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Research paper | Open Access

The heading-date gene Ghd7 inhibits seed germination by modulating the balance between abscisic acid and gibberellins

Yong Hua,b,1, Song Songb,1, Xiaoyu Wengb, Aiqing Youa( ), Yongzhong Xingb( )
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, Hubei, China

1 These authors contributed equally to this work.

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Abstract

Seed dormancy of cultivated rice was largely weakened during the progress of domestication. Correct timing and uniformity of seed germination are important for rapid seedling establishment and high-yield production. In the present study, we found that the heading-date gene Ghd7 acted as a negative regulator of germination. A mutant of ghd7 showed low sensitivity to exogenous ABA treatment during seed germination. Further investigation revealed reduced accumulation of ABA in mature ghd7 seeds as a consequence of dampened expression of OsNCED genes. Moreover, elevated GA3 level was detected in seeds of ghd7 mutant during imbibition course, which was attributed to the induction of genes responsible for the synthesis pathways of bioactive GAs. Thus, Ghd7 inhibits seed germination by increasing the ABA/GA3 ratio. Besides revealing pleiotropic effects of Ghd7, our results indicate its role in linking seed germination to growth-phase transition in rice, which would enrich the theoretical basis for future breeding practices.

Keywords

Abscisic acidSeed germinationGibberellinsGhd7ABA/GA3 ratio

References

[1]

W.E. Finch-Savage, G. Leubner-Metzger, Seed dormancy and the control of germination, New Phytol. 171 (2006) 501–523.
Crossref Google Scholar
[2]

R. Finkelstein, W. Reeves, T. Ariizumi, C. Steber, Molecular aspects of seed dormancy, Annu. Rev. Plant Biol. 59 (1) (2008) 387–415, https://doi.org/10.1146/annurev.arplant.59.032607.092740.
Crossref Google Scholar
[3]

Y. Liu, J. Fang, F. Xu, J. Chu, C. Yan, M.R. Schläppi, Y. Wang, C. Chu, Expression patterns of ABA and GA metabolism genes and hormone levels during rice seed development and imbibition: a comparison of dormant and non-dormant rice cultivars, J. Genet. Genomics 41 (2014) 327–338.
Crossref Google Scholar
[4]

J.D. Bewley, Seed germination and dormancy, Plant Cell 9 (1997) 1055–1066.
Crossref Google Scholar
[5]

A. Frey, D. Effroy, V. Lefebvre, M. Seo, F. Perreau, A. Berger, J. Sechet, A. To, H.M. North, A. Marion-Poll, Epoxycarotenoid cleavage by NCED5 fine-tunes ABA accumulation and affects seed dormancy and drought tolerance with other NCED family members, Plant J. 70 (2012) 501–512.
Crossref Google Scholar
[6]

G.K. Agrawal, M. Yamazaki, M. Kobayashi, R. Hirochika, A. Miyao, H. Hirochika, Screening of the rice viviparous mutants generated by endogenous retrotransposon Tos17 insertion. Tagging of a zeaxanthin epoxidase gene and a novel OsTATC gene, Plant Physiol. 125 (3) (2001) 1248–1257, https://doi.org/10.1104/pp.125.3.1248.
Crossref Google Scholar
[7]

C. Martinez-Andujar, M.I. Ordiz, Z. Huang, M. Nonogaki, R.N. Beachy, H. Nonogaki, Induction of 9-cis-epoxycarotenoid dioxygenase in Arabidopsis thaliana seeds enhances seed dormancy, Proc. Natl. Acad. Sci. 108 (41) (2011) 17225–17229, https://doi.org/10.1073/pnas.1112151108.
Crossref Google Scholar
[8]

M. Nonogaki, K. Sall, E. Nambara, H. Nonogaki, Amplification of ABA biosynthesis and signaling through a positive feedback mechanism in seeds, Plant J. 78 (3) (2014) 527–539, https://doi.org/10.1111/tpj.12472.
Crossref Google Scholar
[9]

B. Kucera, M.A. Cohn, G. Leubner-Metzger, Plant hormone interactions during seed dormancy release and germination, Seed Sci. Res. 15 (4) (2005) 281–307, https://doi.org/10.1079/SSR2005218.
Crossref Google Scholar
[10]

M. Okamoto, A. Kuwahara, M. Seo, T. Kushiro, T. Asami, N. Hirai, Y. Kamiya, T. Koshiba, E. Nambara, CYP707A1 and CYP707A2, which encode abscisic acid 8′-hydroxylases, are indispensable for proper control of seed dormancy and germination in arabidopsis, Plant Physiol. 141 (1) (2006) 97–107, https://doi.org/10.1104/pp.106.079475.
Crossref Google Scholar
[11]

F. Gubler, A.A. Millar, J.V. Jacobsen, Dormancy release, ABA and pre-harvest sprouting, Curr. Opin. Plant Biol. 8 (2) (2005) 183–187, https://doi.org/10.1016/j.pbi.2005.01.011.
Crossref Google Scholar
[12]

