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Research Article

Enhancing the peroxidase-like activity of MoS2-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione

Feng Feng1, Yihe Zhang1( ), Xiao Zhang1, Bin Mu2, Jiahe Zhang1, Wenjie Qu1, Wangshu Tong1, Minmin Liang3, Qi An1, Zhanjun Guo3( ), Lu Zhao1( )
Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources, Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing 100083, China
Key Laboratory of Clay Mineral Applied Research of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
Experimental Center of Advanced Materials School of Materials Science and Engineering, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
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Graphical Abstract

This work presents a composite nanozyme (Fe-ATP-MoS2) based on attapulgite, which exhibits significantly enhanced peroxidase (POD)-like activity. The introduction of attapulgite increases the electron density of metal centers, and improves substrate binding affinities, thus advancing the POD-like activity. The developed nanozyme demonstrates promising applications in the antibacterial and sensitive detection of glutathione.

Abstract

Nanozymes are next-generation of nanomaterials with enzyme-like activities. In particular, nanozymes with peroxidase (POD)-like activity have been utilized in various fields, including antibacterial, detection, degradation, etc. However, their extensive applications were limited by their low catalytic activity currently. Herein, we have presented a composite nanozyme based on attapulgite (ATP) (Fe-ATP-MoS2 (FAM)), which exhibited enhanced POD-like activity (185.33 U·mg−1), 4.25 times higher than that of Fe-MoS2 (FM) (43.63 U·mg−1). The density functional theory (DFT) calculations indicated that the addition of ATP increased the electron density of metal centers (Mo and Fe). More importantly, Michaelis–Menten kinetics revealed that the introduction of ATP significantly enhanced the binding affinities of substrates through the pores of ATP, forming a highly concentrated substrate microenvironment and thus promoting its POD-like activity. Additionally, from molecular size and kinetic analysis, we proposed that the changes in substrate size before and after oxidation also significantly affected its Michaelis-constant (Km) value. Furthermore, we utilized FAM in the applications of highly effective antibacterial application and sensitive detection of glutathione (GSH). In conclusion, this work provides a novel approach for designing a highly efficient nanozyme based on natural mineral composites.

Keywords

attapulgiteperoxidaseelectron densityaffinityantibacterial

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Nano Research
Volume 17 Issue 8,
August 2024
Pages 7415-7426
DOI: 10.1007/s12274-024-6685-3
Cite this article:
Feng F, Zhang Y, Zhang X, et al. Enhancing the peroxidase-like activity of MoS2-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione. Nano Research, 2024, 17(8): 7415-7426. https://doi.org/10.1007/s12274-024-6685-3
Topics:
Nano biology

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Received: 05 February 2024
Revised: 26 March 2024
Accepted: 04 April 2024
Published: 17 May 2024
© Tsinghua University Press 2024
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