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Research paper | Open Access

Drought-responsive genes expressed predominantly in root tissues are enriched with homotypic cis-regulatory clusters in promoters of major cereal crops

Muhammad Ramzan Khanb, Imran Khana, Zahra Ibrarc, Jens Léona, Ali Ahmed Naza( )
Institute of Crop Science and Resource Conservation (INRES), Department of Crop Genetics and Biotechnology, Rheinische Friedrich-Wilhelms University of Bonn, Germany
National Centre for Bioinformatics (NCB), Quaid-i-Azam University, Islamabad, Pakistan
National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Park Road, Islamabad, Pakistan

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

The root appears to be the most relevant organ for breeding drought stress tolerance. However, our knowledge about temporal and spatial regulation of drought-associated genes in the root remains fragmented, especially in crop plants. We performed a meta-analysis of expression divergence of essential drought-inducible genes and analyzed their association with cis-elements in model crops and major cereal crops. Our analysis of 42 selected drought-inducible genes revealed that these are expressed primarily in roots, followed by shoot, leaf, and inflorescence tissues, especially in wheat. Quantitative real-time RT-PCR analysis confirmed higher expression of TaDREB2 and TaAQP7 in roots, correlated with extensive rooting and drought-stress tolerance in wheat. A promoter scan up to 2kb upstream of the translation start site using phylogenetic footprinting revealed 708 transcription factor binding sites, including drought response elements (DREs), auxin response elements (AuxREs), MYCREs/MYBREs, ABAREs, and ERD1 in 19 selected genes. Interestingly, these elements were organized into clusters of overlapping transcription factor binding sites known as homotypic clusters (HCTs), which modulate drought physiology in plants. Taken together, these results revealed the expression preeminence of major drought-inducible genes in the root, suggesting its crucial role in drought adaptation. The occurrence of HCTs in drought-inducible genes highlights the putative evolutionary modifications of crop plants in developing drought adaptation. We propose that these DNA motifs can be used as molecular markers for breeding drought-resilient cultivars, particularly in the cereal crops.

Keywords

Gene expressionPhylogenyPromoterDREBcis-regulatory elementsDrought adaptation

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The Crop Journal
Volume 5 Issue 3,
June 2017
Pages 195-206
DOI: 10.1016/j.cj.2016.10.001
Cite this article:
Khan MR, Khan I, Ibrar Z, et al. Drought-responsive genes expressed predominantly in root tissues are enriched with homotypic cis-regulatory clusters in promoters of major cereal crops. The Crop Journal, 2017, 5(3): 195-206. https://doi.org/10.1016/j.cj.2016.10.001

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Received: 12 July 2016
Revised: 06 October 2016
Accepted: 21 October 2016
Published: 13 November 2016
© 2016 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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