Abstract
This study provided visual evidence of a nitrogen effect on starch granules (SGs) in wheat endosperm. Winter wheat (Titicum aestivum L.) cultivar Xumai 30 was cultured under no nitrogen (control) and 240 kg ha-1 of nitrogen applied at the booting stage. The number, morphology, and size of A- and B-type SGs in subaleurone of dorsal endosperm (SDE), center of dorsal endosperm (CDE), modified aleurone (MA), subaleurone of ventral endosperm (SVE), and center of ventral endosperm (CVE) were observed under light and electron microscopes. (1) The distribution of SGs in SDE was similar to that in SVE, the distributions of SGs in CDE and CVE were similar, but the distribution of SGs in MA was different from those in the other four endosperm regions. The number of SGs in the five endosperm regions was in the order SDE > CDE > SVE > CVE > MA. (2) Nitrogen increased the number of A- and B-type SGs in SDE and SVE. Nitrogen also increased the number of B-type SGs but decreased the number of A-type SGs in CDE and CVE. Nitrogen decreased the numbers of A-type and B-type SGs in MA. The results suggest that increased N fertilizer application mainly increased the numbers of small SGs and decreased the numbers of large SGs, but that the results varied in different regions of the wheat endosperm.
