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Research paper | Open Access

Physiological and molecular studies of staygreen caused by pod removal and seed injury in soybean

Xinxin Zhanga,b, Min Wangb, Tingting Wub, Cunxiang Wub, Bingjun Jiangb, Changhong Guoa(), Tianfu Hanb()
Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang, College of Life Science and Technology, Harbin Normal University, Harbin 150025, China
MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing 100081, China

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

Leaves provide substances and signals for pod and seed development in soybean. However, the regulatory feedbacks of pod and seed to leaf development remain unclear. We investigated the effects of pod and seed on leaf senescence by conducting pod removal and seed injury experiments. Pod removal and seed injury delayed leaf senescence and caused the staygreen phenotype of leaves. There were dosage effects of pod number on the extent of staygreen in depodded plants. The concentrations of chlorophyll (SPAD value, an index of relative chlorophyll content), soluble protein, and soluble sugar in the leaves of depodded plants were higher than those of intact plants. During seed development, the content of IAA decreased, while that of ABA increased. This trend was more pronounced in intact than in depodded and seed-injured plants. The GA3/ABA ratio decreased gradually in all treatments. The content of GA3 was relatively stable and was higher in intact than in depodded plants. The expression levels of four senescence-related genes, GmSARK, GmSGR1, GmCYN1, and GmNAC, declined in depodded or seed-injured treatments and were positively correlated with the number of leaves retained on plants. GmFT2a, the major flowering-promoting gene, was expressed at a higher level while E1, a key flowering inhibitory gene, was expressed at a lower level in depodded than in intact plants. We propose that the pod or seed can regulate leaf development. When the seed is aborted owing to disease infection or pest attack, the leaves stay green because of the absence of the seed signals for senescence.

Keywords

SoybeanSeed injuryStaygreenSource–sink relationship

References

[1]

Z. Dong, Soybean Yield Physiology, China Agricultural Press, Beijing, 2012 224 (in Chinese).

[2]

C. Turnbull, Long-distance regulation of flowering time, J. Exp. Bot. 62 (2011) 4399-4413.

Crossref Google Scholar
[3]

W.W. Garner, H.A. Allard, Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants, J. Agric. Res. 18 (1920) 553-606.

Google Scholar
[4]

T.F. Han, J.L. Wang, Studies on the post-flowering photoperiodic responses in soybean, Acta Bot. Sin. 37 (1995) 863-869 (in Chinese with English abstract).

Google Scholar
[5]

T.F. Han, J.Y. Gai, J.L. Wang, D.X. Zhou, Discovery of flowering reversion in soybean plants, Acta Agron. Sin. 24 (1998) 168-171 (in Chinese with English abstract).

Google Scholar
[6]

Z. Jia, C.X. Wu, M. Wang, H.B. Sun, W.S. Hou, B.J. Jiang, T.F. Han, Effects of leaf number of stock or scion in graft union on scion growth and development of soybean, Acta Agron. Sin. 37 (2011) 650-660 (in Chinese with English abstract).

Crossref Google Scholar
[7]

T. Koyama, The roles of ethylene and transcription factors in the regulation of onset of leaf senescence, Front. Plant Sci. 5 (2014) 650.

Crossref Google Scholar
[8]

X.P. Li, R. Gan, P.L. Li, Y.Y. Ma, L.W. Zhang, R. Zhang, Y. Wang, N.N. Wang, Identification and functional characterization of a leucine-rich repeat receptor-like kinase gene that is involved in regulation of soybean leaf senescence, Plant Mol. Biol. 61 (2006) 829-844.

Crossref Google Scholar
[9]

F. Xu, T. Meng, P.L. Li, Y.Q. Yu, Y.J. Cui, Y.X. Wang, Q.Q. Gong, N.N. Wang, A soybean dual-specificity kinase, GmSARK, and its Arabidopsis homolog, AtSARK, regulate leaf senescence through synergistic actions of auxin and ethylene, Plant Physiol. 157 (2011) 2131-2153.

Crossref Google Scholar
[10]

E. Breeze, E. Harrison, S. McHattie, L. Hughes, R. Hickman, C. Hill, S. Kiddle, Y.S. Kim, C.A. Penfold, D. Jenkins, C.J. Zhang, K. Morris, C. Jenner, S. Jackson, B. Thomas, A. Tabrett, R. Legaie, J.D. Moore, D.L. Wild, S. Ott, D. Rand, J. Beynon, K. Denby, A. Mead, V. Buchanan-Wollaston, High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation, Plant Cell 23 (2011) 873-894.

