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Research paper | Open Access

Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation

Li Chena,b,1, Yupeng Caia,b,1, Xiujie Liua,b, Chen Guoa,b, Shi Sunb, Cunxiang Wub, Bingjun Jiangb, Tianfu Hanb, Wensheng Houa,b( )
National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

1 These authors contributed equally to this work.

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10–12days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.

Keywords

ProtocolTransformationSoybeanAgrobacterium rhizogenesHairy root

References

[1]
M.C. Christey, R.H. Braun, Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation, in: Leandro Peña (Ed.), Transgenic Plants: Methods and Protocols, Methods in Molecular Biology, Vol. 286, Humana Press, Totowa, New Jersey, USA 2005, pp. 47–60.
[2]

D. Cao, W.S. Hou, W. Liu, W.W. Yao, C.X. Wu, X.B. Liu, T.F. Han, Overexpression of TaNHX2 enhances salt tolerance of ‘composite’ and whole transgenic soybean plants, Plant Cell Tissue Organ Cult. 107 (2011) 541–552.
Crossref Google Scholar
[3]

J. Aarrouf, P. Castro-Quezada, S. Mallard, B. Caromel, Y. Lizzi, V. Lefebvre, Agrobacterium rhizogenes-dependent production of transformed roots from foliar explants of pepper (Capsicum annuum): a new and efficient tool for functional analysis of genes, Plant Cell Rep. 31 (2012) 391–401.
Crossref Google Scholar
[4]

B. Jian, W.S. Hou, C.X. Wu, B. Liu, W. Liu, S.K. Song, Y.R. Bi, T.F. Han, Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics, BMC Plant Biol. 9 (2009) 78.
Crossref Google Scholar
[5]

N. Bosselut, C.V. Ghelder, M. Claverie, R. Voisin, J.P. Onesto, M.N. Rosso, D. Esmenjaud, Agrobacterium rhizogenes-mediated transformation of Prunus as an alternative for gene functional analysis in hairy-roots and composite plants, Plant Cell Rep. 30 (2011) 1313–1326.
Crossref Google Scholar
[6]

S.R. Clemow, L. Clairmont, L.H. Madsen, F. Guinel, Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea, Plant Methods 7 (2011) 46.
Crossref Google Scholar
[7]

K.X. Tang, D.H. Liu, Y.L. Wang, L.J. Cui, W.W. Ren, X.F. Sun, Overexpression of transcriptional factor ORCA3 increases the accumulation of catharanthine and vindoline in Catharanthus roseus hairy roots, Russ. J. Plant Physiol. 58 (2011) 415–422.
Crossref Google Scholar
[8]

M.A.W. Hinchee, D.V. Connor-Ward, C.A. Newell, R.E. McDonnell, S.J. Sato, C.S. Gasser, D.A. Fischhoff, D.B. Re, R.T. Fraley, R.B. Horsch, Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer, Nat. Biotechnol. 6 (1988) 915–922.
Crossref Google Scholar
[9]

D.E. MaCabe, W.F. Swain, B.J. Martinell, P. Christou, Stable transformation of soybean (Glycine max) by particle acceleration, Nat. Biotechnol. 6 (1988) 923–926.
Crossref Google Scholar
[10]

L. Yu, X. Tan, B. Jiang, X. Sun, S. Gu, T. Han, W. Hou, A peroxisomal long-chain acyl-CoA synthetase from Glycine max involved in lipid degradation, PLoS One 9 (2014), e100144. .
Crossref Google Scholar
[11]

L. Chen, B.J. Jiang, C.X. Wu, S. Sun, W.S. Hou, T.F. Han, GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots, BMC Plant Biol. 14 (2014) 245.
Crossref Google Scholar
[12]

L. Chen, B.J. Jiang, C.X. Wu, S. Sun, W.S. Hou, T.F. Han, The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter, Plant Cell Tissue Organ Cult. 121 (2015) 259–274.
Crossref Google Scholar
[13]

Y.P. Cai, L. Chen, X.J. Liu, S. Sun, C.X. Wu, B.J. Jiang, T.F. Han, W.S. Hou, CRISPR/Cas9-mediated genome editing in soybean hairy roots, PLoS One 10 (2015), e0136064. .
Crossref Google Scholar
[14]

T.B. Jacobs, P.R. LaFayette, R.J. Schmitz, W. Parrott, Targeted genome modifications in soybean with CRISPR/Cas9, BMC Biotechnol. 15 (2015) 16.
Crossref Google Scholar
[15]

X.J. Sun, Z. Hu, R. Chen, Q.Y. Jiang, G.H. Song, H. Zhang, Y.J. Xi, Targeted mutagenesis in soybean using the CRISP-Cas9 system, Sci Rep 5 (2015) 10342.
Crossref Google Scholar
[16]

P. Costantino, L. Spano, M. Pomponi, E. Benvenuto, G. Ancora, The T-DNA of Agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants, J. Mol. Appl. Genet. 2 (1984) 465–470.
Google Scholar
[17]

R. Collier, B. Fuchs, N. Walter, W.K. Lutke, C.G. Taylor, Ex vitro composite plants: an inexpensive, rapid method for root biology, Plant J. 43 (2005) 449–457.
Crossref Google Scholar
[18]

R.A. Jefferson, Assaying chimeric genes in plants: the GUS gene fusion system, Plant Mol. Biol. Rep. 5 (1987) 387–405.
Crossref Google Scholar
[19]

