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Research Article

Phenylboronic-tannin nanocolloids that scavenge subchondral reactive oxygen microenvironment and inhibit RANKL induced osteoclastogenesis for osteoarthritis treatment

Xiaoqun Li2, Yufang Kou1, Jia Jia1, Minchao Liu1, Runze Gao2, Yuhong Li2, Gang Li2, Shuogui Xu2, Wei Song3, Yang Xie2( ), Xiaomin Li1( ), Tiancong Zhao1( )
School of Chemistry and Materials, Department of Chemistry, Laboratory of Advanced Materials and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of Molecular Engineering of Polymers, Collaborative Innovation Center of Chemistry for Energy Materials (2011-iChEM), Fudan University, Shanghai 200433, China
Department of Orthopaedics Trauma and Department of Ophthalmology, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China
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Graphical Abstract

A novel poly-tannin-phenylboronic colloidal nanoparticle (PTA) was fabricated, and how PTA clear the receptor activator of nuclear factor-κB ligand (RANKL) signal pathway generated reactive oxygen species (ROS) to inhibit osteoclastogenesis and alleviate osteoarthritis was presented in this work.

Abstract

The excessive reactive oxygen species (ROS) accumulation and overactivated osteoclastogenesis in subchondral bone has proved to be a major cause of osteoarthritis (OA). Scavenging of ROS microenvironment to inhibit the osteoclastogenesis is highly valued in the therapeutic process of osteoarthritis. Despite the excellent ability of polyphenolic colloidal to scavenge reactive oxygen species and its affinity for macrophages, the preparation of polyphenolic colloidal nanoparticles is limited by the complex intermolecular forces between phenol molecules and the lack of understanding of polymerization/sol-gel chemistry. Herein, our work introduces a novel poly-tannin-phenylboronic colloidal nanoparticle (PTA) exclusively linked by ROS-responsive bondings. Nanocolloidal PTA has a uniform particle size, is easy and scalable to synthesize, has excellent scavenging of ROS, and can be slowly degraded. For in vitro experiments, we demonstrated that, PTA could eliminate ROS within RAW264.7 cells and impede osteoclastogenesis and bone resorption. RNA sequencing results of PTA-treated RAW264.7 cells further reveal the promotion of antioxidant activity and inhibition of osteoclastogenesis. For in vivo experiments, PTA could eliminate the ROS environment and reduce the number of osteoclasts in the subchondral bone, thereby alleviating the damage of subchondral bone and symptoms of osteoarthritis. Our research, by delving into the formation of polyphenol colloidal nanoparticles and validating their role in ROS scavenging to inhibit osteoclastogenesis in subchondral bone, may open new avenues for OA treatment in the future.

Keywords

polyphenolnanoparticlereactive oxygen species (ROS)osteoclastogenesissubchondral bone

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Nano Research
Volume 17 Issue 11,
November 2024
Pages 9898-9907
DOI: 10.1007/s12274-024-6891-z
Cite this article:
Li X, Kou Y, Jia J, et al. Phenylboronic-tannin nanocolloids that scavenge subchondral reactive oxygen microenvironment and inhibit RANKL induced osteoclastogenesis for osteoarthritis treatment. Nano Research, 2024, 17(11): 9898-9907. https://doi.org/10.1007/s12274-024-6891-z
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Received: 14 May 2024
Revised: 15 July 2024
Accepted: 15 July 2024
Published: 13 August 2024
© Tsinghua University Press 2024
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