Crossref Google Scholar
[11]

J.D. Yang, E. Worley, M. Udvardi, A NAP-AAO3 regulatory module promotes chlorophyll degradation via ABA biosynthesis in Arabidopsis leaves, Plant Cell 26 (2014) 4862-4874.

Crossref Google Scholar
[12]

H. Takasaki, K. Maruyama, F. Takahashi, M. Fujita, T. Yoshida, K. Nakashima, F. Myouga, K. Toyooka, K. Yamaguchi-Shinozaki, K. Shinozaki, SNAC-As, stress-responsive NAC transcription factors, mediate ABA-inducible leaf senescence, Plant J. 84 (2015) 1114-1123.

Crossref Google Scholar
[13]

H. Thomas, C.J. Howarth, Five ways to stay green, J. Exp. Bot. 51 (2000) 329-337.

Crossref Google Scholar
[14]

Q. Du, L.K. Fang, X.C. Sang, Y.H. Ling, Y.F. Li, Z.L. Yang, G.H. He, F.M. Zhao, Analysis of phenotype and physiology of leaf apex dead mutant (lad) in rice and mapping of mutant gene, Acta Agron. Sin. 38 (2012) 168-173 (in Chinese with English abstract).

Crossref Google Scholar
[15]

G. Spano, N.D. Fonzo, C. Perrotta, C. Platani, G. Ronga, D.W. Lawlor, J.A. Napier, P.R. Shewry, Physiological characterization of ‘stay green’ mutants in durum wheat, J. Exp. Bot. 54 (2003) 1415-1420.

Crossref Google Scholar
[16]

M. Kusaba, A. Tanaka, R. Tanaka, Stay-green plants: what do they tell us about the molecular mechanism of leaf senescence, Photosynth. Res. 117 (2013) 221-234.

Crossref Google Scholar
[17]

C. Fang, C.C. Li, W.Y. Li, Z. Wang, Z.K. Zhou, Y.T. Shen, M. Wu, Y.S. Wu, G.Q. Li, L.A. Kong, C.M. Liu, S.A. Jackon, Z.X. Tian, Concerted evolution of D1 and D2 to regulate chlorophyll degradation in soybean, Plant J. 77 (2014) 700-712.

Crossref Google Scholar
[18]

X.M. Zhang, X.R. Ma, The treating measurements of Zhengqing disease of soybean, J. Henan Agric. Sci. 8 (2002) 21 (in Chinese).

Google Scholar
[19]

V.A. Wittenbach, Effect of pod removal on leaf senescence in soybeans, Plant Physiol. 70 (1982) 1544-1548.

Crossref Google Scholar
[20]

V.A. Wittenbach, Purification and characterization of a soybean leaf storage glycoprotein, Plant Physiol. 73 (1983) 125-129.

Crossref Google Scholar
[21]

P.E. Staswick, Developmental regulation and the influence of plant sinks on vegetative storage protein gene expression in soybean leaves, Plant Physiol. 89 (1989) 309-315.

Crossref Google Scholar
[22]
W.R. Fehr, C.E. Caviness, Stages of Soybean Development, Special Report/Agriculture and Home Economics ExperimentStation (No 80), Iowa State University, Ames, 1977 1–11.
[23]

F.J. Kong, B.H. Liu, Z.J. Xia, S. Sato, B.M. Kim, S. Watanabe, T. Yamada, S. Tabata, A. Kanazawa, K. Harada, J. Abe, Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in soybean, Plant Physiol. 154 (2010) 1220-1231.

Crossref Google Scholar
[24]

H.B. Sun, Z. Jia, D. Cao, B.J. Jiang, C.X. Wu, W.S. Hou, Y.K. Liu, Z.H. Fei, D.Z. Zhao, T.F. Han, GmFT2a, a soybean homolog of FLOWERING LOCUS T, is involved in flowering transition and maintenance, PLoS One 6 (2011) e29238.

Crossref Google Scholar
[25]

Z.J. Xia, S. Watanabe, T. Yamada, Y. Tsubokura, H. Nakashima, H. Zhai, T. Anai, S. Sato, T. Yamazaki, S.X. Lü, H.Y. Wu, S. Tabata, K. Harada, Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering, Proc. Natl. Acad. Sci. U. S. A. 109 (2012) E2155-E2164.

