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Short Communication | Open Access

Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat

Jia Leia,1, Jiawen Zhoua,1, Haojie Suna, Wentao Wana, Jin Xiaoa, Chunxia Yuana, Miroslava Karafiátováb, Jaroslav Doleželb, Haiyan Wanga( ), Xiue Wanga
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, Jiangsu, China
Institute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Hana for Biotechnological and Agricultural Research, Šlechtitelů 31, 77900 Olomouc, Czech Republic

1 These authors contributed equally to this work.

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement. Owing to the limited number of probes available for fluorescence in situ hybridization (FISH), the resolution at which the karyotype of H. villosa can be characterized is poor, hampering accurate characterization of small segmental alien introgressions. We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H. villosa chromosome 6V (6VS). FISH with seven of them resulted in clear signals on H. villosa chromosomes. Using these, we constructed FISH karyotypes for H. villosa using oligo-6VS-1 and oligo-6VS-35 oligonucleotides and characterized the distribution of the two probes in five different H. villosa accessions. The new FISH probes can efficiently characterize H. villosa introgressions into wheat.

Keywords

Chromosome identificationHaynaldia villosaOligonucleotide probesTandem DNA repeats

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The Crop Journal
Volume 8 Issue 4,
August 2020
Pages 676-681
DOI: 10.1016/j.cj.2020.02.008
Cite this article:
Lei J, Zhou J, Sun H, et al. Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat. The Crop Journal, 2020, 8(4): 676-681. https://doi.org/10.1016/j.cj.2020.02.008

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Received: 20 November 2019
Revised: 15 January 2020
Accepted: 04 March 2020
Published: 08 May 2020
© 2020 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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