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Research paper | Open Access

Genetic mapping and identification of Lgf loci controlling green fuzz in Upland cotton (Gossypium hirsutum L.)

Dexin Liu, Xueying Liu, Yao Su, Xiao Zhang, Kai Guo, Zhonghua Teng, Jian Zhang, Dajun Liu, Zhengsheng Zhang( )
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
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Abstract

Naturally colored cotton fiber is environment-friendly but has monotonous color and poor fiber quality. Identification of green fiber or fuzz genes would aid in investigating the biosynthesis of green pigments in cotton fibers. In this study, we established a mapping population and found that the trait (white lint and green fuzz) from Gossypium hirsutum race latifolium is controlled by an incompletely dominant gene. The locus was mapped to a 71-kb interval on chromosome 21 containing seven genes, including a transcription factor with similarity to Arabidopsis MYB9. Harboring 13 SNPs and a 4-bp insertion/deletion in its promoter, GhMYB9 was highly up-regulated in the critical period for green pigment development in fuzz. Virus-induced gene silencing of GhMYB9 in a green-fuzz accession of G. hirsutum race latifolium TX-41 conferred white or light green fuzz. These results suggest that GhMYB9 is an important contributor to green pigments in cotton fiber and shed light on the regulatory mechanism controlling green pigmentation.

Keywords

Upland cottonGreen fuzzMYB9Green pigment

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The Crop Journal
Volume 9 Issue 4,
August 2021
Pages 777-784
DOI: 10.1016/j.cj.2020.09.009
Cite this article:
Liu D, Liu X, Su Y, et al. Genetic mapping and identification of Lgf loci controlling green fuzz in Upland cotton (Gossypium hirsutum L.). The Crop Journal, 2021, 9(4): 777-784. https://doi.org/10.1016/j.cj.2020.09.009

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Received: 27 February 2020
Revised: 09 July 2020
Accepted: 02 November 2020
Published: 25 November 2020
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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