AI Chat Paper
Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
Search articles, authors, keywords, DOl and etc.
{{expandStatus?'Exit ':''}}Advanced Search
Compared with thermodynamically equilibrium supramolecular assemblies, non-equilibrium assemblies from the same building blocks have attracted increasing attentions because their diverse structures and dynamic natures may impart the assemblies with novel functionalities. However, facile access to non-equilibrium assemblies remains a formidable challenge. Herein, we endeavored to exploit various solvent-anti-solvent methods to achieve it using peptide amphiphile C16-VVAAEE-NH2 as a model. Through systematical utilization of dialysis, ultrasonic and stirring-dropping methods, as well as tuning of processing parameters, we demonstrated the successful formation of diverse non-equilibrium nanostructures with distinct morphologies and structures that significantly deviate from the thermodynamically favored twisted long ribbons. Additionally, these metastable nanostructures ultimately underwent spontaneous transformation into thermodynamically stable states. The transformation processes of three representative non-equilibrium assemblies were also demonstrated and characterized in detail using transmission electron microscopy, circular dichroism spectrum, and thioflavin T fluorescence spectrum. Furthermore, non-equilibrium assemblies exhibited various degrees of cytotoxic effects, which may stem from their spontaneous, dynamic transformation and interactions with cellular membranes. This study offers valuable approaches for direct access to diverse non-equilibrium supramolecular nanostructures from self-assembling peptide, and also has implications for the development of advanced materials with unprecedented biological functions.
Whitesides, G. M.; Mathias, J. P.; Seto, C. T. Molecular self-assembly and nanochemistry: A chemical strategy for the synthesis of nanostructures. Science 1991, 254, 1312–1319.
Brizard, A. M.; van Esch, J. H. Self-assembly approaches for the construction of cell architecture mimics. Soft Matter 2009, 5, 1320–1327.
Tao, K.; Makam, P.; Aizen, R.; Gazit, E. Self-assembling peptide semiconductors. Science 2017, 358, eaam9756.
Chagri, S.; Ng, D. Y. W.; Weil, T. Designing bioresponsive nanomaterials for intracellular self-assembly. Nat. Rev. Chem. 2022, 6, 320–338.
Webber, M. J.; Appel, E. A.; Meijer, E. W.; Langer, R. Supramolecular biomaterials. Nat. Mater. 2016, 15, 13–26.
Lampel, A.; Ulijn, R. V.; Tuttle, T. Guiding principles for peptide nanotechnology through directed discovery. Chem. Soc. Rev. 2018, 47, 3737–3758.
Aida, T.; Meijer, E. W.; Stupp, S. I. Functional supramolecular polymers. Science 2012, 335, 813–817.
de Greef, T. F. A.; Meijer, E. W. Supramolecular polymers. Nature 2008, 453, 171–173.
Zhou, X. Q.; Wang, P. Y.; Ramu, V.; Zhang, L. Y.; Jiang, S. H.; Li, X. Z.; Abyar, S.; Papadopoulou, P.; Shao, Y.; Bretin, L. et al. In vivo metallophilic self-assembly of a light-activated anticancer drug. Nat. Chem. 2023, 15, 980–987.
Fukui, T.; Kawai, S.; Fujinuma, S.; Matsushita, Y.; Yasuda, T.; Sakurai, T.; Seki, S.; Takeuchi, M.; Sugiyasu, K. Control over differentiation of a metastable supramolecular assembly in one and two dimensions. Nat. Chem. 2017, 9, 493–499.
Mattia, E.; Otto, S. Supramolecular systems chemistry. Nat. Nanotechnol. 2015, 10, 111–119.
Mann, S. Self-assembly and transformation of hybrid nano-objects and nanostructures under equilibrium and non-equilibrium conditions. Nat. Mater. 2009, 8, 781–792.
Albanese, A.; Tang, P. S.; Chan, W. C. W. The effect of nanoparticle size, shape, and surface chemistry on biological systems. Annu. Rev. Biomed. Eng. 2012, 14, 1–16.
Levin, A.; Hakala, T. A.; Schnaider, L.; Bernardes, G. J. L.; Gazit, E.; Knowles, T. P. J. Biomimetic peptide self-assembly for functional materials. Nat. Rev. Chem. 2020, 4, 615–634.
Cui, H. G.; Webber, M. J.; Stupp, S. I. Self-assembly of peptide amphiphiles: From molecules to nanostructures to biomaterials. Pept. Sci. 2010, 94, 1–18.
Batra, R.; Loeffler, T. D.; Chan, H.; Srinivasan, S.; Cui, H. G.; Korendovych, I. V.; Nanda, V.; Palmer, L. C.; Solomon, L. A.; Fry, H. C. et al. Machine learning overcomes human bias in the discovery of self-assembling peptides. Nat. Chem. 2022, 14, 1427–1435.
Pashuck, E. T.; Cui, H. G.; Stupp, S. I. Tuning supramolecular rigidity of peptide fibers through molecular structure. J. Am. Chem. Soc. 2010, 132, 6041–6046.