Crossref Google Scholar
[26]

X.H. Li, Y.H. Xie, X.F. Yang, Y.J. Wang, S.M. Ma, Dynamic changes of SPAD value of soybean leaves under different nitrogen concentrations, Hunan Agric. Sci. 10 (2013) 39-42 (in Chinese with English abstract).

Google Scholar
[27]

X.K. Wang, Principle and Technology of Plant Physiologicaland Biochemical Experiments, Higher Education Press,Beijing, 2006 190–191 (in Chinese).

Crossref
[28]

J. Zhao, G. Li, G.X. Yi, B.M. Wang, A.X. Deng, T.G. Nan, Z.H. Li, Q.X. Li, Comparison between conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA for small molecules, Anal. Chim. Acta 571 (2006) 79-85.

Crossref Google Scholar
[29]

B. Jian, B. Liu, Y.R. Bi, W.S. Hou, C.X. Wu, T.F. Han, Validation of internal control for gene expression study in soybean by quantitative real-time PCR, BMC Mol. Biol. 9 (2008) 59.

Crossref Google Scholar
[30]

Y.Y. Meng, H.Y. Li, Q. Wang, B. Liu, C.T. Lin, Blue light–dependent interaction between cryptochrome2 and CIB1 regulates transcription and leaf senescence in soybean, Plant Cell 25 (2013) 4405-4420.

Crossref Google Scholar
[31]

D.Q. Yang, Z.L. Wang, Y.P. Yin, Y.L. Ni, W.B. Yang, T. Cai, D.L. Peng, C.L. Xu, Z.Y. Cui, T.N. Liu, H.C. Xu, Effects of exogenous ABA and 6-BA on flag leaf senescence in different types of stay-green wheat and relevant physiological mechanisms, Acta Agron. Sin. 39 (2013) 1096-1104 (in Chinese with English abstract).

Crossref Google Scholar
[32]

L.D. Chang, W. Ma, J.Q. Guo, S.F. Fang, Control efficiency of spraying frequency on “Zhengqing” phenomenon of soybean, Heilongjiang Agric. Sci. 2 (2015) 53-55 (in Chinese with English abstract).

Google Scholar
[33]

X.Z. Li, Discussion on the causes and treating measurements of Zhengqing disease of soybean, J. Hebei Agric. Sci. 11 (2007) 64-65 (in Chinese).

Google Scholar
[34]

J.Q. Guo, W. Ma, Q.K. Lei, X.L. Yang, Y.X. Li, Tentative analysis of “Zhengqing” phenomena of soybean in the Huanghuai Valleys, J. Henan Agric. Sci. 41 (2012) 45-48 (in Chinese with English abstract).

Google Scholar
[35]

D.J. Boethel, J.S. Russin, A.T. Wier, M.B. Layton, J.S. Mink, M.L. Boyd, Delayed maturity associated with southern green stink bug (Heteroptera: Pentatomidae) injury at various soybean phenological stages, J. Econ. Entomol. 93 (2000) 707-712.

Crossref Google Scholar
[36]

L. Corbesier, C. Vincent, S. Jang, F. Fornara, Q. Fan, I. Searle, A. Giakountis, S. Farrona, L. Gissot, C. Turnbull, G. Coupland, FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis, Science 316 (2007) 1030-1033.

Crossref Google Scholar
[37]

S. Tamaki, S. Matsuo, H.L. Wong, S. Yokoi, K. Shimamoto, Hd3a protein is a mobile flowering signal in rice, Science 316 (2007) 1033-1036.

Crossref Google Scholar
[38]

T.L. Setter, W.A. Brun, Abscisic acid translocation and metabolism in soybeans following depodding and petiole girdling treatments, Plant Physiol. 67 (1981) 774-779.

Crossref Google Scholar
[39]

T.F. Han, J.Y. Gai, Phytohormonal analysis of some photoperiod effects in soybean, Acta Agron. Sin. 25 (1999) 349-355 (in Chinese with English abstract).

Google Scholar
The Crop Journal
Volume 4 Issue 6,
December 2016
Pages 435-443
DOI: 10.1016/j.cj.2016.04.002
Cite this article:
Zhang X, Wang M, Wu T, et al. Physiological and molecular studies of staygreen caused by pod removal and seed injury in soybean. The Crop Journal, 2016, 4(6): 435-443. https://doi.org/10.1016/j.cj.2016.04.002
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