K. Graeber, A. Linkies, T. Steinbrecher, K. Mummenhoff, D. Tarkowská, V. Turečková, M. Ignatz, K. Sperber, A. Voegele, H. de Jong, T. Urbanová, M. Strnad, G. Leubner-Metzger, Delay of germination 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination, Proc. Natl. Acad. Sci. U. S. A. 111 (2014) E3571–E3580.
Crossref Google Scholar
[13]

M.J. Holdsworth, L. Bentsink, W.J.J. Soppe, Molecular networks regulating Arabidopsis seed maturation, after-ripening, dormancy and germination, New Phytol. 179 (2008) 33–54.
Crossref Google Scholar
[14]

S. Lee, H. Cheng, K.E. King, W. Wang, Y. He, A. Hussain, J. Lo, N.P. Harberd, J. Peng, Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI/RGA-like gene whose expression is up-regulated following imbibition, Genes Dev. 16 (2002) 646–658.
Crossref Google Scholar
[15]

K. Shu, H. Zhang, S. Wang, M. Chen, Y. Wu, S. Tang, C. Liu, Y. Feng, X. Cao, Q. Xie, ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in Arabidopsis, PLoS Genet. 9 (2013) e1003577.
Crossref Google Scholar
[16]

H. Ye, J. Feng, L. Zhang, J. Zhang, M.S. Mispan, Z. Cao, D.H. Beighley, J. Yang, X.Y. Gu, Map-based cloning of seed dormancy1-2 identified a gibberellin synthesis gene regulating the development of endosperm-imposed dormancy in rice, Plant Physiol. 169 (2015) 2152–2165.
Crossref Google Scholar
[17]

Y. Yamauchi, N. Takeda-Kamiya, A. Hanada, M. Ogawa, A. Kuwahara, M. Seo, Y. Kamiya, S. Yamaguchi, Contribution of gibberellin deactivation by AtGA2ox2 to the suppression of germination of dark-imbibed arabidopsis thaliana seeds, Plant Cell Physiol. 48 (2007) 555–561.
Crossref Google Scholar
[18]

C. Miao, Z. Wang, L. Zhang, J. Yao, K. Hua, X. Liu, H. Shi, J.K. Zhu, The grain yield modulator miR156 regulates seed dormancy through the gibberellin pathway in rice, Nat. Commun. 10 (2019) 3822.
Crossref Google Scholar
[19]

P.A. Tuan, R. Kumar, P.K. Rehal, P.K. Toora, B.T. Ayele, Molecular mechanisms underlying abscisic acid/gibberellin balance in the control of seed dormancy and germination in cereals, Front. Plant Sci. 9 (2018) 668.
Crossref Google Scholar
[20]

C.N. White, W.M. Proebsting, P. Hedden, C.J. Rivin, Gibberellins and seed development in maize. I. Evidence that gibberellin/abscisic acid balance governs germination versus maturation pathways, Plant Physiol. 122 (4) (2000) 1081–1088, https://doi.org/10.1104/pp.122.4.1081.
Crossref Google Scholar
[21]

M. Chen, D.R. MacGregor, A. Dave, H. Florance, K. Moore, K. Paszkiewicz, N. Smirnoff, I.A. Graham, S. Penfield, Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year, Proc. Natl. Acad. Sci. U. S. A. 111 (52) (2014) 18787–18792, https://doi.org/10.1073/pnas.1412274111.
Crossref Google Scholar
[22]

M. Chen, S. Penfield, Feedback regulation of COOLAIR expression controls seed dormancy and flowering time, Science 360 (6392) (2018) 1014–1017, https://doi.org/10.1126/science:aar7361.
Crossref Google Scholar
[23]

G.C.K. Chiang, D. Barua, E.M. Kramer, R.M. Amasino, K. Donohue, Major flowering time gene, FLOWERING LOCUS C, regulates seed germination in Arabidopsis thaliana, PNAS 106 (28) (2009) 11661–11666, https://doi.org/10.1073/pnas.0901367106.
Crossref Google Scholar
[24]

W. Xue, Y. Xing, X. Weng, Y. Zhao, W. Tang, L. Wang, H. Zhou, S. Yu, C. Xu, X. Li, Q. Zhang, Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice, Nat. Genet. 40 (6) (2008) 761–767, https://doi.org/10.1038/ng.143.
Crossref Google Scholar
[25]

X. Weng, L. Wang, J. Wang, Y. Hu, H. Du, C. Xu, Y. Xing, X. Li, J. Xiao, Q. Zhang, Grain number, plant height, and heading Date7 is a central regulator of growth, development, and stress response, Plant Physiol. 164 (2) (2014) 735–747, https://doi.org/10.1104/pp.113.231308.
Crossref Google Scholar
[26]

Y. Hu, S. Li, Y. Xing, Lessons from natural variations: artificially induced heading date variations for improvement of regional adaptation in rice, Theor. Appl. Genet. 132 (2019) 383–394.
Crossref Google Scholar
[27]

P.A. Counce, T.C. Keisling, A.J. Mitchell, A uniform, objective, and adaptive system for expressing rice development, Crop Sci. 40 (2) (2000) 436–443, https://doi.org/10.2135/cropsci2000.402436x.
Crossref Google Scholar
[28]

