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Research paper | Open Access

Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity

Qiyan Jiang, Zheng Hu, Hui Zhang( ), Youzhi Ma( )
Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

The transcription factor dehydration-responsive element binding protein (DREB) is able to improve tolerance to abiotic stress in plants by regulating the expression of downstream genes involved in environmental stress resistance. The objectives of this study were to evaluate the salt tolerance of GmDREB1 transgenic wheat (Triticum aestivum L.) and to evaluate its physiological and protein responses to salt stress. Compared with the wild type, the transgenic lines overexpressing GmDREB1 showed longer coleoptiles and radicles and a greater radicle number at the germination stage, as well as greater root length, fresh weight, and tiller number per plant at the seedling stage. The yield-related traits of transgenic lines were also improved compared with the wild type, indicating enhanced salt tolerance in transgenic lines overexpressing GmDREB1. Proteomics analysis revealed that osmotic- and oxidative-stress-related proteins were up-regulated in transgenic wheat leaves under salt stress conditions. Transgenic wheat had higher levels of proline and betaine and lower levels of malondialdehyde and relative electrolyte leakage than the wild type. These results suggest that GmDREB1 regulates the expression of osmotic- and oxidative-stress-related proteins that reduce the occurrence of cell injury caused by high salinity, thus improving the salt tolerance of transgenic wheat.

Keywords

ProteomicsSalt toleranceTransgenic wheatGmDREB1

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The Crop Journal
Volume 2 Issue 2-3,
June 2014
Pages 120-131
DOI: 10.1016/j.cj.2014.02.003
Cite this article:
Jiang Q, Hu Z, Zhang H, et al. Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity. The Crop Journal, 2014, 2(2-3): 120-131. https://doi.org/10.1016/j.cj.2014.02.003

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Received: 16 October 2013
Revised: 06 January 2014
Accepted: 15 February 2014
Published: 28 February 2014
© 2014 Crop Science Society of China and Institute of Crop Science, CAAS. All rights reserved.
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