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Research paper | Open Access

Characterization of a new hexaploid triticale 6D(6A) substitution line with increased grain weight and decreased spikelet number

Zhiyu Fenga, Zhongqi Qia, Dejie Dua, Mingyi Zhangb, Aiju Zhaoc, Zhaorong Hua, Mingming Xina, Yingyin Yaoa, Huiru Penga, Qixin Suna, Zhongfu Nia( )
Key Laboratory of Crop Heterosis and Utilization, State Key Laboratory for Agrobiotechnology, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen 041000, Shanxi, China
Hebei Crop Genetic Breeding Laboratory, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei, China

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

Hexaploid triticale (×Triticosecale, AABBRR) is an important forage crop and a promising energy plant. Transferring D-genome chromosomes or segments from common wheat (Triticum aestivum) into hexaploid triticale is attractive in improving its economically important traits. Here, a hexaploid triticale 6D(6A) substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and molecular markers. The GISH analysis showed that Lin 456 is a hexaploid triticale with 14 rye (Secale cereale) chromosomes and 28 wheat chromosomes, whereas non-denaturing fluorescence in situ hybridization (ND-FISH) and molecular marker analysis revealed that it is a 6D(6A) substitution line. In contrast to previous studies, the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456. The wheat chromosome 6D was associated with increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments. The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments. We propose that the hexaploid triticale 6D(6A) substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.

Keywords

Thousand-grain weightIn situ hybridizationSpikelet numberSubstitution lineTriticale

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The Crop Journal
Volume 7 Issue 5,
October 2019
Pages 598-607
DOI: 10.1016/j.cj.2019.03.007
Cite this article:
Feng Z, Qi Z, Du D, et al. Characterization of a new hexaploid triticale 6D(6A) substitution line with increased grain weight and decreased spikelet number. The Crop Journal, 2019, 7(5): 598-607. https://doi.org/10.1016/j.cj.2019.03.007

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Received: 02 December 2018
Revised: 16 March 2019
Accepted: 09 April 2019
Published: 06 June 2019
© 2019 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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