Álvarez, Z.; Kolberg-Edelbrock, A. N.; Sasselli, I. R.; Ortega, J. A.; Qiu, R.; Syrgiannis, Z.; Mirau, P. A.; Chen, F.; Chin, S. M.; Weigand, S. et al. Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury. Science 2021, 374, 848–856.
Dehsorkhi, A.; Castelletto, V.; Hamley, I. W. Self-assembling amphiphilic peptides. J. Pept. Sci. 2014, 20, 453–467.
Ozkan, A. D.; Tekinay, A. B.; Guler, M. O.; Tekin, E. D. Effects of temperature, pH and counterions on the stability of peptide amphiphile nanofiber structures. RSC Adv. 2016, 6, 104201–104214.
Panja, S.; Adams, D. J. Stimuli responsive dynamic transformations in supramolecular gels. Chem. Soc. Rev. 2021, 50, 5165–5200.
Korevaar, P. A.; Newcomb, C. J.; Meijer, E. W.; Stupp, S. I. Pathway selection in peptide amphiphile assembly. J. Am. Chem. Soc. 2014, 136, 8540–8543.
Tantakitti, F.; Boekhoven, J.; Wang, X.; Kazantsev, R. V.; Yu, T.; Li, J. H.; Zhuang, E.; Zandi, R.; Ortony, J. H.; Newcomb, C. J. et al. Energy landscapes and functions of supramolecular systems. Nat. Mater. 2016, 15, 469–476.
Kemper, B.; Zengerling, L.; Spitzer, D.; Otter, R.; Bauer, T.; Besenius, P. Kinetically controlled stepwise self-assembly of AuI-metallopeptides in water. J. Am. Chem. Soc. 2018, 140, 534–537.
Singh, A.; Joseph, J. P.; Gupta, D.; Sarkar, I.; Pal, A. Pathway driven self-assembly and living supramolecular polymerization in an amyloid-inspired peptide amphiphile. Chem. Commun. 2018, 54, 10730–10733.
Hickey, J. W.; Santos, J. L.; Williford, J. M.; Mao, H. Q. Control of polymeric nanoparticle size to improve therapeutic delivery. J. Controlled Release 2015, 219, 536–547.
Rao, J. P.; Geckeler, K. E. Polymer nanoparticles: Preparation techniques and size-control parameters. Prog. Polym. Sci. 2011, 36, 887–913.
Kamaly, N.; Yameen, B.; Wu, J.; Farokhzad, O. C. Degradable controlled-release polymers and polymeric nanoparticles: Mechanisms of controlling drug release. Chem. Rev. 2016, 116, 2602–2663.
Molavi, F.; Barzegar-Jalali, M.; Hamishehkar, H. Polyester based polymeric nano and microparticles for pharmaceutical purposes: A review on formulation approaches. J. Controlled Release 2020, 320, 265–282.
Mora-Huertas, C. E.; Fessi, H.; Elaissari, A. Polymer-based nanocapsules for drug delivery. Int. J. Pharm. 2010, 385, 113–142.
Choi, S. W.; Kim, J. H. Design of surface-modified poly(D,L-lactide-co-glycolide) nanoparticles for targeted drug delivery to bone. J. Controlled Release 2007, 122, 24–30.
Cui, H. G.; Cheetham, A. G.; Pashuck, E. T.; Stupp, S. I. Amino acid sequence in constitutionally isomeric tetrapeptide amphiphiles dictates architecture of one-dimensional nanostructures. J. Am. Chem. Soc. 2014, 136, 12461–12468.
Ortony, J. H.; Newcomb, C. J.; Matson, J. B.; Palmer, L. C.; Doan, P. E.; Hoffman, B. M.; Stupp, S. I. Internal dynamics of a supramolecular nanofibre. Nat. Mater. 2014, 13, 812–816.
Berns, E. J.; Sur, S.; Pan, L. L.; Goldberger, J. E.; Suresh, S.; Zhang, S. M.; Kessler, J. A.; Stupp, S. I. Aligned neurite outgrowth and directed cell migration in self-assembled monodomain gels. Biomaterials 2014, 35, 185–195.
Bhardwaj, H.; Jangde, R. K. Current updated review on preparation of polymeric nanoparticles for drug delivery and biomedical applications. Next Nanotechnol. 2023, 2, 100013.
Jeon, H. J.; Jeong, Y. I.; Jang, M. K.; Park, Y. H.; Nah, J. W. Effect of solvent on the preparation of surfactant-free poly(DL-lactide-co-glycolide) nanoparticles and norfloxacin release characteristics. Int. J. Pharm. 2000, 207, 99–108.
Cao, N. N.; Huang, K.; Xie, J. J.; Wang, H.; Shi, X. H. Self-assembly of peptides: The acceleration by molecular dynamics simulations and machine learning. Nano Today 2024, 55, 102160.
Reches, M.; Gazit, E. Formation of closed-cage nanostructures by self-assembly of aromatic dipeptides. Nano Lett. 2004, 4, 581–585.
