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Research paper | Open Access

Herbivore defense responses and associated herbivore defense mechanism as revealed by comparing a resistant wild soybean with a susceptible cultivar

Xiaoyi Wanga,b, Haifeng Chena, Zhihui Shana, Qingnan Haoa, Chanjuan Zhanga, Zhonglu Yanga, Xiaojuan Zhanga, Songli Yuana, Dezhen Qiua, Shuilian Chena, Yongqin Jiaoa, Xin'an Zhoua( )
Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Graduate School of the Chinese Academy of Agriculture Sciences, Beijing 100081, China

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS

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Abstract

Plants have evolved sophisticated defense mechanisms against herbivores to help them adapt to the environment. Understanding the defense mechanisms in plants can help us control insects in a more effective manner. In this study, we found that compared with Tianlong 2 (a cultivated soybean with insect susceptibility), ED059 (a wild soybean line with insect resistance) contains sharper pubescence tips, as well as lower transcript levels of wound-induced protein kinase (WIPK) and salicylic acid-induced protein kinase (SIPK), which are important mitogen-activated protein kinases involved in early defense response to herbivores. The observed lower transcript levels of WIPK and SIPK induced higher levels of jasmonic acid (JA), JA biosynthesis enzymes (AOC3) and some secondary metabolites in ED059. Functional analysis of the KTI1 gene via Agrobacterium-mediated transformation in Arabidopsis thaliana indicated that it plays an important role in herbivore defense in ED059. We further investigated the molecular response of third-instar Helicoverpa armigera (Hübner) larvae to Tianlong 2 and ED059. We found apoptotic cells only in the midguts of larvae that fed on ED059. Compared with larvae reared on the susceptible cultivar Tianlong 2, transcript levels of catalase (CAT) and glutathione S-transferase (GST) were up-regulated, whereas those of CAR, CHSB, and TRY were down-regulated in larvae that fed on the highly resistant variety ED059. We propose that these differences underlie the different herbivore defense responses of ED059 and Tianlong 2.

Keywords

MorphologySoybeanH. armigeraGene expression level polymorphism (ELP)Detoxification and toxin-tolerance gene

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The Crop Journal
Volume 3 Issue 6,
December 2015
Pages 451-467
DOI: 10.1016/j.cj.2015.07.001
Cite this article:
Wang X, Chen H, Shan Z, et al. Herbivore defense responses and associated herbivore defense mechanism as revealed by comparing a resistant wild soybean with a susceptible cultivar. The Crop Journal, 2015, 3(6): 451-467. https://doi.org/10.1016/j.cj.2015.07.001

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Received: 08 March 2015
Revised: 03 July 2015
Accepted: 08 July 2015
Published: 16 August 2015
© 2015 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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