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Research paper | Open Access

Heterogeneous expression of Osa-MIR156bc increases abiotic stress resistance and forage quality of alfalfa

Kexin Wanga, Yanrong Liua,c, Fengkui Tenga, Huifang Cena, Jianpin Yana, Shiwen Lina, Dayong Lid, Wanjun Zhanga,b( )
College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
Key Lab of Grassland Science in Beijing, China Agricultural University, Beijing 100193, China
College of Biological Science, China Agricultural University, Beijing 100193, China
College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong, China
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Abstract

Alfalfa (Medicago sativa L.) is the most widely cultivated perennial leguminous forage crop woldwide. MicroRNA156 (miR156) precursor genes from dicotyledonous species are reportedly useful for improving alfalfa plant architecture and abiotic stress resistance. However, there has been no report on whether a miR156 precursor gene from a monocotyledonous species functions in alfalfa. We introduced two tandem precursor genes of miR156, rice Osa-MIR156b and Osa-MIR156c (Osa-MIR156bc), into alfalfa. The expression of miR156 in the transgenic (TG) alfalfa was significantly elevated. Compared to wild-type plants, the TG plants overexpressing miR156 had more branches and leaves and showed improved salt and drought tolerance. Overexpression of miR156 slightly reduced plant height, but the biomass yield of TG plants grown in flowerpots was still increased. Forage quality of TG plants was markedly improved by reduction of acid detergent lignin (ADL) content and increase in crude protein content. The expression of the putative miR156 target genes MsSPL6, MsSPL12, and MsSPL13 in TG plants was repressed by miR156 overexpression, and that of all tested MsSPLs would be sharply increased under drought or salt stress. RNA sequencing revealed that overexpression of miR156 affected the expression of genes associated with abiotic stress resistance and plant development in multiple pathways. This first report of overexpression of monocot miR156 precursors in alfalfa sheds light on the function of miRNA156 precursors from the monocot species rice that could be used for genetic improvement of the dicot forage crop alfalfa.

Keywords

Abiotic stressAlfalfamiR156Biomass yieldForage quality

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The Crop Journal
Volume 9 Issue 5,
October 2021
Pages 1135-1144
DOI: 10.1016/j.cj.2020.11.009
Cite this article:
Wang K, Liu Y, Teng F, et al. Heterogeneous expression of Osa-MIR156bc increases abiotic stress resistance and forage quality of alfalfa. The Crop Journal, 2021, 9(5): 1135-1144. https://doi.org/10.1016/j.cj.2020.11.009

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Received: 13 August 2020
Revised: 12 October 2020
Accepted: 28 December 2020
Published: 12 January 2021
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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