Cheng, D. B.; Wang, D.; Gao, Y. J.; Wang, L.; Qiao, Z. Y.; Wang, H. Autocatalytic morphology transformation platform for targeted drug accumulation. J. Am. Chem. Soc. 2019, 141, 4406–4411.
Sun, B. B.; Chang, R.; Cao, S. P.; Yuan, C. Q.; Zhao, L. Y.; Yang, H. W.; Li, J. B.; Yan, X. H.; van Hest, J. C. M. Acid-activatable transmorphic peptide-based nanomaterials for photodynamic therapy. Angew. Chem., Int. Ed. 2020, 59, 20582–20588.
Bhangu, S. K.; Baral, A.; Zhu, H. Y.; Ashokkumar, M.; Cavalieri, F. Sound methods for the synthesis of nanoparticles from biological molecules. Nanoscale Adv. 2021, 3, 4907–4917.
Santha Kumar, A. R. S.; Padmakumar, A.; Kalita, U.; Samanta, S.; Baral, A.; Singha, N. K.; Ashokkumar, M.; Qiao, G. G. Ultrasonics in polymer science: Applications and challenges. Prog. Mater. Sci. 2023, 136, 101113.
De Castro, M. D. L.; Priego-Capote, F. Ultrasound-assisted crystallization (sonocrystallization). Ultrason. Sonochem. 2007, 14, 717–724.
D'Addio, S. M.; Prud'homme, R. K. Controlling drug nanoparticle formation by rapid precipitation. Adv. Drug Delivery Rev. 2011, 63, 417–426.
Joye, I. J.; McClements, D. J. Production of nanoparticles by anti-solvent precipitation for use in food systems. Trends Food Sci. Technol. 2013, 34, 109–123.
Ghosh, G.; Barman, R.; Mukherjee, A.; Ghosh, U.; Ghosh, S.; Fernández, G. Control over multiple nano- and secondary structures in peptide self-assembly. Angew. Chem., Int. Ed. 2022, 61, e202113403.
Zhang, L.; Jing, D.; Jiang, N.; Rojalin, T.; Baehr, C. M.; Zhang, D. L.; Xiao, W. W.; Wu, Y.; Cong, Z. Q.; Li, J. J. et al. Transformable peptide nanoparticles arrest HER2 signalling and cause cancer cell death in vivo. Nat. Nanotechnol. 2020, 15, 145–153.
Sis, M. J.; Ye, Z.; La Costa, K.; Webber, M. J. Energy landscapes of supramolecular peptide-drug conjugates directed by linker selection and drug topology. ACS Nano 2022, 16, 9546–9558.
Shai, Y. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by α-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochim. Biophys. Acta (BBA) - Biomembr. 1999, 1462, 55–70.
Kuang, Y.; Shi, J. F.; Li, J.; Yuan, D.; Alberti, K. A.; Xu, Q. B.; Xu, B. Pericellular hydrogel/nanonets inhibit cancer cells. Angew. Chem., Int. Ed. 2014, 53, 8104–8107.
Ding, Y. H.; Zheng, D. B.; Xie, L. M.; Zhang, X. Y.; Zhang, Z. H.; Wang, L.; Hu, Z. W.; Yang, Z. M. Enzyme-instructed peptide assembly favored by preorganization for cancer cell membrane engineering. J. Am. Chem. Soc. 2023, 145, 4366–4371.
Newcomb, C. J.; Sur, S.; Ortony, J. H.; Lee, O. S.; Matson, J. B.; Boekhoven, J.; Yu, J. M.; Schatz, G. C.; Stupp, S. I. Cell death versus cell survival instructed by supramolecular cohesion of nanostructures. Nat. Commun. 2014, 5, 3321.
Jeena, M. T.; Palanikumar, L.; Go, E. M.; Kim, I.; Kang, M. G.; Lee, S.; Park, S.; Choi, H.; Kim, C.; Jin, S. M. et al. Mitochondria localization induced self-assembly of peptide amphiphiles for cellular dysfunction. Nat. Commun. 2017, 8, 26.
Bhattacharjee, S.; Ershov, D.; Islam, M. A.; Kämpfer, A. M.; Maslowska, K. A.; van der Gucht, J.; Alink, G. M.; Marcelis, A. T. M.; Zuilhof, H.; Rietjens, I. M. C. M. Role of membrane disturbance and oxidative stress in the mode of action underlying the toxicity of differently charged polystyrene nanoparticles. RSC Adv. 2014, 4, 19321–19330.
Schnaider, L.; Brahmachari, S.; Schmidt, N. W.; Mensa, B.; Shaham-Niv, S.; Bychenko, D.; Adler-Abramovich, L.; Shimon, L. J. W.; Kolusheva, S.; DeGrado, W. F. et al. Self-assembling dipeptide antibacterial nanostructures with membrane disrupting activity. Nat. Commun. 2017, 8, 1365.
京公网安备11010802044758号