G. Wang, G. Wang, X. Zhang, F. Wang, R. Song, Isolation of high quality RNA from cereal seeds containing high levels of starch: RNA extraction from cereal seeds, Phytochem. Anal. 23 (2) (2012) 159–163, https://doi.org/10.1002/pca.1337.
Crossref Google Scholar
[29]

S. Song, G. Wang, H. Wu, X. Fan, L. Liang, H. Zhao, S. Li, Y. Hu, H. Liu, M. Ayaad, Y. Xing, OsMFT2 is involved in the regulation of ABA signaling‐mediated seed germination through interacting with OsbZIP23/66/72 in rice, Plant J. 103 (2) (2020) 532–546, https://doi.org/10.1111/tpj.14748.
Crossref Google Scholar
[30]

K. Shu, X.D. Liu, Q. Xie, Z.H. He, Two faces of one seed: hormonal regulation of dormancy and germination, Mol. Plant 9 (2016) 34–45.
Crossref Google Scholar
[31]

J. Fang, C. Chai, Q. Qian, C. Li, J. Tang, L. Sun, Z. Huang, X. Guo, C. Sun, M. Liu, Y. Zhang, Q. Lu, Y. Wang, C. Lu, B. Han, F. Chen, Z. Cheng, C. Chu, Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice, Plant J. 54 (2) (2008) 177–189, https://doi.org/10.1111/j.1365-313X.2008.03411.x.
Crossref Google Scholar
[32]

F. Liu, H. Zhang, G. Wu, J. Sun, L. Hao, X. Ge, J. Yu, W. Wang, Sequence variation and expression analysis of seed dormancy- and germination-associated ABA- and GA-related genes in rice cultivars, Front. Plant Sci. 2 (2011) 17.
Crossref Google Scholar
[33]

H. Saika, M. Okamoto, K. Miyoshi, T. Kushiro, S. Shinoda, Y. Jikumaru, M. Fujimoto, T. Arikawa, H. Takahashi, M. Ando, S.I. Arimura, A. Miyao, H. Hirochika, Y. Kamiya, N. Tsutsumi, E. Nambara, M. Nakazono, Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8′-hydroxylase in rice, Plant Cell Physiol. 48 (2007) 287–298.
Crossref Google Scholar
[34]

J. Yamaguchi, Analysis of embryo-specific α-amylase using isolated mature rice embryos, Jpn. J. Breed. 48 (1998) 365–370.
Crossref Google Scholar
[35]

H. Tsuji, K. Aya, M. Ueguchi-Tanaka, Y. Shimada, M. Nakazono, R. Watanabe, N.K. Nishizawa, K. Gomi, A. Shimada, H. Kitano, GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers, Plant J. 47 (2006) 427–444.
Crossref Google Scholar
[36]

S. Gazzarrini, A.Y.L. Tsai, Hormone cross-talk during seed germination, EssaysBiochem. 58 (2015) 151–164.
Crossref Google Scholar
[37]

H. Kim, H. Hwang, J.W. Hong, Y.N. Lee, I.P. Ahn, I.S. Yoon, S. Yoo, S. Lee, S.C. Lee, B.G. Kim, A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth, J. Exp. Bot. 63 (2011) 1013–1024.
Crossref Google Scholar
[38]

M.L. Fournier, A. Paulson, N. Pavelka, A.L. Mosley, K. Gaudenz, W.D. Bradford, E. Glynn, H. Li, M.E. Sardiu, B. Fleharty, C. Seidel, L. Florens, M.P. Washburn, Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin, Mol. Cell Proteomics 9 (2) (2010) 271–284, https://doi.org/10.1074/mcp.M900415-MCP200.
Crossref Google Scholar
[39]

L. Ponnala, Y. Wang, Q. Sun, K.J. van Wijk, Correlation of mRNA and protein abundance in the developing maize leaf, Plant J. 78 (3) (2014) 424–440, https://doi.org/10.1111/tpj.12482.
Crossref Google Scholar
[40]

X. He, J. Zhang, Toward a molecular understanding of pleiotropy, Genetics 173 (4) (2006) 1885–1891, https://doi.org/10.1534/genetics.106.060269.
Crossref Google Scholar
[41]

H. Itoh, Y. Nonoue, M. Yano, T. Izawa, A pair of floral regulators sets critical day length for Hd3a florigen expression in rice, Nat. Genet. 42 (2010) 635.
Crossref Google Scholar
The Crop Journal
Volume 9 Issue 2,
April 2021
Pages 297-304
DOI: 10.1016/j.cj.2020.09.004
Cite this article:
Hu Y, Song S, Weng X, et al. The heading-date gene Ghd7 inhibits seed germination by modulating the balance between abscisic acid and gibberellins. The Crop Journal, 2021, 9(2): 297-304. https://doi.org/10.1016/j.cj.2020.09.004

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Received: 22 April 2020
Revised: 29 July 2020
Accepted: 23 September 2020
Published: 17 November 2020
© 2020 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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