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Research paper | Open Access

Transposon-based genetic diversity assessment in wild and cultivated barley

Surinder Singha,b,1,2, Prabhjot Singh Nandhaa,1, Jaswinder Singha( )
Department of Plant Science, McGill University, 21,111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada
Department of Plant Sciences, University of Saskatchewan, 51 campus drive, Saskatoon, SK S7N 5A8, Canada

1 Both these authors contributed equally as first authors.

2 Current address.

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

Transposable element-based molecular markers can be utilized to investigate genetic diversity and to create genetic linkage maps. In this study, Class I and class II transposons were employed to obtain a comparative account of genetic diversity between wild and cultivated barley genotypes. Three types of PCR-based techniques were used: IMP (Inter MITE Polymorphism), IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism). Specific primer pairs for IMP, IRAP, and REMAP detected a total of 200 bands with an average of 20 bands per marker. The mean polymorphic information content (PIC) and discrimination power (D) values in all 47 genotypes from these three types of transposon-based polymorphisms were 0.910 and 0.935, respectively. Unweighted Pair Group Method with Arithmetic mean (UPGMA)-based cluster analysis classified all 47 genotypes, both wild and cultivated, into separate groups consistent with their geographical origins. Sequencing followed by chromosome location of polymorphic bands enables precise gene introgression from wild gene pool to cultivated barley. The highly polymorphic nature of these marker systems makes them suitable for use in varietal identification and MAS-based breeding programs in barley and other cereals.

Keywords

IRAPIMPREMAPPolymorphic information content (PIC)Discrimination power (D)

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The Crop Journal
Volume 5 Issue 4,
August 2017
Pages 296-304
DOI: 10.1016/j.cj.2017.01.003
Cite this article:
Singh S, Nandha PS, Singh J. Transposon-based genetic diversity assessment in wild and cultivated barley. The Crop Journal, 2017, 5(4): 296-304. https://doi.org/10.1016/j.cj.2017.01.003

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Received: 26 October 2016
Revised: 12 January 2017
Accepted: 20 January 2017
Published: 08 March 2017
© 2017 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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