and ICA@NS-IONPs) were prepared. The incorporation of ICA and IONPs minimally impact their surface morphological and chemical properties. IONPs enhanced the mechanical properties of NS scaffolds, including hardness, tensile strength, and elastic modulus. In vitro assessments demonstrated that ICA@NS-IONPs exhibited enhanced osteogenic bioactivities towards mouse calvarial pre-osteoblast cell line MC3T3-E1 as evidenced by detecting the alkaline phosphatase (ALP) activity level, expressions of osteogenesis-related genes and proteins as well as mineralized nodule formation. Mechanistic investigations revealed that MEK/ERK (MAP kinase-ERK kinase (MEK)/extracellular-signal-regulated kinase (ERK)) signaling pathway could offer a plausible explanation for the osteogenic differentiation of MC3T3-E1 cells induced by ICA@NS-IONPs. Furthermore, the implantation of nano-scaffolds in rat skull defects exhibited a substantial improvement in in vivo bone regeneration. Therefore, IONPs and ICA incorporated coaxial electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for bone tissue engineering.

Keywords

iron oxide nanoparticlesicaritincoaxial electrospinning nano-scaffoldsMEK/ERK signaling pathwaybone regeneration

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Nano Research
Volume 17 Issue 7,
July 2024
Pages 6430-6442
DOI: 10.1007/s12274-024-6656-8
Cite this article:
Wang P, Wang Q, Wu D, et al. Enhancing osteogenic bioactivities of coaxial electrospinning nano-scaffolds through incorporating iron oxide nanoparticles and icaritin for bone regeneration. Nano Research, 2024, 17(7): 6430-6442. https://doi.org/10.1007/s12274-024-6656-8
Topics:
Tissue engineering

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Received: 15 October 2023
Revised: 25 March 2024
Accepted: 25 March 2024
Published: 08 May 2024
© Tsinghua University Press 2024
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