V. Veena, C.G. Taylor, Agrobacterium rhizogenes: recent developments and promising applications, In Vitro Cell. Dev. Biol. Plant 43 (2007) 383–403.
Crossref Google Scholar
[20]

F. Bourgaud, A. Gravot, S. Milesi, E. Gontier, Production of plant secondary metabolites: a historical perspective, Plant Sci. 161 (2001) 835–891.
Crossref Google Scholar
[21]

M.I. Georgiev, A.I. Pavlov, T. Bley, Hairy root type plant in vitro systems as sources of bioactive substances, Appl. Microbiol. Biotechnol. 74 (2007) 1175–1185.
Crossref Google Scholar
[22]

S. Mehrotra, L.U. Rahman, A.K. Kukreja, An extensive case study of hairy-root cultures for enhanced secondary-metabolite production through metabolic-pathway engineering, Biotechnol. Appl. Biochem. 56 (2010) 161–172.
Crossref Google Scholar
[23]

Z.B. Hu, M. Du, Hairy root and its application in plant genetic engineering, J. Integr. Plant Biol. 48 (2006) 121–127.
Crossref Google Scholar
[24]

M. Ron, K. Kajala, G. Pauluzzi, D. Wang, M.A. Reynoso, K. Zumstein, J. Garcha, S. Winte, H. Masson, S. Inagaki, F. Federici, N. Sinha, R.B. Deal, J. Bailey-Serres, S.M. Brady, Hairy root transformation using Agrobacterium rhizogenes as a tool for exploring cell type-specific gene expression and function using tomato as a model, Plant Physiol. 166 (2014) 455–469.
Crossref Google Scholar
[25]

X.P. Qi, M.W. Li, M. Xie, X. Liu, M. Ni, G.H. Shao, C. Song, A.K.Y. Yim, Y. Tao, F.L. Wong, S. Isobe, C.F. Wong, K.S. Wong, C.Y. Xu, C.Q. Li, Y. Wang, R. Guan, F.M. Sun, G.Y. Fan, Z.X. Xiao, F. Zhou, T.H. Phang, X. Liu, S.W. Tong, T.F. Chan, S.M. Yiu, S. Tabata, J. Wang, X. Xu, H.M. Lam, Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing, Nat. Commun. 5 (2014) 4340.
Crossref Google Scholar
[26]

R. Rios-Estepa, B.M. Lange, Experimental and mathematical approaches to modeling plant metabolic networks, Phytochemistry 68 (2007) 2351–2374.
Crossref Google Scholar
[27]

N.N. Ono, L. Tian, The multiplicity of hairy root cultures: prolific possibilities, Plant Sci. 180 (2011) 439–446.
Crossref Google Scholar
[28]

S. Runo, S. Macharia, A. Alakonya, J. Machuka, N. Sinha, J. Scholes, Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions, Plant Methods 8 (2012) 20.
Crossref Google Scholar
[29]

A. Kereszt, D.X. Li, A. Indrasumunar, C.D.T. Nguyen, S. Nontachaiyapoom, M. Kinkema, P.M. Gresshoff, Agrobacterium rhizogenes-mediated transformation of soybean to study root biology, Nat. Protoc. 2 (2007) 948–952.
Crossref Google Scholar
[30]

M. Mohammadi-Dehcheshmeh, E. Ebrahimie, S.D. Tyerman, B.N. Kaiser, A novel method based on combination of semi-in vitro and in vivo conditions in Agrobacterium rhizogenes-mediated hairy root transformation of Glycine species, In Vitro Cell. Dev. Biol. Plant 50 (2014) 282–291.
Crossref Google Scholar
[31]

J.A. Kim, Y.S. Kim, Y.E. Choi, Triterpenoid production and phenotypic changes in hairy roots of Codonopsis lanceolata and the regenerated from them, Plant Biotechnol. Rep. 5 (2011) 255–263.
Crossref Google Scholar
[32]

Y.Q. Zhou, H.Y. Duan, C.E. Zhou, J.J. Li, F.P. Gu, F. Wang, Z.Y. Zhang, Z.M. Gao, Hairy root induction and plant regeneration of Rehmannia glutinosa Libosch.f.hueichingensis Hsiao via Agrobacterium rhizogenes-mediated transformation, Russ. J. Plant Physiol. 56 (2009) 224–231.
Crossref Google Scholar
[33]

C. Crane, E. Wright, R.A. Dixon, Z.Y. Wang, Transgenic Medicago truncatula plants obtained from Agrobacterium tumefaciens-transformed roots and Agrobacterium rhizogenes-transformed hairy roots, Planta 223 (2006) 1344–1354.
Crossref Google Scholar
[34]

P.M. Olhoft, L.M. Bernal, L.B. Grist, D.S. Hill, S.L. Mankin, Y. Shen, M. Kalogerakis, H. Wiley, E. Toren, H.S. Song, H. Hillebrand, T. Jones, A novel Agrabacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings, In Vitro Cell. Dev. Biol. Plant 43 (2007) 536–549.
Crossref Google Scholar
The Crop Journal
Volume 6 Issue 2,
April 2018
Pages 162-171
DOI: 10.1016/j.cj.2017.08.006
Cite this article:
Chen L, Cai Y, Liu X, et al. Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation. The Crop Journal, 2018, 6(2): 162-171. https://doi.org/10.1016/j.cj.2017.08.006

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Received: 31 May 2017
Revised: 24 August 2017
Accepted: 10 September 2017
Published: 05 October 2017
© 2017 “Crop Science Society of China and Institute of Crop Science, CAAS”.
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