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Research paper | Open Access

Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system

Shiyu Wanga,b,1, Yihao Yanga,b,1, Min Guoa,c, Chongyuan Zhonga,b, Changjie Yanb,c( ), Shengyuan Suna,c( )
Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou 225009, Jiangsu, China
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou 225009, Jiangsu, China
Agricultural College of Yangzhou University, Yangzhou 225009, Jiangsu, China

1 Contributed equally to this work.

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

High grain protein content (GPC) reduces rice eating and cooking quality (ECQ). We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T0 generation, and GPC in the T1 generation decreased significantly, owing mainly to a reduction in glutelin content. Amylose content was down-regulated significantly in some Osaap6 and all Osaap10 mutants. The increased taste value of these mutants was supported by Rapid Visco Analysis (RVA) profiles, which showed higher peak viscosity and breakdown viscosity and lower setback viscosity than the wild type. There were no significant deficiencies in agronomic traits of the mutants. Targeted mutagenesis of OsAAP6 and OsAAP10, especially OsAAP10, using the CRISPR/Cas9 system can rapidly reduce GPC and improve ECQ of rice, providing a new strategy for the breeding cultivars with desired ECQ.

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The Crop Journal
Volume 8 Issue 3,
June 2020
Pages 457-464
DOI: 10.1016/j.cj.2020.02.005
Cite this article:
Wang S, Yang Y, Guo M, et al. Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system. The Crop Journal, 2020, 8(3): 457-464. https://doi.org/10.1016/j.cj.2020.02.005

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Received: 04 December 2019
Revised: 17 January 2020
Accepted: 19 February 2020
Published: 25 March 2020
© 2020 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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