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Research paper | Open Access

A natural allele of TAW1 contributes to high grain number and grain yield in rice

Hua Yuana,1, Zhengyan Xua,1, Xueqin Tana,1, Peng Gaoa, Mengya Jina, Wencheng Songa, Shiguang Wangb, Yunhai Kanga, Peixiong Liua, Bin Tua, Yuping Wanga, Peng Qina, Shigui Lia, Bingtian Maa( ), Weilan Chena( )
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China

1 These authors contributed equally to this work.

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Abstract

Grain number per panicle (GNP) is a complex trait controlled by quantitative trait loci (QTL), directly determining grain yield in rice. Identifying GNP-associated QTL is desirable for increasing rice yield. A rice chromosome segment substitution line (CSSL), F771, which showed increased panicle length and GNP, was identified in a set of CSSLs derived from a cross between two indica cultivars, R498 (recipient) and WY11327 (donor). Genetic analysis showed that the panicle traits in F771 were semidominant and controlled by multiple QTL. Six QTL were consistently identified by QTL-seq analysis. Among them, the major QTL qPLN10 for panicle length and GNP was localized to a 121-kb interval between markers N802 and N909 on chromosome 10. Based on quantitative real-time PCR and sequence analysis, TAWAWA1 (TAW1), a known regulator of rice inflorescence architecture, was identified as the candidate gene for qPLN10. A near-isogenic line, NIL-TAW1, was developed to evaluate its effects. In comparison with the recurrent parent R498, NIL-TAW1 showed increased panicle length (14.0%), number of secondary branches (20.9%) and GNP (22.0%), and the final grain yield per plant of NIL-TAW1 was increased by 18.6%. Transgenic experiments showed that an appropriate expression level of TAW1 was necessary for panicle development. Haplotype analysis suggested that the favorable F771-type (Hap 13) of TAW1 was introduced from aus accessions and had great potential value in high-yield breeding both in indica and japonica varieties. Our results provide a promising genetic resource for rice grain yield improvement.

Keywords

RiceQuantitative trait locusGrain number per paniclePanicle lengthTAW1

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The Crop Journal
Volume 9 Issue 5,
October 2021
Pages 1060-1069
DOI: 10.1016/j.cj.2020.11.004
Cite this article:
Yuan H, Xu Z, Tan X, et al. A natural allele of TAW1 contributes to high grain number and grain yield in rice. The Crop Journal, 2021, 9(5): 1060-1069. https://doi.org/10.1016/j.cj.2020.11.004

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Received: 07 September 2020
Revised: 16 October 2020
Accepted: 28 December 2020
Published: 09 January 2021
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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